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Preparation method for adsorbent capable of removing heavy metals

A technology for adsorbents and heavy metals, applied in the field of preparation of adsorbents for removing heavy metals

Inactive Publication Date: 2013-12-25
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the research on the synthesis and adsorption of biological manganese oxides in the world is limited to the source of manganese nodules from the ocean or land. We synthesized manganese oxides under different pH conditions through the manganese oxidizing bacteria Pseudomonas sp.G7 derived from the surface soil.

Method used

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  • Preparation method for adsorbent capable of removing heavy metals
  • Preparation method for adsorbent capable of removing heavy metals
  • Preparation method for adsorbent capable of removing heavy metals

Examples

Experimental program
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specific Embodiment approach 1

[0013] Embodiment 1: Preparation of manganese oxides under different pH conditions

[0014] The 1L medium for the experiment consists of: 0.5g of yeast extract, 0.5g of hydrolase protein, CaCl 2 0.222g, MgSO 4 0.796g, glucose 0.5g, FeCl 3 1ml (3.7mM), trace elements 1ml (CuSO 4 ·5H 2 O 10mM, ZnSO 4 ·7H 2 O 44mM, CoCl 2 ·6H 2 O 20mM, NaMoO 4 2H 2 O 13mM). After the preparation of the medium, adjust the pH to 6-8 with 4M NaOH, sterilize with damp heat at 115°C for 30 minutes, inoculate 2% (V / V) manganese-oxidizing bacteria Pseudomonas sp. The buffer solution of bacteria, the final concentration is 20mM (the buffer solution is 4-hydroxyethylpiperazineethanesulfonic acid (HEPES) or 2-(N-morpholine) ethanesulfonic acid (MES), wherein HEPES adjusts the pH to 6.0- 7.5, MES to adjust the pH to 8.0), 27 ° C constant temperature shaking culture for 19 hours before adding filter-sterilized MnCl 2 (The final concentration is 1mM), and continuously cultivated at 27°C for 2 w...

specific Embodiment approach 2

[0016] Specific embodiment two: Scanning electron microscope observation (SEM) and energy spectrum analysis (EDX) of manganese oxide

[0017] Table 1 Energy spectrum analysis results

[0018]

[0019] Note: K and L refer to the excitation energy of the K and L layers respectively.

[0020] The manganese oxide synthesized under the condition of pH 7.5 was observed by scanning electron microscope and analyzed by energy spectrum (S-3000N, HITACIII Co., Japan). The results of scanning electron microscopy and energy dispersive spectroscopy were as follows: figure 1 As shown in Table 1, aggregates of manganese oxide can be formed outside the microorganism, and a higher content of manganese oxide can be obtained through biological methods, with a mass percentage of 22.40%.

specific Embodiment approach 3

[0021] Specific embodiment three: the adsorption of manganese oxide to heavy metal Cu, Pb

[0022] Take 5ml manganese oxide suspension, add 2.5ml 0.1M NaO 3and different amounts of heavy metals, the pH was adjusted to 5.0+0.1, and the total volume was 25ml. Shake at 25°C for 24 hours, centrifuge at 13000g, and measure the concentration of heavy metal ions in the supernatant by inductively coupled plasma emission spectrometry (ICP-OES, OPTMA2000, Perk Elmer Co, U.S.A.), the difference between the concentration of heavy metals in the supernatant and the initial concentration All adsorption experiments were repeated 3 times for the adsorption amount of heavy metals on manganese oxides. It is known from the specific embodiment one that the concentration difference of manganese oxide in the suspension prepared under the conditions of pH 6.0 and 8.0 is the largest, which is 14.35%, but from figure 2 a and figure 2 It is known from b that after the adsorption reaches equilibrium...

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Abstract

A preparation method for an adsorbent capable of removing heavy metals such as Cu and Pb comprises: dropwise adding a 4M NaOH solution into a selective medium solution to adjust pH of the medium to 6-8; inoculating 2% (V / V) manganese oxidating bacteria pseudomonas sp.G7 strain to the medium solution which is subjected to heat sterilization with steam for 30 min at 115 DEG C, then adding a filtered disinfected buffer solution to again adjusting pH of the solution to 6-8; performing concussion culture for 12-24 h at 20-30 DEG C, adding filtered disinfected MnCl2 (to make the final concentration at 1 mM); continuously culturing for 1-3 weeks at 20-30 DEG C to aggregate a large amount of dark brown even black manganese oxides on the surface of bacterium colonies; and centrifuging with centrifugal force of 7500 g for 10-20 min to remove the medium, washing with a phosphatic buffer solution for 3-5 times, and finally dissolving surface adsorbed Mn<2+> with a 20-80 mM CuSO4 buffer solution to obtain biological manganese oxides.

Description

technical field [0001] The present invention relates to a kind of preparation method that removes heavy metal adsorbent, specifically, it relates to a kind of utilization manganese oxidizing bacteria Pseudomonas sp.G7 to prepare amorphous manganese oxide and this kind of manganese oxide to heavy metal Cu, Pb adsorption aspects of application. technical background [0002] The content of manganese in the earth's crust is 0.085%, and it is the most common heavy metal element after iron in abundance. Manganese oxide minerals widely exist in continents and ocean bottoms. The reserves on the ocean bottom are tens to thousands of times the corresponding reserves on the land. It is difficult to mine. The distribution of manganese resources on land is uneven, and most of them are small in scale. Manganese minerals mainly include pyrolusite (MnO 2 ), manganese ore (mMnO·MnO 2 ·nH 2 O), manganese (Mn 2 o 3 ·H 2 O), brown manganese (Mn 2 o 3 ), black manganese ore (Mn 3 o 4 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/06B01J20/30C12P3/00C12R1/38
Inventor 胡春杨振东
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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