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36results about How to "Increase the rate of acid production" patented technology

Method for producing table vinegar by adopting two-step acetic acid fermentation method

The invention discloses a method for producing table vinegar by adopting a two-step acetic acid fermentation method and belongs to the technical field of brewing of the table vinegar. According to the method, the problems of low alcohol intolerance, long fermentation period and low acetification rate for acetic acid fermentation in a traditional method are solved. The method comprises the following steps of: uniformly stirring sorghum flour, corn flour, rice meal and water; adding thermostable alpha amylase for liquefying; after liquefying, reducing the temperature, adding Daqu, saccharifyingkoji, red starter, saccharomyces cerevisiae and aroma-producing yeast and carrying out alcoholic fermentation to obtain liquor; adopting the two-step acetic acid fermentation method: firstly, mixing the liquor; adding acetic acid bacteria subjected to liquid amplification culture for carrying out primary acetic acid fermentation; when the alcoholic strength of brewing mass is reduced to 6-5DEG C,adding wheat bran and rice husk as accessories for mixing; inoculating fire fermented liquor for carrying out secondary acetic acid fermentation; sealing the liquor; smoking the liquor; spraying vinegar; filtering; and packaging to obtain a finished product. According to the method disclosed by the invention, the high-content alcohol can be effectively converted; the acid yield and the acetification rate in unit time are improved; and the flavor of the table vinegar produced by adopting a traditional solid acetic acid fermentation method is ensured.
Owner:山西金龙鱼梁汾醋业有限公司

Short-chain volatile fatty acid and preparation method thereof

The invention discloses a short-chain volatile fatty acid and a preparation method thereof. The preparation method for the short-chain volatile fatty acid comprises the following steps of employing sludge as a fermentation substrate; adding sodium nitrite into the fermentation substrate, performing pretreatment in an acid environment, and performing anaerobic fermentation under an alkaline condition to obtain the short-chain volatile fatty acid. According to the short-chain volatile fatty acid and the preparation method thereof, the sludge of a sewage treatment plant is taken as a raw material, and is jointly treated by virtue of free nitrite pretreatment and alkaline anaerobic fermentation, and the concentration of free nitrite in the system, the pretreatment time, the fermentation pH value and the action of microorganisms in the sludge are controlled to maximally convert undissolved sludge organic matters in the sludge into the short-chain volatile fatty acid, so that the yield of the volatile fatty acid produced from the sludge is further increased on the basis of resourcefully utilizing the sludge and reducing environmental pollution caused by the sludge, and the reaction time is shortened; the short-chain volatile fatty acid and the preparation method thereof have the advantages of high product yield, simple preparation method, energy conservation, consumption reduction, low running cost and the like.
Owner:HUNAN UNIV

Method of optimizing acid production structure through pretreatment of iron electrode activation of persulfate

The invention discloses a method of optimizing an acid production structure in an anaerobic fermentation acid production process of excess sludge and increasing a short chain fatty acid yield throughpretreatment of iron anode and electricity combined activation of persulfate. The method comprises the steps of: taking sludge as a fermentation substrate, a single-chamber electrolytic tank as a reaction device, an iron sheet as an anode and a graphite rod as a cathode, adding the sludge into an electrochemical reaction tank, adding persulfate into the fermentation substrate at the same time, electrifying for pretreatment, adjusting an initial pH (potential of hydrogen) to 10 for anaerobic fermentation, and obtaining structure optimized short chain volatile fatty acid. The method takes the sludge of a sewage treatment plant as a raw material; an acetic acid yield can be increased to the maximum extent by controlling an iron concentration, a current magnitude, electrifying time and a persulfate concentration in a pretreatment system; and the method contributes to achieving carbon source internal circulation of the sewage treatment plant and promoting sludge reclamation utilization. Atthe same time, the method also has the advantages of simplicity in operation, convenient management and high cost performance.
Owner:HUNAN UNIV

Method for producing table vinegar by adopting two-step acetic acid fermentation method

The invention discloses a method for producing table vinegar by adopting a two-step acetic acid fermentation method and belongs to the technical field of brewing of the table vinegar. According to the method, the problems of low alcohol intolerance, long fermentation period and low acetification rate for acetic acid fermentation in a traditional method are solved. The method comprises the following steps of: uniformly stirring sorghum flour, corn flour, rice meal and water; adding thermostable alpha amylase for liquefying; after liquefying, reducing the temperature, adding Daqu, saccharifying koji, red starter, saccharomyces cerevisiae and aroma-producing yeast and carrying out alcoholic fermentation to obtain liquor; adopting the two-step acetic acid fermentation method: firstly, mixing the liquor; adding acetic acid bacteria subjected to liquid amplification culture for carrying out primary acetic acid fermentation; when the alcoholic strength of brewing mass is reduced to 6-5DEG C, adding wheat bran and rice husk as accessories for mixing; inoculating fire fermented liquor for carrying out secondary acetic acid fermentation; sealing the liquor; smoking the liquor; spraying vinegar; filtering; and packaging to obtain a finished product. According to the method disclosed by the invention, the high-content alcohol can be effectively converted; the acid yield and the acetification rate in unit time are improved; and the flavor of the table vinegar produced by adopting a traditional solid acetic acid fermentation method is ensured.
Owner:山西金龙鱼梁汾醋业有限公司

A genetically engineered bacterium producing α-ketobutyric acid and its application

The invention relates to a genetically engineered bacterium for producing alpha-ketobutyric acid as well as a construction method and application of the genetically engineered bacterium, belonging to the technical field of biology. The strain is relatively high in production efficiency when used for producing alpha-ketobutyric acid through fermentation. The genetically engineered bacterium is obtained through carrying out genetic engineering modification on Escherichia coli MG1655; the genetic engineering modification is realized by knocking out a large-subunit encoding gene ilvB of acetohydroxyacid synthase I, a large-subunit encoding gene ilvI of acetohydroxyacid synthase III and a threonine operon leading peptide encoding gene thrL in an over-expressive threonine dehydratase encoding gene ilvA. When the bacterium is used for producing alpha-ketobutyric acid by using a fermentation method, defects such as complex reaction conditions, high energy consumption, serious pollution or high production cost, serious pollution and the like existing in a chemical synthesis method, an enzyme method or a microbial conversion method can be overcome; after the bacterium is fermented for 20-24h, the yield of alpha-ketobutyric acid is up to 8.5-15.7g / L; aerobic fermentation is adopted in a fermentation process; the bacterium is rapid in growth, short in fermentation period and high in acid production rate; any reports for producing alpha-ketobutyric acid by using a direct fermentation method are not found at present.
Owner:江苏澳创生物科技有限公司
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