Biomembrane-electrodialysis coupling continuous production technology of L-lactic acid

A technology of biofilm and electrodialysis, which is applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low concentration and easy shedding of cells, achieve high sugar yield, and avoid acid and salt wastewater , the effect of reducing production costs

Active Publication Date: 2011-05-04
CHINA PETROLEUM & CHEM CORP +1
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  • Description
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  • Application Information

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Problems solved by technology

The adsorption method is relatively simple and can have various forms (rotary disk, rotating tube, granular, etc.), but the cells are easy to fall off and the concentration is low

Method used

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  • Biomembrane-electrodialysis coupling continuous production technology of L-lactic acid
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Examples

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preparation example Construction

[0026] (1) Preparation of saccharification solution: sugar-containing substances are saccharified by low-temperature double-enzyme method, filtered with a plate and frame filter to obtain saccharification solution, and the filter residue can be used as fibrin feed. The concentration of the obtained saccharified liquid is generally 130-200 g / L.

[0027] The sugar-containing substance refers to sugar-containing crops, preferably corn, wheat, sweet potato, potato or molasses; wherein corn, wheat, sweet potato and potato need to be pulverized first, and then amylase is added for hydrolysis.

[0028] (2) Preparation of nutrient substance: the nutrient substance is preferably soybean meal hydrolyzate, bran and corn steep liquor; wherein the preparation method of soybean meal hydrolyzate is: hydrolyze soybean meal with sulfuric acid to obtain soybean meal hydrolyzate as nitrogen source.

[0029] (3) Activate the lactic acid-producing bacteria strain and expand the culture: use an ino...

Embodiment 1

[0040] Embodiment 1. preparation of fermentation broth

[0041] (1) Preparation of saccharification liquid, using double-enzyme hydrolysis method: Weigh 1600g corn flour and add water to 8000mL, adjust the pH value to 6.0-6.4, add a-amylase (600U / g), CaCl 2 (0.01mol / L), maintain at 90°C for about 40 minutes until no starch is detected by iodine solution. Adjust the pH of the liquefied solution to 4.0-4.5, the temperature is 58-62°C, and add glucoamylase (200U / g) to maintain the temperature for 1 hour. Finally, remove the residue with a filter, and use the filtrate to measure the glucose concentration for later use. The glucose concentration of the prepared saccharification solution is 180 g / L.

[0042] (2) Preparation of soybean meal hydrolyzate: weigh a certain amount of soybean meal, add 5 times the amount of water, and mix evenly. Add concentrated sulfuric acid according to 2% of the water amount, mix quickly and evenly, and avoid partial carbonization of the material. A...

Embodiment 2

[0044] Embodiment 2 Lactobacillus activation, expanding culture

[0045] Lactobacillus casei used in the examples was purchased from Beijing Institute of Food Fermentation. The strains stored in the solid medium are picked up with an inoculation loop and inserted into the seed medium, cultured at 42°C for 24 hours, inserted into test tubes and flasks at a ratio of 1:10 for expansion, cultivated at 42°C for 24 hours, and can be seen A large number of bacterial cells are produced, and the bubbles are violent.

[0046] The composition of the seed medium: glucose 4%, soybean peptone 1%, KH 2 PO 4 0.1%, YeastExtract 0.5%, (NH 4 ) 2 SO 4 0.4%, light CaCO 3 1%, pH 6.0-6.5.

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Abstract

The invention provides a biomembrane-electrodialysis coupling continuous production technology of L-lactic acid. The production technology comprises the following steps: a clear liquid biomembrane column reactor is used to perform continuous anaerobic fermentation, the lactic acid producing strains are adsorbed by combined perforated ring filler, an ultrafiltration membrane is used to filter the fermentation liquor, thalli are blocked by the ultrafiltration membrane, the blocked thalli and liquid are sent back to the biomembrane column reactor, and L-lactic acid is separated through electrodialysis. The method provided by the invention uses the biomembrane column reactor to adsorb lactic acid producing strains on the membrane and uses the ultrafiltration membrane to block lactic acid bacteria, thus the thalli concentration and sugar utilization rate can be effectively increased, and the lactic acid concentration, the acid producing rate and the conversation rate from glucose to lacticacid can be increased greatly.

Description

technical field [0001] The invention relates to a production process of L-lactic acid, specifically, a production method for continuously producing L-lactic acid by coupling biofilm fermentation and electrodialysis. Background technique [0002] At present, the method of producing lactic acid at home and abroad is mainly fermentation. The strains include bacteria, Rhizopus oryzae, etc. The fermentation method is mostly batch fermentation, adding CaCO 3 Regulating the pH of the fermentation process, CaCO 3 It brings a lot of trouble for the neutralizer to the post-extraction, and the yield of lactic acid is about 45%. Other neutralizers are Ca(OH) 2 , NH 4 OH, NaOH, etc. In addition, fermenting with Rhizopus oryzae is an aerobic fermentation with high energy consumption and low yield. [0003] Since Lactobacillus can be fermented anaerobically, it is suitable for continuous production. Continuous fermentation simplifies many unit operations, improves the utilization ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C07C59/08C07C51/42C12R1/07C12R1/225C12R1/245
Inventor 唐开宇高大成
Owner CHINA PETROLEUM & CHEM CORP
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