Natural product preparation capable of reducing methane production amount of rumen microorganisms
A technology of rumen microorganisms and natural products, applied in the field of natural product preparations, can solve the problems of chemical substance tolerance residues and limitations in practical applications, etc.
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Embodiment 1
[0023] (1) Grind Vitex fruit into powder with a traditional Chinese medicine grinder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 70% ethanol solution;
[0024] (2) Vortex → 35rpm rotary mixer mixing for 30min → vortex →rotary mixer mixing for 30min (twice total 1h) → 3000r centrifugation for 5min;
[0025] (3) Draw the supernatant to a freezing bottle, and dry it with a nitrogen blower;
[0026] (4) Retain the precipitate in the centrifuge tube, continue to add 5mL 70% ethanol→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min (three times a total of 1.5h)→3000r Centrifuge for 5min;
[0027] (5) Take the supernatant to the blown-dried freezing bottle, blow dry with nitrogen blower, freeze dryer for 24-48 hours, wait until it rises to room temperature, weigh, seal the bottle, and store at -20°C.
Embodiment 2
[0029] (1) Grind Vitex fruit into powder with a traditional Chinese medicine grinder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 60% ethanol solution;
[0030] (2) Vortex → 35rpm rotary mixer mixing for 30min → vortex →rotary mixer mixing for 30min (twice total 1h) → 3000r centrifugation for 5min;
[0031] (3) Draw the supernatant to a freezing bottle, and dry it with a nitrogen blower;
[0032] (4) Precipitate remains in the centrifuge tube, continue to add 5mL 60% ethanol→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min (three times a total of 1.5h)→3000r Centrifuge for 5min;
[0033] (5) Take the supernatant to the blown-dried freezing bottle, blow dry with nitrogen blower, freeze dryer for 24-48 hours, wait until it rises to room temperature, weigh, seal the bottle, and store at -20°C.
Embodiment 3
[0035] (1) Grind Vitex fruit into powder with a traditional Chinese medicine grinder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 80% ethanol solution;
[0036] (2) Vortex → 35rpm rotary mixer mixing for 30min → vortex →rotary mixer mixing for 30min (twice total 1h) → 3000r centrifugation for 5min;
[0037] (3) Draw the supernatant to a freezing bottle, and dry it with a nitrogen blower;
[0038] (4) Keep the precipitate in the centrifuge tube, continue to add 5mL 80% ethanol→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min→vortex shaker→rotate mixer for 30min (three times a total of 1.5h)→3000r Centrifuge for 5min;
[0039] (5) Take the supernatant to the blown-dried freezing bottle, blow dry with nitrogen blower, freeze dryer for 24-48 hours, wait until it rises to room temperature, weigh, seal the bottle, and store at -20°C.
[0040] Combined with the in vitro fermentation test in the rumen, the extent to which the preparation reduces ...
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Abstract
Description
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