A kind of Aspergillus niger seed continuous culture and method for producing citric acid thereof

A technology of Aspergillus niger spores and Aspergillus niger, which is applied in the field of continuous cultivation of Aspergillus niger seeds and the production of citric acid, which can solve the problems of slow growth of multicellular filamentous bacteria and easy loss of mycelium balls, and reduce the auxiliary time of starting and stopping , Auxiliary time saving, stable fermentation index effect

Active Publication Date: 2020-06-05
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of the present invention solves the problem of slow growth of multicellular filamentous bacteria and easy loss of mycelium balls in continuous culture, completely realizes the continuous cultivation of seeds, keeps the bacteria in the most suitable growth environment, and avoids the problem of bacterial growth. Seed degradation, so that the seed liquid is in a continuous and stable state of high vitality, and the corresponding fermentation index is significantly improved

Method used

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  • A kind of Aspergillus niger seed continuous culture and method for producing citric acid thereof
  • A kind of Aspergillus niger seed continuous culture and method for producing citric acid thereof
  • A kind of Aspergillus niger seed continuous culture and method for producing citric acid thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Corn flour and tap water are mixed evenly in a mass ratio of 1:3, and Ca(OH) 2 Adjust the pH of the slurry to 6.0, and add α-high-temperature amylase according to the amount of 20U / g corn flour; after spraying and liquefying, the iodine test turns light brown to obtain a qualified corn liquefaction liquid; pass 80% of the corn liquefaction liquid through the plate frame Filter and remove the filter residue to obtain corn syrup.

[0039] Start-up stage: the corn liquefaction solution and ammonium sulfate are prepared into a seed medium (total sugar 100g / L, C / N is 20), after sterilization and cooling, Aspergillus niger spores are inoculated into the seed medium, and the final concentration of spores is 9*10 5 cells / mL, cultured for 16 hours at a temperature of 35°C, an air volume of 0.2vvm, a tank pressure of 0.05Mpa, and a stirring speed of 100rpm to obtain a seed liquid.

[0040] Continuous stage: The corn liquefaction liquid and ammonium sulfate are prepared into a se...

Embodiment 2

[0044] Corn flour and tap water are mixed evenly in a mass ratio of 1:3, and Ca(OH) 2 Adjust the pH of the slurry to 6.0, and add α-high-temperature amylase according to the amount of 20U / g corn flour; after spraying and liquefying, the iodine test turns light brown to obtain a qualified corn liquefaction liquid; pass 80% of the corn liquefaction liquid through the plate frame Filter and remove the filter residue to obtain corn syrup. Cassava flour and tap water are mixed evenly in a mass ratio of 1:3, and Ca(OH) 2 Adjust the pH of the slurry to 6.0, and add α-high temperature amylase according to the amount of 20U / g cassava flour; after jet liquefaction, the iodine test turns light brown to obtain a qualified cassava syrup.

[0045] Start-up stage: The corn liquefaction solution and urea are prepared into the seed medium (total sugar 140g / L, C / N is 30), after sterilization and cooling, the Aspergillus niger spores are inoculated into the seed medium, and the final concentrat...

Embodiment 3

[0050] Corn flour and tap water are mixed evenly in a mass ratio of 1:3, and Ca(OH) 2 Adjust the pH of the slurry to 6.0, and add α-high-temperature amylase according to the amount of 20U / g corn flour; after spraying and liquefying, the iodine test turns light brown to obtain a qualified corn liquefaction liquid; pass 80% of the corn liquefaction liquid through the plate frame Filter and remove the filter residue to obtain corn syrup. Wheat starch and tap water are mixed evenly in a mass ratio of 1:3, and Ca(OH) 2 Adjust the pH of the slurry to 6.0, and add α-high temperature amylase according to the amount of 20U / g wheat starch; after jet liquefaction, the iodine test turns light brown to obtain qualified wheat sugar liquid.

[0051] Start-up stage: Prepare corn liquefaction liquid and soybean meal powder to make seed medium (total sugar 180g / L, C / N is 40), inoculate Aspergillus niger spores into the seed medium after sterilization and cooling, the final concentration of spo...

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Abstract

The invention discloses a method for continuous cultivation of Aspergillus niger seeds. The method comprises the following steps: (1) in the start-up stage, inoculating the Aspergillus niger spores into the seed medium to obtain a seed liquid; (2) in the continuous cultivation stage of the seeds, The seed solution obtained in step (1) is subjected to continuous dispersion treatment, and the seed solution obtained by dispersing is continuously cultivated, and fresh seed feed medium is supplemented at the same time; (3) stop stage, stop supplementing fresh seed feed medium and disperse treatment, Continue culturing to obtain seed liquid, which is transferred into the fermentation medium for fermentation and cultivation. The method of the present invention solves the problem of slow growth of multicellular filamentous bacteria and easy loss of mycelium balls in continuous culture, completely realizes the continuous cultivation of seeds, keeps the bacteria in the most suitable growth environment, and avoids the Seed degradation, so that the seed liquid is in a continuous and stable high-activity state, corresponding to the improvement of the performance of citric acid production by fermentation.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a continuous culture of Aspergillus niger seeds and a method for producing citric acid. Background technique [0002] Citric acid is the most produced and used organic acid in the world, and it is an important chemical product with a wide range of uses. Citric acid is mainly used in the food industry, such as sour agent, antioxidant, gelling agent, etc., and has a wide range of applications in pharmaceutical, feed, chemical, electronics, textile and other industrial fields, and the market demand is increasing year by year. my country is the largest producer and exporter of citric acid, with advanced production technology and strong market competitiveness, especially the submerged fermentation index created by corn, dried potato and other starchy raw materials ranks among the top in the world. [0003] Although bio-fermentation technology has been developing rapidly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12N1/14C12R1/685
CPCC12N1/14C12P7/48C12R2001/685C12N1/145C12N1/04
Inventor 石贵阳胡志杰李由然蒋小东金赛孙福新张成周东姣卢佳伟苗茂栋范子豪
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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