Natural product preparation capable of reducing methane production amount of rumen microorganisms

A technology of rumen microorganisms and natural products, applied in the field of natural product preparations, can solve the problems of chemical substance tolerance residues and limitations in practical applications, etc.

Inactive Publication Date: 2013-11-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] ionophores (monensin, Van Nevel and Demeyer et al., 1995), organic acids (fumaric acid, Yuan et al., 2007), halogenated compounds (bromo Chloromethane, Denman et al. 2007; Sodium 2-bromoethanesulfonate, Guo et al., 2008) and other chemicals can reduce methane emissions, but their practical application is limited due to the tolerance and residue problems of chemicals

Method used

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  • Natural product preparation capable of reducing methane production amount of rumen microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Grind Vitex fruit into powder with a traditional Chinese medicine grinder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 70% ethanol solution;

[0024] (2) Vortex → 35rpm rotary mixer mixing for 30min → vortex →rotary mixer mixing for 30min (twice total 1h) → 3000r centrifugation for 5min;

[0025] (3) Draw the supernatant to a freezing bottle, and dry it with a nitrogen blower;

[0026] (4) Retain the precipitate in the centrifuge tube, continue to add 5mL 70% ethanol→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min (three times a total of 1.5h)→3000r Centrifuge for 5min;

[0027] (5) Take the supernatant to the blown-dried freezing bottle, blow dry with nitrogen blower, freeze dryer for 24-48 hours, wait until it rises to room temperature, weigh, seal the bottle, and store at -20°C.

Embodiment 2

[0029] (1) Grind Vitex fruit into powder with a traditional Chinese medicine grinder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 60% ethanol solution;

[0030] (2) Vortex → 35rpm rotary mixer mixing for 30min → vortex →rotary mixer mixing for 30min (twice total 1h) → 3000r centrifugation for 5min;

[0031] (3) Draw the supernatant to a freezing bottle, and dry it with a nitrogen blower;

[0032] (4) Precipitate remains in the centrifuge tube, continue to add 5mL 60% ethanol→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min (three times a total of 1.5h)→3000r Centrifuge for 5min;

[0033] (5) Take the supernatant to the blown-dried freezing bottle, blow dry with nitrogen blower, freeze dryer for 24-48 hours, wait until it rises to room temperature, weigh, seal the bottle, and store at -20°C.

Embodiment 3

[0035] (1) Grind Vitex fruit into powder with a traditional Chinese medicine grinder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 80% ethanol solution;

[0036] (2) Vortex → 35rpm rotary mixer mixing for 30min → vortex →rotary mixer mixing for 30min (twice total 1h) → 3000r centrifugation for 5min;

[0037] (3) Draw the supernatant to a freezing bottle, and dry it with a nitrogen blower;

[0038] (4) Keep the precipitate in the centrifuge tube, continue to add 5mL 80% ethanol→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min (three times a total of 1.5h)→3000r Centrifuge for 5min;

[0039] (5) Take the supernatant to the blown-dried freezing bottle, blow dry with nitrogen blower, freeze dryer for 24-48 hours, wait until it rises to room temperature, weigh, seal the bottle, and store at -20°C.

[0040] Combined with the in vitro fermentation test in the rumen, the extent to which the preparation reduces ...

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Abstract

The invention relates to the field of rumen regulation, in particular to a natural product preparation capable of reducing methane production amount of rumen microorganisms. The natural production preparation is dried powder prepared by smashing fruits of fructus viticis, extracting the smashed fruits through ethanol solution with weight percentage of 60%-80% and performing nitrogen blowing, freezing and drying on the extracted product. The natural product preparation can obviously reduce the methane production amount of the rumen microorganisms. The invention further provides a preparation process of the natural product preparation and an application of the natural product preparation for reducing the methane production amount of the rumen microorganisms. Effective components like volatile oil of the fructus viticis can be extracted through ethanol, and the natural product preparation which is dried powder can be prepared through nitrogen blowing, freezing and drying. According to in-vitro fermentation test of rumens, 0.4g/L of the preparation, namely adding 40mg of the natural product preparation in each gram of dry matter, the methane production amount of the rumen microorganisms can be effectively reduced to 21.7%.

Description

technical field [0001] The invention relates to the field of rumen regulation, in particular to a natural product preparation for reducing the methane production of rumen microorganisms. Background technique [0002] Roughage is the main source of feed for ruminants, and the degradation of various fibrous substances in roughage is undoubtedly the most important digestive process that occurs in the rumen. This process produces a large amount of hydrogen. If hydrogen accumulates, it will inhibit the hydrogenation of reduced nicotinamide adenine dinucleotide, resulting in the accumulation of reductive fermentation products such as lactic acid (Wolin et al., 1997), which in turn hinders the digestion of roughage and beneficial microorganisms. growth is hindered. The generation of methane is the main way of hydrogen excretion in the rumen. During the ruminal metabolism of ruminants, about 2%-15% of the feed can be lost in the form of methane, depending on the feeding level, die...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/14A23K1/18
CPCY02P60/22
Inventor 王佳堃刘美刘建新
Owner ZHEJIANG UNIV
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