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Tissue culture method of dwarf radix ophiopogonis

A dwarf Ophiopogon japonicus and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of low survival or germination rate, achieve easy reproduction, shorten the reproduction cycle, and solve the effects of slow sowing and reproduction

Active Publication Date: 2015-05-13
中林绿苑江苏生态环境有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The propagation method of dwarf Ophiopogon japonicus is mainly by division, which can be carried out in all seasons, and can also be sown, but the survival or germination rate is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for tissue culture of dwarf Ophiopogon japonicus, comprising the following steps:

[0021] Step 1, disinfection and sterilization:

[0022] Select young leaves that have just germinated that year, rinse them with tap water, soak them in 70% alcohol for 15 seconds on an ultra-clean workbench, then sterilize them in 0.1% mercuric solution for 1 minute, pour off the sterilizing solution, and use the pre- Rinse the prepared sterile water 4 times, drain the water, and inoculate the sterilized leaves on the germination medium;

[0023] Step 2, primary culture:

[0024] Inoculate the sterilized leaves on MS+BA 0.5mg / L+NAA0.5mg / L+KT1.0mg / L+sucrose 30g / L+agar 5g / L medium, the pH value of the medium is 5.6, and the MS medium passes through 1.1kg / cm 2 Autoclave for 20 minutes, and cultivate in the dark at a temperature of 25±2°C for about 20 days. The leaves begin to callus. By the 30th day, most of the leaves have formed callus. Cut off the callus and enter the next st...

Embodiment 2

[0034] A method for tissue culture of dwarf Ophiopogon japonicus, comprising the following steps:

[0035] Step 1, disinfection and sterilization:

[0036] Select young leaves that have just germinated that year, rinse them with tap water, soak them in 70% alcohol for 15 seconds on an ultra-clean workbench, then sterilize them in 0.1% mercuric solution for 3 minutes, pour off the sterilizing solution, and use Rinse the prepared sterile water 5 times, drain the water, and inoculate the sterilized leaves on the germination medium;

[0037] Step 2, primary culture:

[0038] Inoculate the sterilized leaves on MS+BA 0.5mg / L+NAA0.5mg / L+KT1.0mg / L+sucrose 30g / L+agar 7g / L medium, the pH value of the medium is 5.8, and the MS medium is passed through 1.1kg / cm 2 Autoclave for 20 minutes, and cultivate in the dark at a temperature of 25±2°C for about 20 days. The leaves begin to callus. By the 33rd day, most of the leaves have formed callus. Cut off the callus and enter the next stage o...

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PUM

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Abstract

The invention discloses a tissue culture method of dwarf radix ophiopogonis. The tissue culture method of the dwarf radix ophiopogonis comprises: disinfection and sterilization, initial culture, subculture, rooting culture and acclimatization and transplant. One-year dwarf radix ophiopogonis tender leaves as explants are aseptically inoculated onto an inducing bud medium to generate callus. The generated callus is adopted for the subculture, and then induced plantlets are inoculated onto a strong seedling medium for strong seedling culture. The induced plantlets are inoculated onto a rooting medium for rooting induction to generate rooting sprouts. Regeneration plants are obtained through acclimatization and domestication of the rooting sprouts. Through use of the tissue culture method, breeding of the dwarf radix ophiopogonis is easier, the breeding cycle is shortened, the survival rate or germination rate of the dwarf radix ophiopogonis can be improved, and the problems of slow sowing and breeding and low survival rate of division plants can be solved.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for tissue culture of dwarf Ophiopogon japonicus. Background technique [0002] Dwarf Ophiopogon japonicus is a perennial dwarf evergreen herb of Liliaceae. The leaves are clustered, the leaves are short and the plants are short, shade-loving, cold-tolerant, regardless of soil, and strong adaptability. The dwarf Ophiopogon japonicus is resistant to low temperatures below -18.9 degrees, and the plant height is only 10-15 cm. It likes warm, humid and shaded places, and can grow well in northern regions. The ground cover formed by the dwarf Ophiopogon japonicus is neat, the landscape effect is excellent, the management is labor-saving, and no pruning is required. It is an excellent evergreen lawn plant. [0003] The propagation method of dwarf Ophiopogon japonicus is mainly by division, which can be carried out in all seasons, and can also be sown, but the survival or ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王海珍史云光史清云张荣良蔡亚萍朱艳
Owner 中林绿苑江苏生态环境有限公司