Nutritive salt solution and application thereof
A nutrient salt and solution technology, which is applied to nutrient salt solution and its application field, can solve the problems of short fermentation period, low acid production rate, low acid yield and the like, so as to increase acid yield, improve acid production rate and yield. added effect
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Embodiment 1
[0058] Seed medium: 3.0% sucrose, 0.60% corn steep liquor, 1.0% yeast extract, KH 2 PO 4 1.2%, urea 0.30%.
[0059] Fermentation medium: glucose 3.0%, corn steep liquor 0.60%, yeast extract 0.30%, potassium nitrate 1.1%, potassium dihydrogen phosphate 0.80%, urea 0.20%, ammonium sulfate 0.15% and sodium chloride 0.10%.
[0060] There are two kinds of feed medium: one is carbasundecane; the other is a solution containing nutrient salt (glucose 0.2%, yeast extract 0.1%, corn steep liquor 0.15%, potassium dihydrogen phosphate 0.012%, urea 0.015%, The rest is water; percentage is mass volume ratio, g / 100mL).
[0061] The shake flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 50mL), the initial pH is 6.3, and culture at 29°C and 220rpm shaker for 1.5 days .
[0062] The seed tank culture process is to get the shake flask seeds and insert them in the 10L seed tan...
Embodiment 2
[0075] Seed medium: 1% sucrose, 0.30% corn steep liquor, 0.6% yeast extract, KH 2 PO 4 0.8%, urea 0.25%.
[0076] Fermentation medium: 2.0% glucose, 0.20% corn steep liquor, 0.25% yeast extract, 0.05% potassium nitrate, 0.07% potassium dihydrogen phosphate, 0.15% urea, 0.20% ammonium sulfate and 0.12% sodium chloride.
[0077] There are two kinds of feeding medium: one is carbon dodecane; the other is a solution containing nutrient salt (yeast extract 0.1%, ammonium sulfate 0.05%, corn steep liquor 0.05%, potassium dihydrogen phosphate 0.008%, urea 0.01% , and the rest is water; the percentage is mass-volume ratio, g / 100mL).
[0078] The shaking flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 60mL), the initial pH is 6.5, and culture at 32°C and 250rpm shaker for 1 day .
[0079] The seed tank culture process is to get the shake flask seeds and insert them...
Embodiment 3
[0092] Seed medium: 3% sucrose, 1% corn steep liquor, 0.2% yeast extract, KH 2 PO 4 0.9%, urea 0.5%.
[0093] Fermentation medium: glucose 5%, corn steep liquor 0.7%, yeast extract 0.3%, potassium nitrate 0.55%, potassium dihydrogen phosphate 0.60%, urea 0.20%, ammonium sulfate 0.20% and sodium chloride 0.15%.
[0094] There are two kinds of feeding medium: one is carbon tridecane; the other is a solution containing nutrient salt (0.5% glucose, 0.15% ammonium sulfate, 0.10% corn steep liquor, 0.008% potassium dihydrogen phosphate, 0.007% urea, The rest is water; percentage is mass volume ratio, g / 100mL).
[0095] The shake flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 100mL), the initial pH is 6.0, and culture at 200rpm shaker at 30°C for 2 days .
[0096] The seed tank cultivation process is to get the shake flask seeds and insert them in the 10L seed t...
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