Method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide

An astragalus polysaccharide and countercurrent ultrasonic technology are applied in the field of phytochemistry, can solve the problems of polysaccharide structure damage, complicated processing procedures, etc., and achieve the effects of high extraction rate, simple operation, and accelerated dissolution rate.

Inactive Publication Date: 2013-12-25
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkaline extraction can also cause damage to the polysaccharide structure
In addition, neutralization is required after acid and alkali leaching, and the subsequent treatment procedures are complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Crush Astragalus membranaceus, immerse it in distilled water according to the mass volume ratio of 1:5, then conduct continuous countercurrent ultrasonic extraction at 50°C and 40KHz for 1 hour, centrifuge, take the supernatant, decolorize with activated carbon, and obtain the aqueous extract solution of astragalus polysaccharide; Concentrate to the astragalus polysaccharide concentrate with a solid content of 30%, add 2 times the amount of 80% ethanol solution and stir for 25 minutes; centrifuge, and dilute the precipitate with 5 times distilled water; the diluted solution is filtered with an ultrafiltration membrane, and the ultrafiltration membrane The molecular weight cut-off was 5000 Daltons, and the filtrate was precipitated with alcohol to obtain high-purity astragalus polysaccharide solids with a purity of 88.6%.

Embodiment 2

[0018] Crush Astragalus membranaceus, immerse it in distilled water according to the mass volume ratio of 1:8, then conduct continuous countercurrent ultrasonic extraction at 65°C and 80KHz for 1.5h, centrifuge, take the supernatant, decolorize with activated carbon, and obtain the aqueous extract solution of astragalus polysaccharides ;Concentrate to the astragalus polysaccharide concentrate with a solid content of 35%, add 3 times the amount of 85% ethanol solution and stir for 30min; centrifuge, and dilute the precipitate with 4 times distilled water; The molecular weight cut off is 8000 Daltons, and the filtrate is alcohol-precipitated to obtain high-purity astragalus polysaccharide solids with a purity of 94.3%.

Embodiment 3

[0020] : Grind Astragalus membranaceus, immerse in distilled water according to the mass volume ratio of 1:10, then conduct continuous countercurrent ultrasonic extraction at 70°C and 60KHz for 2 hours, centrifuge, take the supernatant, decolorize with activated carbon, and obtain the aqueous extract solution of Astragalus polysaccharides ;Concentrate until the astragalus polysaccharide concentrate with a solid content of 40%, add 4 times the amount of 90% ethanol solution and stir for 30min; centrifuge, and dilute the precipitate with 3 times distilled water; The molecular weight cut off is 3000 Daltons, and the filtrate is alcohol-precipitated to obtain high-purity astragalus polysaccharide solids with a purity of 91.8%.

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Abstract

The invention discloses a method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide. The method comprises the following processing steps: crushing astragalus membranaceus and soaking into distilled water; centrifuging and taking supernate after continuous counter-current ultrasonic extraction; decoloring by active carbon, so as to obtain an astragalus polysaccharide water extract solution; then concentrating and adding ethanol to stir; centrifuging and diluting sediments by the distilled water; carrying out alcohol precipitation after filtering diluents by a membrane, wherein the sediment is astragalus polysaccharide solid. By adopting the method, the dissolution rate of the astragalus polysaccharide can be improved; the extraction time is shortened; the dosage of a solvent is reduced. Therefore, the method has the characteristics of high selectivity, simple process, low energy consumption, high yield, high product purity and the like.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a method for continuous countercurrent ultrasonic extraction of high-purity astragalus polysaccharides. Background technique [0002] Astragalus is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. Sweet in taste and slightly warm in nature, it has the effects of invigorating qi and solidifying the surface, diuresis and detoxification, evacuating pus, astringing sores and promoting granulation. Astragalus membranaceus has relatively high medicinal value. Modern pharmacology and traditional Chinese medicine chemistry studies have shown that Astragalus membranaceus contains astragalus polysaccharides, astragaloside IV and various amino acids, among which astragalus polysaccharides are the most abundant one, and its main biological activity is to enhance immune function. , anti-influenza, anti-tumor, anti-aging and anti-radia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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