Tea flower oligosaccharide or polysaccharide, preparation method thereof and application thereof in cigarettes
A technology of tea tree flowers and oligosaccharides, which is applied in the fields of spices and tobacco, and can solve the problems of rarely used tea tree flowers and self-destruction
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Embodiment 1
[0068] Embodiment 1: 1# tea tree flower oligosaccharide, 2# tea tree flower polysaccharide, and 1# cigarette and 2# Preparation of cigarettes
[0069] 1. Experimental materials
[0070] Tea tree flowers are dried flowers of purchased Longjing tea tree flowers.
[0071] 2. Preparation of tea tree flower oligosaccharides and polysaccharides
[0072] Proceed as follows:
[0073] (1) Water extraction: Weigh a certain amount of dried Longjing tea tree flowers, and use hot water extraction for 3 extractions, the mass ratio of solvent to tea tree flowers is 5;
[0074] (2) Liquid-solid separation: use a 200-mesh filter cloth to separate the extract from the tea tree flower to obtain the extract, and combine the extracts obtained by 3 extractions;
[0075] (3) Microfiltration: use a microfiltration membrane with a pore size of 50nm, and the system pressure is 6Bar;
[0076] (4) Ultrafiltration: Use an ultrafiltration membrane with a molecular weight cut-off of 5000Dal for sepa...
Embodiment 2
[0086] Embodiment 2: the preparation of 3# tea tree flower oligosaccharide, 4# tea tree flower polysaccharide and 3# cigarette
[0087] 1. Experimental materials
[0088] The tea tree flowers are collected from the Tieguanyin tea tree in Anxi, Fujian.
[0089] 2. Preparation of tea tree flower oligosaccharides and polysaccharides
[0090] Proceed as follows:
[0091] (1) Water extraction: Weigh a certain amount of Tieguanyin, and use microwave extraction method to extract twice, and the mass ratio of solvent to tea tree flower is 15;
[0092] (2) Liquid-solid separation: the extract is separated from the tea tree flower by centrifugation at a centrifugation rate of 9000 rpm for 5 minutes, and the extract obtained by 2 extractions is combined;
[0093] (3) Microfiltration: use a microfiltration membrane with a pore size of 800nm, and the system pressure is 1Bar;
[0094] (4) Ultrafiltration: Use an ultrafiltration membrane with a molecular weight cut-off of 300,000Dal for...
Embodiment 3
[0100] Embodiment 3: 5# tea tree flower oligosaccharide, 6# tea tree flower polysaccharide, and 4#-6# cigarette preparation
[0101] 1. Experimental materials
[0102] Tea tree flowers were purchased from Keemun black tea trees in Qimen County, Anhui.
[0103] 2. Preparation of tea tree flower oligosaccharides and polysaccharides
[0104] Proceed as follows:
[0105] (1) Water extraction: Weigh a certain amount of dry tea tree flowers of Keemun black tea, and use the ultrasonic extraction method to extract 3 times, the mass ratio of solvent to tea tree flowers is 25;
[0106] (2) Liquid-solid separation: the extraction solution is separated from the tea tree flower by suction filtration to obtain the extraction solution, and the extraction solution obtained by 3 extractions is combined;
[0107] (3) Microfiltration: use a microfiltration membrane with a pore size of 200nm, and the system pressure is 3Bar;
[0108] (4) Ultrafiltration: Use an ultrafiltration membrane wi...
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