Nutritive salt solution and application thereof

A nutrient salt and solution technology, which is applied to nutrient salt solution and its application field, can solve the problems of short fermentation period, low acid production rate, low acid yield and the like, so as to increase acid yield, improve acid production rate and yield. added effect

Pending Publication Date: 2019-06-11
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of short fermentation period, low acid production rate, and low acid yield in the method for producing long-chain dibasic acids by fermentation in the prior art, the invention provides a nutrient salt solution and its use in the production of long-chain dibasic acids by fermentation. Application of dibasic acid method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Seed medium: 3.0% sucrose, 0.60% corn steep liquor, 1.0% yeast extract, KH 2 PO 4 1.2%, urea 0.30%.

[0059] Fermentation medium: glucose 3.0%, corn steep liquor 0.60%, yeast extract 0.30%, potassium nitrate 1.1%, potassium dihydrogen phosphate 0.80%, urea 0.20%, ammonium sulfate 0.15% and sodium chloride 0.10%.

[0060] There are two kinds of feed medium: one is carbasundecane; the other is a solution containing nutrient salt (glucose 0.2%, yeast extract 0.1%, corn steep liquor 0.15%, potassium dihydrogen phosphate 0.012%, urea 0.015%, The rest is water; percentage is mass volume ratio, g / 100mL).

[0061] The shake flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 50mL), the initial pH is 6.3, and culture at 29°C and 220rpm shaker for 1.5 days .

[0062] The seed tank culture process is to get the shake flask seeds and insert them in the 10L seed tan...

Embodiment 2

[0075] Seed medium: 1% sucrose, 0.30% corn steep liquor, 0.6% yeast extract, KH 2 PO 4 0.8%, urea 0.25%.

[0076] Fermentation medium: 2.0% glucose, 0.20% corn steep liquor, 0.25% yeast extract, 0.05% potassium nitrate, 0.07% potassium dihydrogen phosphate, 0.15% urea, 0.20% ammonium sulfate and 0.12% sodium chloride.

[0077] There are two kinds of feeding medium: one is carbon dodecane; the other is a solution containing nutrient salt (yeast extract 0.1%, ammonium sulfate 0.05%, corn steep liquor 0.05%, potassium dihydrogen phosphate 0.008%, urea 0.01% , and the rest is water; the percentage is mass-volume ratio, g / 100mL).

[0078] The shaking flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 60mL), the initial pH is 6.5, and culture at 32°C and 250rpm shaker for 1 day .

[0079] The seed tank culture process is to get the shake flask seeds and insert them...

Embodiment 3

[0092] Seed medium: 3% sucrose, 1% corn steep liquor, 0.2% yeast extract, KH 2 PO 4 0.9%, urea 0.5%.

[0093] Fermentation medium: glucose 5%, corn steep liquor 0.7%, yeast extract 0.3%, potassium nitrate 0.55%, potassium dihydrogen phosphate 0.60%, urea 0.20%, ammonium sulfate 0.20% and sodium chloride 0.15%.

[0094] There are two kinds of feeding medium: one is carbon tridecane; the other is a solution containing nutrient salt (0.5% glucose, 0.15% ammonium sulfate, 0.10% corn steep liquor, 0.008% potassium dihydrogen phosphate, 0.007% urea, The rest is water; percentage is mass volume ratio, g / 100mL).

[0095] The shake flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 100mL), the initial pH is 6.0, and culture at 200rpm shaker at 30°C for 2 days .

[0096] The seed tank cultivation process is to get the shake flask seeds and insert them in the 10L seed t...

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PUM

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Abstract

The invention discloses nutritive salt and an application thereof to a method for producing long-chain binary acid through continuous fermentation. A solution containing nutritive salt comprises the following components of 0.0%-1.0% of glucose, 0.05-0.15% of corn steep liquor, 0.0%-0.3% of yeast cream, 0.005%-0.015% of potassium dihydrogen phosphate, 0.005%-0.015% of urea, 0.01%-0.15% of ammoniumsulphate and the balance water. The continuous fermentation method comprises the steps of performing fermentation for 100-150h, performing continuous flow feeding on substrates, and besides, performing continuous flow feeding on the solution containing nutritive salt. According to the nutritive salt solution disclosed by the invention, the acid production rate of long-chain binary acid can be increased by a fermentation method, and the acid yield can be substantially increased. According to the method disclosed by the invention, on the base that no equipment is additionally arranged, the long-term activity of thalli can be guaranteed, so that sustained production of the long-chain binary acid can be guaranteed, the yield of the long-chain binary acid can be substantially increased, and theyield is also increased.

Description

technical field [0001] The invention relates to a nutrient salt solution and its application in a method for fermenting and producing long-chain dibasic acids. Background technique [0002] Long-chain dibasic acid (LDCA, HOOC (CH 2 )nCOOH, n≥7) has a wide range of applications. It can be used as a raw material to synthesize special nylon (polyamide), high-grade spices, high-grade hot-melt adhesives, cold-resistant plasticizers, high-grade lubricating oils, high-grade antirust agents, high-grade paints and coatings Wait. [0003] Generally speaking, long-chain dibasic acids can be produced by chemical synthesis or biological fermentation. The chemical synthesis method has a long synthetic route and strict reaction conditions. It needs to be carried out under high temperature and high pressure conditions, and the catalyst requirements are relatively strict. Therefore, on an industrial scale, there are few long-chain dibasic acids synthesized by chemical methods, only dodecan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/44C12R1/74
Inventor 路飞徐敏刘修才
Owner CATHAY R&D CENT CO LTD
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