Antioxidant extract of primula sikkimensis as Tibetan drug as well as preparation method and application of antioxidant extract
A technology of Primula Sikkim and anti-oxidation effect, which is applied in the field of Tibetan medicine Primula Sikkim Antioxidant Extract and its preparation, can solve the problems of unreported chemical composition and anti-oxidation effect of Primula Sikkim. Reporting and other issues
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Embodiment 1
[0017] The preparation method of each extract:
[0018] 1) Total extract extraction
[0019] Take 20 g of whole herb powder of Primula Sikkim, soak it in 600 ml of 90% ethanol for 24 h, extract it with ultrasonic solution for 1 h, filter, repeat the above extraction process twice for the filter residue, filter, and combine the three extracts.
[0020] 2) concentrated
[0021] Ethanol was recovered from the extract under reduced pressure to obtain a concentrated solution (relative density 1.15g / mL, 55°C), and then the concentrated solution was mixed with water at a volume ratio of 1:1 to obtain a diluted concentrated solution (90 ml).
[0022] 3) Gradient extraction
[0023] Sequentially use petroleum ether (90 ml / time x 3 times), chloroform (90 ml / time x 3 times), ethyl acetate (90 ml / time x 3 times), n-butanol (90 ml / time x 3 times) Extract the diluted concentrate, combine the same organic phases, and concentrate under reduced pressure to obtain petroleum ether extract (XJ...
Embodiment 2
[0025] The preparation method of each extract:
[0026] 1) Total extract extraction
[0027] Take 30 g of whole herb powder of Primula Sikkim, soak it in 900 ml of 65% ethanol for 24 h, extract with ultrasonic solution for 1 h, filter, repeat the above extraction process twice for the filter residue, filter, and combine the three extracts.
[0028] 2) concentrated
[0029] Recover ethanol from the extract under reduced pressure to obtain a concentrated solution (relative density 1.10g / ml, 55°C), and then mix the concentrated solution with water at a volume ratio of 1:1 to obtain a diluted concentrated solution (100 ml).
[0030] 3) Gradient extraction
[0031] Sequentially use petroleum ether (100 ml / time x 3 times), chloroform (100 ml / time x 3 times), ethyl acetate (100 ml / time x 3 times), n-butanol (100 ml / time x 3 times) Extract the diluted concentrated solution, combine the same organic phases, and concentrate under reduced pressure to obtain petroleum ether extract (XJ...
Embodiment 3
[0033] Scavenging DPPH free radical activity experiment:
[0034] Activity experiments were carried out using the polar sites prepared in Example 1.
[0035] The antioxidant activity is expressed by the ability to scavenge DPPH free radicals. According to the experimental conditions determined in the pre-experiment, the activity of scavenging lipid free radical DPPH of different samples of Primula Sikkim was determined. Take 2.0 mL of sample solutions with different concentrations, add 2.0 mL of 1.52×10 -4 Mmol / L DPPH absolute ethanol solution was mixed well, and after standing in the dark at room temperature for 30 minutes, the solvent was used as a blank control, and the absorbance A was measured at 517 nm. 1 ; Replace the sample solution with the same volume of solvent as a negative control, and measure the absorbance A 2 ; Use the same volume of absolute ethanol instead of DPPH solution as a blank group to measure the absorbance A 3 . With VC as the control, each gro...
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