Natural pigment prepared by using fruit residues and applications thereof
A technology of natural pigments and pomace, applied in the field of natural pigments
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Embodiment 1
[0016]Preparation of purified mulberry pomace extract: take Dashi variety mulberries and squeeze the juice, and then separate the juice and pomace by centrifugation or wrapping the pomace with filter cloth and extruding. The fruit pomace was soaked and extracted with 50% ethanol solution with a volume ratio of 1:1 and pH 4.0 for 3 times, each time for 12 hours, then filtered with suction, combined with the extracts, centrifuged for 20 minutes (5000 rpm), and the supernatant was taken, and washed with flashlight. The steaming and concentrating device vacuum-concentrates at 50°C until there is no alcohol smell, and the pomace concentrate is obtained. The fruit pomace concentrate was passed through a macroporous resin Diaion HP 2MGL column, washed with distilled water until it was colorless and transparent, and then eluted with 60% ethanol solution with pH 4.0 at a flow rate of 0.6 mL / min to collect colored cyanine The element eluate was concentrated under reduced pressure at 50 ...
Embodiment 2
[0020] Preparation of purified mulberry pomace extract: take Japanese mulberry varieties and extract juice, then centrifuge or use filter cloth to wrap pomace and squeeze to separate juice and pomace. The fruit pomace was soaked and extracted with 55% ethanol solution with a volume ratio of 1:1 and pH 4.0 for 4 times, each time for 16 h, then filtered with suction, combined with the extracts, centrifuged for 20 min (5000 rpm), and the supernatant was taken, and washed with flashlight. The steaming and concentrating device vacuum-concentrates at 50°C until there is no alcohol smell, and the pomace concentrate is obtained. The pomace concentrate was passed through a macroporous resin Diaion HP 2MGL column, washed with distilled water until it was colorless and transparent, and then eluted with 65% ethanol solution with pH 4.5 at a flow rate of 0.6 mL / min to collect colored cyanine The element eluate was concentrated under reduced pressure at 50 o C using a flash concentration de...
Embodiment 3
[0024] Preparation of purified mulberry pomace extract: extract juice from mulberries of red agate variety, and then separate juice and pomace by centrifugation or wrapping pomace with filter cloth and extruding. The fruit pomace was soaked and extracted three times in 55% ethanol solution with a volume ratio of 1:1.5 and pH 4.0, each time for 15 h, then filtered with suction, combined the extracts, centrifuged for 20 min (5000 rpm), and the supernatant was taken, and washed with flashlight. The steaming and concentrating device vacuum-concentrates at 50°C until there is no alcohol smell, and the pomace concentrate is obtained. The fruit pomace concentrate was passed through a macroporous resin Diaion HP 2MGL column, washed with distilled water until it was colorless and transparent, and then eluted with 60% ethanol solution at pH 4.0 at a flow rate of 0.5 mL / min to collect colored cyanine The element eluate was concentrated under reduced pressure at 50 o C using a flash conce...
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