Preparation of tetracyclic triterpene compound and anti-aging application
A technology of tetracyclic triterpenes and compounds, which is applied in the field of medicine and food of compounds, can solve the problems of reducing the quality of life of patients and the heavy economic burden of family members, and achieve the effect of prolonging the replicative life span
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Embodiment 1
[0029] Embodiment 1 Preparation of compound A and compound B
[0030] Obtain the preparation method of compound A and compound B from motherwort of traditional Chinese medicine, concrete steps are:
[0031] (1) Grinding and extraction: 300 g of motherwort, a traditional Chinese medicine, was crushed, and extracted with 1 L of methanol (industrial grade) at room temperature for 5 days (shaking). After suction filtration and concentration, a crude sample of the methanol extract was obtained, and the solvent was partitioned with water and ethyl acetate to obtain a total of 6 grams of a crude sample of the ethyl acetate layer and 14 grams of a crude sample of the aqueous layer.
[0032] (2) Separation and purification: the ethyl acetate layer crude sample is separated through a silica gel open column (200-300 mesh, the following is the solvent volume ratio, n-hexane: chloroform=2: 8, 1: 9, 0: 10, chloroform : Methanol=98:2, 96:4, 94:6, 92:8, 90:10), respectively collect and combi...
Embodiment 2
[0036] Embodiment 2 Qualitative identification of the physical and chemical characteristics and compound structure of compound A, B obtained in embodiment 1
[0037] The chemical structures of compounds A and B were determined by mass spectrometry (including high-resolution mass spectrometry) and nuclear magnetic resonance.
[0038] Compound A, pale yellow in color, is easily soluble in methanol. When analyzed by TLC, using chloroform:methanol (8:2) as developing solvent, on a positive silica plate with a height of 5cm, the R f 0.5 of the value. This sample has significant UV absorption at 228nm.
[0039] Compound B, pale yellow in color, is easily soluble in methanol. When analyzed by TLC, using chloroform:methanol (9:1) as a developing solvent, on a 5cm positive silica plate, its R f 0.5 of the value. This sample has significant UV absorption at 228nm.
[0040] Compound A, the molecular formula is C 36 h 56 o 11 ; HRFT-ICR MS: m / z 687.3703 [M+Na] + , theoretical va...
Embodiment 3
[0042] Example 3 Compound B extends the replicative lifespan of yeast cells
[0043] Compound B significantly prolongs the replicative lifespan of yeast cells in an anti-aging in vitro screening model.
[0044] Saccharomyces cerevisiae is a single-celled eukaryote that has been widely used in recent years to study the aging process. Yeast has many advantages in anti-aging research. It is the first eukaryotic cell organism with a complete genome, the research period is short, and it has a high degree of homology with mammals. There are two life cycles of Saccharomyces cerevisiae used in antiaging research: replicative lifespan and temporal lifespan. Replicative lifespan refers to the maximum number of times a yeast cell can divide and proliferate before death, that is, the maximum number of daughter cells that can be produced. Due to the complicated operation of traditional methods, Stefanie, Jarolim and others discovered a special yeast - K6001 - the daughter cells of this s...
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