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A kind of chemiluminescence substrate liquid of alkaline phosphatase

A luminescent substrate solution and chemiluminescent technology, which is applied in the field of immunoassay, can solve the problems of reducing the accuracy of temperature control and achieve low cost effects

Active Publication Date: 2016-08-17
JIANGSU ZECEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Analysis results are not sensitive to temperature in the range of 22°C--35°C, reducing the accuracy required for temperature control

Method used

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  • A kind of chemiluminescence substrate liquid of alkaline phosphatase
  • A kind of chemiluminescence substrate liquid of alkaline phosphatase
  • A kind of chemiluminescence substrate liquid of alkaline phosphatase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare 1000mL chemiluminescence substrate solution, the specific formula is as follows. Chemiluminescent substrate Luminescent substrate solution:

[0025]

[0026] Chemiluminescence substrate solution Luminescent substrate solution 1000mL preparation adopts the following method: take Tris31.3g, sodium sulfite 10mg, sodium lauryl sulfate 1g, N, N dimethylacridine nitrate 3.4mg, TWEEN-200.31g, Dissolve in purified water to 1000mL, pH9.35.

Embodiment 2

[0028] Compare the luminous intensity of the luminescent liquid described in the above-mentioned Example 1 with the commercially available imported APS-5 luminescent substrate liquid produced by Lumigen Company in the experiment of measuring carcinoembryonic antigen (CEA).

[0029] Experimental steps: Add 30ul of sample and calibrator to a flat-bottomed test tube, add 60ul of anti-reagent and mix well, incubate at 37°C for 30min, add 30ul of magnetic particle reagent and incubate at 37°C for 5min. Wash 3 times with washing solution, put into a semi-automatic luminometer, add 200ul luminescence substrate to each tube to test the luminescence value.

[0030] The APS-5 luminescent substrate solution produced by Lumigen Company and the luminescent substrate solution in Example 1 were used to test the calibrator respectively, and the results are as follows:

[0031] Table 1 Luminescence value table of different calibration substance concentrations of the imported control luminescen...

Embodiment 3

[0051] The luminescent substrate solution described in the above-mentioned Example 1 was compared with the commercially available imported APS-5 luminescent substrate solution produced by Lumigen Company to determine the luminescence plateau stage in the carcinoembryonic antigen (CEA) experiment.

[0052] Add 30ul each of the sample and the calibrator to a flat-bottomed test tube, add 60ul of anti-reagent and mix well, incubate at 37°C for 30min, add 30ul of magnetic particle reagent and incubate at 37°C for 5min. Wash 3 times with lotion, put it into a semi-automatic luminometer, add 200ul luminescent substrate to each tube, measure once every 30 seconds, test continuously for 10 minutes, and count the changes of the luminescence value of the same sample within 10 minutes.

[0053] The imported luminescent substrate and the luminescent substrate solution described in Example 1 were used for experiments respectively.

[0054] The result is as follows:

[0055] Table 3 The rel...

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PUM

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Abstract

The invention discloses a chemiluminiscence substrate liquid capable of being applied to the field of immune detection. The chemiluminiscence substrate liquid contains 9-(4-chlorphenyl sulfobenzoyloyl methylene)-10-methyl-9, 10-dihydroacridine-disodium salt, N, N-dimethyl acridine nitrate, trihydroxymethyl amino methane, sodium sulfite, lauryl sodium sulfate and TWEEN-20. The chemiluminiscence substrate liquid disclosed by the invention is high in sensitivity, short in platform time, long in stable period of a platform and high in stability.

Description

technical field [0001] The invention relates to the field of immune analysis, and can be applied to the chemiluminescence substrate liquid in the field of immune detection. Background technique [0002] Chemiluminescence is a kind of light radiation phenomenon accompanied by the chemical reaction of substances. When some substances undergo a chemical reaction, they absorb the chemical energy generated during the reaction, so that the reaction product molecules are excited to an electronically excited state. When electrons return from the lowest vibrational energy level of the excited state to the various vibrational energy levels of the ground state, radiation is produced, and the excess energy is released in the form of photons. This phenomenon is called chemiluminescence. Chemiluminescence immunoassay (CLIA), which combines high-sensitivity chemiluminescence assay technology with high-specificity immune response, is used for various antigens, haptens, antibodies, hormones...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/52
Inventor 夏振伟丁建华余卫国杨旻
Owner JIANGSU ZECEN BIOTECH CO LTD