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Application method of cyclodextrin-aldehyde cross-linking agent

A technology of aldehyde crosslinking agent and application method, which is applied in the field of cyclodextrin and collagen crosslinking agent, can solve the problems of not being able to obviously improve the shortcomings of collagen materials, the color of collagen materials, and the potential for calcification, etc., and achieve improved thermal stability , Enhanced degradation resistance, good cross-linking effect

Active Publication Date: 2013-10-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned chemical cross-linking agents can effectively improve the mechanical strength and stability of collagen, etc., but they also have some disadvantages through cross-linking, such as some will produce cytotoxicity, some will make the collagen material color, Some have calcification potential, some can not significantly improve some shortcomings of collagen materials, etc.

Method used

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  • Application method of cyclodextrin-aldehyde cross-linking agent
  • Application method of cyclodextrin-aldehyde cross-linking agent
  • Application method of cyclodextrin-aldehyde cross-linking agent

Examples

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preparation example Construction

[0032] The synthetic method of cyclodextrin polyaldehyde crosslinking agent of the present invention comprises the steps:

[0033] (1) Add 0.5 mmol of purified cyclodextrin into 5-8 ml of dimethyl sulfoxide, and completely dissolve the cyclodextrin to obtain a mixture;

[0034] (2) Add 2.2 to 2.8 equivalents of 2-iodylbenzoic acid to the mixture obtained in step (1), and react for 30 to 48 hours at 25 to 30°C while keeping stirring, then filter the reaction solution and keep filtrate;

[0035] (3) Drop the filtrate obtained in step (2) into acetone, and filter to retain the precipitate;

[0036] (4) Vacuum-dry the precipitate obtained in step (3) at 30-40°C for 24 hours, and then dissolve it with deionized water;

[0037] (5) Filter the solution obtained in step (4), retain the filtrate, and freeze-dry the filtrate for 48 hours to obtain the cyclodextrin polyaldehyde crosslinking agent.

Embodiment 1

[0039] (1) Preparation of cyclodextrin polyaldehyde crosslinking agent:

[0040] Weigh 0.5mmol of purified β-cyclodextrin in a 50ml flask, add 7ml of polar aprotic solvent dimethyl sulfoxide (DMSO) into the flask, magnetically stir the cyclodextrin until it is completely dissolved, then add 2.4 Equivalents of the mild oxidizing agent 2-iodobenzoic acid (IBX). Then react at 25°C for 48 hours under magnetic stirring, filter after the reaction, drop the filtrate into 150ml of acetone, filter the precipitated substance to obtain a precipitate, and dry the precipitate in a vacuum oven at 35°C for 24 hours , and finally the dried sample was dissolved with 50ml of deionized water, filtered to remove impurities, and the filtrate was freeze-dried to obtain a light yellow sample.

[0041] After testing, the yield of β-cyclodextrin polyaldehyde was 90%.

[0042] (2) Modified collagen:

[0043] The cyclodextrin polyaldehyde crosslinking agent prepared in step (1) was formulated into 5 ...

Embodiment 2

[0065] (1) Preparation of cyclodextrin polyaldehyde crosslinking agent:

[0066] Weigh 0.5mmol of purified γ-cyclodextrin in a 50ml flask, add 5ml of polar aprotic solvent dimethyl sulfoxide (DMSO) into the flask, magnetically stir the cyclodextrin until it is completely dissolved, then add 2.2 Equivalents of the mild oxidizing agent 2-iodobenzoic acid (IBX). Then react at 25°C for 30 hours under magnetic stirring, filter after the reaction, drop the filtrate into 150ml of acetone, filter the precipitated substance to obtain a precipitate, and dry the precipitate in a vacuum oven at 35°C for 24 hours , and finally the dried sample was dissolved with 50ml of deionized water, filtered to remove impurities, and the filtrate was freeze-dried to obtain the final sample.

[0067] After testing, the yield of cyclodextrin polyaldehyde was 87%.

[0068] (2) Modified collagen:

[0069] The cyclodextrin polyaldehyde crosslinking agent prepared in step (1) was formulated into 5 kinds o...

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Abstract

The invention discloses an application method of a cyclodextrin-aldehyde cross-linking agent. In the application method, the number of aldehyde groups in each mol of cyclodextrin-aldehyde cross-linking agent averagely is 2-3, so that the cyclodextrin-aldehyde cross-linking agent can be used as the cross-linking agent; and the cyclodextrin-aldehyde cross-linking agent has the advantages of nontoxicity and good cross-linking effect, and thereby is especially suitable for modification of collagen. According to the application method, the cyclodextrin-aldehyde cross-linking agent is applied to modification of the collagen material so that the thermal stability of the collagen material is greatly improved and the degradation resistance of the collagen material is obviously enhanced; some original characteristics of the collagen are maintained and some excellent properties of cyclodextrin are introduced; and therefore, the application range of the collagen is greatly expanded.

Description

technical field [0001] The invention relates to a cyclodextrin and collagen crosslinking agent, in particular to an application method of a cyclodextrin polyaldehyde crosslinking agent. Background technique [0002] Collagen is a biological natural polymer substance, the English scientific name collagen. Collagen exists in all cellular animals and is the most abundant type of protein in the body. It exists in almost all tissues and is a component of the extracellular matrix. Collagen has a special 3-strand helical peptide chain structure, which endows collagen with many good biological and chemical properties. Collagen has good biocompatibility, biodegradability, and low antigenicity; it has the functions of cell adaptability, cell proliferation and platelet aggregation; it has good solubility, non-toxicity, and unique fiber formation characteristics ; And the advantages of being easy to modify and having a wide range of sources. Therefore, collagen, as a very important b...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L89/00C08L5/16C08B37/16A61K6/898
CPCA61K8/65A61K8/738A61K38/39A61K2800/10A61L27/24A61L27/56A61Q19/00C08B37/0012C08H1/06C08K5/07C08L5/16
Inventor 刘卅蔡洁任力王迎军
Owner SOUTH CHINA UNIV OF TECH
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