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Application of pterostilbene in antifungal biofilm medicine

A technology of biofilm and pterostilbene, which is applied in the field of medicine, can solve the problems that antifungal drugs do not have anti-biofilm activity, affect the prognosis of patients, and make clinical treatment difficult.

Inactive Publication Date: 2013-10-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in vivo catheter or biological device-associated fungal infections that grow in the form of biofilms and exhibit high drug resistance pose great difficulties to clinical treatment, forcing clinicians to remove catheters or biodevices from patients while performing antifungal therapy. biodevices, thereby affecting patient outcomes
However, in the prior art, drugs with antifungal activity often do not have antibiofilm activity, resulting in that the expected effect cannot be obtained when it is used to treat fungi that grow in the form of biofilm

Method used

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  • Application of pterostilbene in antifungal biofilm medicine
  • Application of pterostilbene in antifungal biofilm medicine
  • Application of pterostilbene in antifungal biofilm medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: the effect of pterostilbene alone on the biofilm of different Candida albicans strains

[0075] Materials and methods

[0076] 1. Test drug

[0077] Pterostilbene: provided by Guangzhou Chemical Co., Ltd., Chinese Academy of Sciences.

[0078] Fluconazole: purchased from Sigma Company.

[0079] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0080] XTT: purchased from Sigma Corporation.

[0081] Menadione (menadione): purchased from Sigma Company.

[0082] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0083] Acetone: purchased from Sigma Company.

[0084] Pterostilbene was made into a 6.4 mg / ml solution with dimethyl sulfoxide, amphotericin B was made into a 4 mg / ml solution with dimethyl sulfoxide, and fluconazole was made into a 102.4 mg / ml solution with dimethyl sulfoxide. Store at -20°C. Before the experiment, the drug stock solution was taken out and placed...

Embodiment 2

[0116] Embodiment 2: The effect of pterostilbene alone on the biofilm of different Cryptococcus neoformans strains

[0117] Materials and methods

[0118] 1. Test drug

[0119] Pterostilbene: provided by Guangzhou Chemical Co., Ltd., Chinese Academy of Sciences.

[0120] Fluconazole: purchased from Sigma Company.

[0121] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0122] XTT: purchased from Sigma Corporation.

[0123] Menadione (menadione): purchased from Sigma Company.

[0124] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0125] Acetone: purchased from Sigma Company.

[0126] Pterostilbene was made into 6.4 mg / ml with dimethyl sulfoxide, amphotericin B was made into 4 mg / ml with dimethyl sulfoxide, and fluconazole was made into 102.4 mg / ml with dimethyl sulfoxide. °C storage. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, ...

Embodiment 3

[0158] Embodiment 3: Pterostilbene single use is to the effect of different Aspergillus strain biofilms

[0159] Materials and methods

[0160] 1. Test drug

[0161] Pterostilbene: provided by Guangzhou Chemical Co., Ltd., Chinese Academy of Sciences.

[0162] Fluconazole: purchased from Sigma Company.

[0163] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0164] XTT: purchased from Sigma Corporation.

[0165] Menadione (menadione): purchased from Sigma Company.

[0166] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0167] Acetone: purchased from Sigma Company.

[0168] Pterostilbene was made into 6.4 mg / ml with dimethyl sulfoxide, amphotericin B was made into 4 mg / ml with dimethyl sulfoxide, and fluconazole was made into 102.4 mg / ml with dimethyl sulfoxide. °C storage. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed...

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Abstract

The invention discloses new application of the pterostilbene to the anti-fungal biofilm and relates to the application of the pterostilbene to the anti-fungal biofilm therapeutic. The pterostilbene as a compound has the follow chemical structural formula. The experiments prove that the pterostilbene has good anti-fungal biofilm activity in vitro. The experimental results prove that the pterostilbene can effectively inhibit the growth and the proliferation of the fungal biofilm in vitro and can effectively inhibit the mature biofilm.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a new application of pterostilbene in antifungal biofilms. Background technique [0002] Pterostilbene is derived from the antifungal active ingredient in dried blood products. It belongs to polyhydroxy stilbene compounds and is a homologue of resveratrol. Its structure is shown in formula (I). It is a white crystalline powder and is sensitive to air. , with a melting point of 89-92°C, has rich medicinal value, and has a certain effect on the treatment of cancer, hypertension, and hyperlipidemia. Tu Pengfei, Peking University Modern Research Center of Traditional Chinese Medicine, etc. have reported on the antifungal effect of pterostilbene, (Hu Yingqing, Tu Pengfei, Research on stilbene compounds and their antifungal activities in Dracaena glabrata, Chinese Herbal Medicine, 2001, 32 (2): 104 -105). [0003] [0004] Biofilm is a microbial aggregation group, which is a microe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/09A61P31/10A01N31/16A01P3/00
Inventor 王彦赵兰雪李德东邹永古金华曹永兵姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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