Bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and application thereof

A technology of chlorsulfuron and carbendazim, applied in bacteria, microorganism-based methods, restoration of polluted soil, etc., can solve the problems of slow natural degradation process, carcinogenicity, mutagenicity, etc.

Active Publication Date: 2014-08-13
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carbendazim can cause human liver disease, lead to chromosomal aberration, has potential carcinogenic, teratogenic, and mutagenic effects, and is very toxic to mammals
[0011] Pesticide residues are mainly degraded by microorganisms in the soil, but the natural degradation process is very slow

Method used

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  • Bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and application thereof
  • Bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and application thereof
  • Bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, Isolation and Identification of Rhizobium sp. LD1616CGMCC No.7775

[0043] 1. Isolation of pesticide-degrading bacteria LD1616

[0044] Add 10 g of soil samples (collected from farmland polluted by the herbicide acetochlor in Beijing, China) to 100 mL of enrichment culture solution containing 20 mg / L of chlorimuron-methyl, and culture at 28°C and 180 r / min for 7 days to enrich chlorimuron Sulfur-methyl-degrading bacteria. After the first round of enrichment, 10 mL of the enrichment solution was transferred to 100 mL of the enrichment medium containing 40 mg / L of chlorimuron-methyl, and the culture was continued for 7 days for the second round of enrichment. Such continuous enrichment and culture for 5 times, the concentrations of chlorimuron-methyl were 20, 40, 60, 80 and 100 mg / L in turn.

[0045]The screening of degrading bacteria adopts the plate transparent circle method. Add chlorimuron-methyl to inorganic salt solid medium to make a plate, the final ...

Embodiment 2

[0067] Example 2, Quantitative determination of the ability of Rhizobium sp. LD1616CGMCC No.7775 to degrade pesticides chlorimuron-methyl and carbendazim

[0068] 1. Preparation of standard curve for the determination of chlorimuron-methyl

[0069] Use methanol to prepare chlorimuron-methyl standard substance (purchased from Fluka Company) into a series of standard solutions with concentrations of 10, 20, 40, 60, 80, and 100 mg / L, and use high performance liquid chromatography (HPLC) to determine different concentrations of chlorimuron-methyl The peak area of ​​the standard was repeated three times. With the concentration of chlorimuron-methyl as the abscissa and the peak area as the ordinate, draw the chlorimuron-methyl standard curve.

[0070] The detection conditions are as follows:

[0071] Detection system: Agilent1100Series. Chromatographic column: C18Diamosil TM Reversed-phase column, 250mm×4.6mm, particle size 5μm. Chromatographic conditions: mobile phase: acetoni...

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Abstract

The invention discloses a bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and an application of the bacterium. The bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim is rhizobium sp. LD1616 and the preservation number of the bacterium is CGMCC (China General Microbiological Culture Collection Center) No.7775. The rhizobium sp. LD1616 with the preservation of CGMCC NO.7775, disclosed by the invention, has the advantages that the degradation rate of the bacterium to chlorimuron-ethyl (45mg / L) is up to 71.56% in 7 days in an inorganic salt culture medium and the degradation rate of the bacterium to carbendazim (100mg / L) is up to 24.92% in 5 days, which indicates that the bacterium is capable of efficiently degrading the chlorimuron-ethyl and the carbendazim and has a broad application prospect in the remediation of soil polluted by residual pesticides chlorimuron-ethyl and carbendazim.

Description

technical field [0001] The invention relates to a bacterium for degrading pesticides chlorimuron-methyl and carbendazim and its application. Background technique [0002] Chlorimuron-methyl, English common name chlorimuron-ethyl, chemical name 2-(4-chloro-6-methoxypyrimidin-2-ylcarbamoylsulfamoyl) methyl benzoate, structural formula as shown in formula 1: [0003] [0004] Formula 1. [0005] Chlorimuron-methyl is an ultra-efficient sulfonylurea herbicide, and its target is ALS (acetolactate synthase) in plants, which inhibits the biosynthesis of branched-chain amino acids valine and isoleucine, resulting in the substrate α- The accumulation of butanone hinders the DNA synthesis in interphase of cell division, stops mitosis, and the cells cannot grow normally. Chlorimuron-methyl can be absorbed by the roots, stems, and leaves of plants, and conducts up and down in the plant body. It is a selective pre-emergence and post-emergence herbicide. It is mainly used in farmland...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/41A62D101/04A62D101/26
Inventor 孙建光解林奇高淼顾金刚
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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