Identification method of channel catfish species

A technology for channel catfish and identification methods, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of cumbersome operation process, lack of mitochondrial DNA molecular data, unsuitable for fast and economical identification of channel catfish species, etc. , to achieve the effect of short time and simple operation

Inactive Publication Date: 2014-08-20
罗志萍
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of relevant scientific and technological novelty searches show that there are reports abroad on the PCR-RFLP data of mitochondrial DNA for species identification in cod, flounder and other products, but there is no molecular data of the D-loop region of mitochondrial DNA for channel catfish species identification. report
Moreover, the PCR-RFLP method is cumbersome to operate, and it is mostly used for genetic polymorphism analysis of different populations of the same species, which is not suitable for rapid and economical identification of channel catfish species.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Identification method of channel catfish species
  • Identification method of channel catfish species
  • Identification method of channel catfish species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] The principles and features of the present invention will be described below with reference to the accompanying drawings. The examples cited are only used to explain the present invention, and are not used to limit the scope of the present invention.

[0043] 1. Extraction of genomic DNA

[0044] The conventional phenol-chloroform extraction method is adopted. For specific reference, the method of Sambrook (2002) is slightly modified:

[0045] (1) Take about 0.1g of the tail fin fixed with alcohol in a 2ml centrifuge tube, cut into pieces, and dry at 37°C;

[0046] (2) Add 500ul extraction buffer to the centrifuge tube, and add RNaseA to a final concentration of 20μg / ml, and water bath at 37°C for 1h. Then add proteinase K to the extraction buffer to a final concentration of 200μg / ml, and bath at 55°C overnight;

[0047] (3) After cooling the digested sample to room temperature, add an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1), gently invert it upside down for ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an identification method of channel catfish species. The identification method comprises following steps: genome DNA of channel catfish muscular tissues is extracted; a PCR primer is designed in mitochondrial DNA D-loop region; the genome DNA is subjected to PCR amplification with the presence of the PCR primer so as to obtain mitochondrial DNA D-loop sequences; the mitochondrial DNA D-loop sequences are subjected to enzyme digestion for 2 to 3h at a temperature of 37 DEG C by using restriction enzyme EcoR I; and then the mitochondrial DNA D-loop sequences are subjected to agarose gel electrophoresis after enzyme digestion, and the channel catfish species are identified according to the results of electrophoresis. Enzyme sites are searched based on sequencing of D-loop, and enzyme digestion identification is performed, so that sequencing is not necessary for identification of channel catfish species. The identification method is capable of identifying channel catfish species at a molecular level; operation is simple and easy; consumed time is short; and identification method is suitable for species identification of channel catfish in import and export trade.

Description

Technical field [0001] The invention relates to a method for identifying channel catfish species, in particular to a method for identifying the genetic information of channel catfish species using a mitochondrial DNA D-loop control region, and belongs to the field of molecular biology PCR genetic identification. Background technique [0002] Channel catfish (Ictalurus punctatus), also known as Channel Catfish, belongs to Siluriformes (Siluriformes), Ictaluridae (Ictaluridae), Ictalurus (Ictalurus). It is native to North America, and its natural distribution areas are in the central United States, southern Canada and northern Mexico. Channel catfish is a valuable freshwater economic fish that is deeply loved by consumers in the United States, Canada and many other countries. The processed finished or semi-finished products are very popular in markets such as the United States, Japan, Europe and Canada, and the market demand is huge. Since 1984, the Hubei Provincial Fisheries Res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 罗志萍王华全肖武汉
Owner 罗志萍
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap