Identification method of channel catfish species

Abstract

The invention relates to an identification method of channel catfish species. The identification method comprises following steps: genome DNA of channel catfish muscular tissues is extracted; a PCR primer is designed in mitochondrial DNA D-loop region; the genome DNA is subjected to PCR amplification with the presence of the PCR primer so as to obtain mitochondrial DNA D-loop sequences; the mitochondrial DNA D-loop sequences are subjected to enzyme digestion for 2 to 3h at a temperature of 37 DEG C by using restriction enzyme EcoR I; and then the mitochondrial DNA D-loop sequences are subjected to agarose gel electrophoresis after enzyme digestion, and the channel catfish species are identified according to the results of electrophoresis. Enzyme sites are searched based on sequencing of D-loop, and enzyme digestion identification is performed, so that sequencing is not necessary for identification of channel catfish species. The identification method is capable of identifying channel catfish species at a molecular level; operation is simple and easy; consumed time is short; and identification method is suitable for species identification of channel catfish in import and export trade.

Description

Technical field [0001] The invention relates to a method for identifying channel catfish species, in particular to a method for identifying the genetic information of channel catfish species using a mitochondrial DNA D-loop control region, and belongs to the field of molecular biology PCR genetic identification. Background technique [0002] Channel catfish (Ictalurus punctatus), also known as Channel Catfish, belongs to Siluriformes (Siluriformes), Ictaluridae (Ictaluridae), Ictalurus (Ictalurus). It is native to North America, and its natural distribution areas are in the central United States, southern Canada and northern Mexico. Channel catfish is a valuable freshwater economic fish that is deeply loved by consumers in the United States, Canada and many other countries. The processed finished or semi-finished products are very popular in markets such as the United States, Japan, Europe and Canada, and the market demand is huge. Since 1984, the Hubei Provincial Fisheries Res...

AI Technical Summary

Problems solved by technology

The results of relevant scientific and technological novelty searches show that there are reports abroad on the PCR-RFLP data of mitochondrial DNA for species identification in cod, flounder and other products, but there is no molecular data of the D-loop region of mitochondrial DNA for channel catfish species identification. report

Moreover, the PCR-RFLP method is cumbersome to operate, and it is mostly used for genetic polymorphism analysis of different populations of the same species, which is not suitable for rapid and economical identification of channel catfish species.

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