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A kind of preparation method and application of crocodile skin collagen peptide

A skin collagen and protein peptide technology, applied in skin care preparations, animal/human proteins, specific peptides, etc., can solve the problems of lack of secondary utilization of scraps, incomplete development, environmental pollution, etc., and achieve good social benefits and The effect of economic benefits, increasing added value and reducing environmental pollution

Active Publication Date: 2015-09-23
GUANGZHOU TUOLONG BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, collagen peptides using crocodiles as raw materials are rarely reported.
The research on crocodile skin is still at the primary level of making leather goods and handicrafts, and its development is not yet complete, and some leftovers also lack opportunities for secondary use
At present, the development of crocodile skin is mainly concentrated on the production of leather goods, and the leftovers produced in the processing process and some crocodile skins specially used for eating crocodile meat cannot be used for handicraft processing. If these leftovers are not effectively utilized, they will become Waste not only causes great waste, but also causes environmental pollution

Method used

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  • A kind of preparation method and application of crocodile skin collagen peptide
  • A kind of preparation method and application of crocodile skin collagen peptide
  • A kind of preparation method and application of crocodile skin collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Take 100g of fresh crocodile skin, scrape off the fat, wash it, cut it into square pieces with a side length of about 2 cm, add 1L of distilled water, cook in an electric rice cooker for 30 minutes, cool to room temperature, and mash. Add 4% alkaline protease (alkaline protease derived from Bacillus licheniformis, Zaozhuang Jienuo Biological Enzyme Co., Ltd.) based on the weight of crocodile skin, after hydrolysis at pH 11, temperature 40 ° C for 3 hours, heat and boil for 10 minutes to make the enzyme Inactivate. Centrifuge at room temperature at 4000 rpm for 20 minutes, take the supernatant, and freeze-dry to obtain crocodile skin collagen peptide powder.

[0044] Figure 4 The MALDI-TOF MS spectrum of the crocodile skin collagen peptide obtained in Example 1 is shown. The molecular weight of the crocodile skin collagen peptide powder that embodiment 1 obtains is 1.2KD. The molecular weight is small, indicating that the enzymatic hydrolysis is sufficient during the...

Embodiment 2

[0046] Take 500g of fresh crocodile skin, scrape off the fat, wash it, cut it into small pieces, add 5L of distilled water, cook in an electric rice cooker for 30 minutes, cool to room temperature, and mash. Add 4% alkaline protease (alkaline protease derived from Bacillus licheniformis, Zaozhuang Jienuo Biological Enzyme Co., Ltd.) based on the weight of crocodile skin, after hydrolysis at pH 11, temperature 40 ° C for 3 hours, heat and boil for 10 minutes to make the enzyme Inactivate. Then centrifuge at 4000 rpm for 20 minutes at room temperature, take the supernatant, and spray dry to obtain crocodile skin collagen peptide powder.

[0047] The molecular weight of the obtained crocodile skin collagen peptide powder is 1.2KD. The molecular weight is small, indicating that the enzymatic hydrolysis is sufficient during the treatment with alkaline protease. The content of hydroxyproline was measured to be 6.2 μg / mg. The aqueous solution of crocodile skin collagen peptide pow...

Embodiment 3

[0048] Embodiment 3: the in vitro moisturizing effect of the crocodile skin collagen peptide of the present application

[0049] According to the difference in moisturizing properties of humectants, different humectants have different forces on water molecules, and the ability to absorb water and retain water is also different. Dry the samples containing a certain amount of moisture in a desiccator with constant temperature and humidity, and regularly weigh the reduction of the sample mass. Through comparative analysis, the moisture retention of different samples can be compared. In order to better simulate the actual situation of the skin, affix 3M adhesive plaster to the slide, and each test substance (respectively distilled water (negative control), 0.3% hyaluronic acid (positive control), crocodile prepared in Example 1) Skin collagen peptide and sharkskin collagen peptide) aqueous solution (solid-liquid ratio is 1: 4) is coated on it, puts in the desiccator that fills s...

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Abstract

A preparation method of crocodile-skin collagen peptide comprises the following steps: boiling fat-free crocodile skin in a solvent; treating the boiled crocodile skin with a protease to obtain an enzymatic hydrolysate; conducting centrifugal separation on the enzymatic hydrolysate; taking supernate and drying, so as to obtain the crocodile-skin collagen peptide. The crocodile-skin collagen peptide obtained according to the prepartion method can be used as an antioxidant, a humectant, an anti-DNA damage agent, or a whitening additive in cosmetics. The preparation method is simple and convenient, is suitable for mass production, not only can improve the development added value of a crocodile product, but also can reduce environmental pollution, brings high economic value and social value.

Description

technical field [0001] The present application relates to the field of biological products, in particular to a preparation method and application of crocodile skin collagen peptide. Background technique [0002] Collagen is the most abundant type of protein in the human body or other animals, accounting for 25-33% of the total protein content. Collagen is the main component of extracellular matrix (ECM), which plays an important role in maintaining the elasticity of skin and blood vessel walls, keeping hair and nails soft and shiny, and improving the lubricity of cartilage. Due to its unique physical and chemical properties and excellent biocompatibility, it has been widely used in many fields. [0003] Collagen peptide is a new type of collagen product, which is produced from collagen or collagen-rich substances. It not only has the unique amino acid composition of collagen, but also has the characteristics of small molecular weight. Therefore, it is easier to be absorbe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78A61K8/65A61Q19/02A61Q19/08A61Q19/00
Inventor 熊康康廖龙兴熊赐权
Owner GUANGZHOU TUOLONG BIOTECH DEV
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