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Selection method of seed ginseng in cross-breeding of Apostichopus japonicus

A technology of hybrid breeding and seed ginseng, which is applied in the field of selection of seed ginseng in sea cucumber hybrid breeding, can solve the problems of seed ginseng waste, consumption of manpower and material resources, and inability to judge male and female, so as to reduce the waste of seed ginseng and avoid polyspermy , Improve the effect of fertilization rate

Active Publication Date: 2015-10-21
山东安源种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this move is that it is impossible to judge male and female in advance, resulting in the waste of a large number of ginseng species and consuming a lot of manpower and material resources.
Moreover, due to the heavy workload and many people, mistakes are often made during the busy schedule, and the asynchronousness of the sperm and eggs of the species of ginseng often leads to the failure of the experiment to proceed smoothly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Harvesting and screening

[0032] (1) Harvesting: In November of the Gregorian calendar every year, when the seawater temperature in the natural sea area reaches 10-12°C, harvesting is carried out in the excellent habitat environment of wild sea cucumbers. The water depth in the sea area is 5-10m, and the bottom quality It is sandy mud and gravel bottom, rich in seaweed resources such as eelweed, sargassum, and sargassum;

[0033] When diving for fishing, divers need to pay attention to the following:

[0034] ① The number of harvested at one time should not be too much, and the stimulation should be reduced as much as possible to avoid mechanical damage caused by the backlog of sea cucumbers;

[0035] ②The hands of divers and operators, or vessels, tools, etc. should not be in contact with oil stains;

[0036] ③Keep the water quality in the holding tank on board fresh and change the water in time;

[0037] ④ Harvested sea cucumbers should be protected from strong...

Embodiment 2

[0059] The stocking density is 20 heads / m 3 , the dissolved oxygen concentration in the storage tank is 8mg / ml;

[0060] The temperature raising method of the maturation-promoting cultivation process is as follows: after the ginseng is put into the cultivation tank, the water temperature is controlled at 3°C, kept for 3 days, and then raised to 6°C at a rate of 1°C / d, and then raised at a rate of 0.5°C / d after constant temperature cultivation for 8 days. Cultivate at a constant temperature for 8 days at a temperature of 10°C, and continue to rise to 15°C at a rate of 0.5°C / d. During the process of promoting ripening, the water is changed once a day, and the amount of water changed each time is 50% of the pool water. The water changed is high-quality seawater during the high tide of seawater, and is treated with filter sand. During the ripening process, the mixture of powdered feed and sea mud was fed, and the amount of feed fed accounted for 5% of the weight of the species in...

Embodiment 3

[0065] The stocking density is 18 heads / m 3 , the dissolved oxygen concentration in the storage tank is 7mg / ml;

[0066] The temperature raising method of the maturation-promoting cultivation process is as follows: after the ginseng is put into the cultivation pond, the water temperature is controlled at 2°C and kept for 3 days, then the temperature starts to rise at a rate of 1°C / d, and rises to 6°C. The rate was raised to 10°C, incubated at a constant temperature for 7 days, and continued to rise to 15°C at a rate of 0.5°C / d to wait for production. During the process of promoting ripening, the water is changed twice a day, and the amount of water changed each time is 40% of the pool water. The water changed is high-quality seawater during the high tide of seawater, and is treated with filter sand. During the process of promoting ripening, the mixture of powdered feed and sea mud was fed, and the feed amount accounted for 4% of the weight of ginseng in the pond, and the feed...

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Abstract

The invention discloses a selection method of breeding stichopus japonicus in stichopus japonicus cross breeding. The method comprises the following steps that (1) good breeding stichopus japonicus is collected and screened; (2) the screened breeding stichopus japonicus is placed into a holding pool to be subjected to the maturation acceleration culture through controlling the breeding stichopus japonicus density, the water temperature and the water quality; (3) the breeding stichopus japonicus beginning to enter the breeding period is subjected to miscarriage experiments, the breeding stichopus japonicus is stimulated for spermiation and ovulation, the sex discrimination is carried out, meanwhile, the mass discharge is inhibited, and in addition, male and female individuals are placed into different culture pools to be respectively cultured; (4) the breeding stichopus japonicus is cultured again for two days, the male and female individuals are quantificationally selected according to the requirement on the third day, and are placed into containers for mass discharge, and independent seminal fluid and ova can be obtained; (5) cross breeding or family culture is carried out. The method has the advantages that the fertilization rate of the ova is improved, and the breeding stichopus japonicus waste in the large-scale breeding production is reduced, the fertilization rate and the cross breeding rate are improved, the combination of the sperms and the ova of the stichopus japonicus of the same strain is avoided, and the technical support is provided for the cross breeding of the stichopus japonicus.

Description

technical field [0001] The invention relates to a method for selecting ginseng in cross-breeding of japonicus japonicus. Background technique [0002] Because of its high nutritional value, sea cucumber ranks first in the "Eight Treasures of Seafood". With the breakthrough of seedling breeding technology, the sea cucumber breeding industry has developed rapidly, and it has now developed into the species with the highest output value of a single species of mariculture in Shandong Province. Due to the demand for its different traits, major scientific research institutions and production units have used biotechnology to cultivate sea cucumbers with excellent traits such as "thorny" and "high temperature resistance". However, some problems were encountered during the breeding process. question. [0003] Because the appearance of sea cucumbers is roughly the same, it is difficult to distinguish between male and female with the naked eye, and a single male and female individual i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王亮邹士方王增东梁文恒刘永旗王静代文汇
Owner 山东安源种业科技有限公司
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