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Control method for microbial fermentation production of gluconate

A microbial fermentation method and gluconate technology, applied in the field of bioengineering, can solve the problems of adjusting the air intake and stirring speed of the fermentation tank, lack of fermentation tanks, etc.

Inactive Publication Date: 2013-10-16
山东福洋生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the fermentation of gluconic acid and its salts (such as sodium gluconate, calcium gluconate, etc.) However, none of these methods can automatically adjust the air intake and stirring speed of the fermenter to ensure sufficient dissolved oxygen concentration (DO), so that the fermentation oxygen consumption rate (OUR) has always been at the best level
[0005] To sum up, there is currently a lack of methods in the art that can automatically adjust the air intake and stirring speed of the fermentation tank through online closed-loop control during the fermentation process of calcium gluconate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The second step, fermentation: Add glucose 4.0 kg, magnesium sulfate 2.8g, urea 1.4g, defoamer 2ml to a 50L fermenter, set the volume to 26L, sterilize at 115°C for 15 minutes, then lower it to 38°C, and use 30% The calcium oxide suspension was adjusted to control pH5.8, and fermentation began. During the fermentation process, the air volume and stirring speed of the fermenter are automatically adjusted according to the OUR detected online. When the OUR in the fermentation broth is less than or equal to 40±1, the air volume and speed are automatically increased every 5 minutes, and each increase is 50L / h and 20r / min, increase alternately; when OUR≥50±1, reduce the air intake or stirring speed, each reduction is 25L / h and 10r / min, alternately decrease.

[0012] Fermentation ended when DO rose to 100.

[0013] The third step, filtering: plate and frame filtering.

[0014] The fourth step, decolorization: activated carbon decolorization.

[0015] The fifth step, crystal...

Embodiment 2

[0020] The second step, fermentation: add 4.0kg of glucose, 2.8g of magnesium sulfate, 1.4g of urea, and 2ml of defoamer to a 50L fermenter, set the volume to 26L, sterilize at 115°C for 15 minutes, then lower it to 38°C, and use 32% The sodium hydroxide is adjusted to control the pH5.8, and the fermentation is started. During the fermentation process, the air volume and stirring speed of the fermenter are automatically adjusted according to the OUR detected online. When the OUR in the fermentation broth is less than or equal to 40±1, the air volume and speed are automatically increased every 5 minutes, and each increase is 50L / h and 20r / min, increase alternately; when OUR≥50±1, reduce the air intake or stirring speed, each reduction is 25L / h and 10r / min, alternately decrease.

[0021] Fermentation ended when DO rose to 100.

[0022] The third step, filtering: plate and frame filtering.

[0023] The fourth step, decolorization: activated carbon decolorization.

[0024] The...

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PUM

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Abstract

The invention discloses a control method for microbial fermentation production of gluconate. A glucose acid high-producing strain is adopted as a starting strain, after seed culture, the strain is transferred into a fermentation tank to undergo fermentation production of gluconate. During fermentation, the fermentation tank air inflow rate and stirring speed can be adjusted automatically according to an on-line measured oxygen uptake rate (OUR). Thus, an enough dissolved oxygen concentration is guaranteed, the acid production rate is enhanced, and direct, real-time, and accurate control of a gluconate fermentation process can be realized.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for controlling the production of gluconate by microbial fermentation. Background technique [0002] Gluconic acid is widely used in food, medicine, chemical industry, water treatment, construction and other industries as a bulking agent, coagulant, chelating agent, and sour agent; gluconate (such as with sodium, calcium, zinc, ferrous and other metal oxides) Synthetic gluconate) can be used as food additives and nutritional supplements. [0003] The production of gluconic acid mainly includes microbial fermentation, electrolysis and catalytic oxidation. Due to the mild conditions and obvious energy saving of the microbial method, it is widely used at present. Among them, microbial fermentation includes fungal fermentation and bacterial fermentation process. Since Boutroux first used the oxidation of microorganisms to oxidize glucose into gluconic acid, the fermentation p...

Claims

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Application Information

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IPC IPC(8): C12Q3/00
Inventor 赵伟张雷达王健曹大鹏宋金松杨健
Owner 山东福洋生物科技股份有限公司
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