Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing jellyfish gonad hydrolyzed protein

A technology for hydrolyzing protein and gonads, which is applied in the field of preparing jellyfish gonad hydrolyzed proteins, which can solve the problems of no jellyfish gonad hydrolyzed proteins, etc., and achieve the effects of fast preparation speed, simple and convenient operation, and low production cost

Inactive Publication Date: 2014-01-22
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common hydrolyzed proteins include fish hydrolyzed protein, shellfish hydrolyzed protein and collagen hydrolyzed protein, but no jellyfish gonad hydrolyzed protein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing jellyfish gonad hydrolyzed protein

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0015] The preparation process steps of jellyfish gonad hydrolyzed protein are:

[0016] 1) Thaw the jellyfish gonads frozen at -20°C at -4°C, wash with water to remove impurities, and grind them with a high-speed tissue homogenizer.

[0017] 2) Take a certain amount of minced jellyfish gonads and put them in a three-neck flask, place them in a water bath, stir them, and adjust the pH to 4-7.5 after reaching the predetermined temperature of 30-60°C. The ratio of enzyme to substrate jellyfish gonads is Add flavor protease at 600-2000U / g, and the solid-liquid ratio of jellyfish gonad and water is 1:8-20, and react for 2-24h.

[0018] 3) After enzymatic hydrolysis, heat the mixed slurry at 95-100°C for 10 minutes to inactivate flavor protease. After inactivation, the mixture was centrifuged at 4°C (4000-6000g, 8-15min), the supernatant was taken, and the degree of protein hydrolysis was determined by formaldehyde titration.

[0019] 4) Spray-dry the enzymatic hydrolysis concent...

Embodiment 1

[0021] 1) Thaw the jellyfish gonads frozen at -20°C at -4°C, wash with water to remove impurities, and grind them with a high-speed tissue homogenizer.

[0022] 2) Put the minced jellyfish gonads into a three-necked flask and place them in a water bath for stirring. After reaching the predetermined temperature of 30°C, adjust the pH to 4, and add flavor protease according to the ratio of enzyme to substrate jellyfish gonads at 1600U / g , The solid-liquid ratio of jellyfish gonads (g) to water (ml) is 1:8, react for 4 hours for enzymatic hydrolysis.

[0023] 3) After enzymatic hydrolysis, heat the mixed slurry at 95-100°C for 10 minutes to passivate the flavor protease and inactivate it. The mixture was centrifuged at 4°C (6000g, 10min), the supernatant was taken, and the degree of protein hydrolysis was determined to be 24.65% by formaldehyde titration.

[0024] 4) Concentrate the enzymatic solution of the jellyfish gonad after enzymolysis in step 2 to a concentration of 8-20%...

Embodiment 2

[0026] 1) Thaw the jellyfish gonads frozen at -20°C at -4°C, wash with water to remove impurities, and grind them with a high-speed tissue homogenizer.

[0027] 2) Put the minced jellyfish gonads into a three-necked flask and place them in a water bath for stirring. After reaching the predetermined temperature of 50°C, adjust the pH to 5.5, and add flavor protease according to the enzyme-to-bottom ratio of 1200U / g, and the solid-to-liquid ratio is 1 : 10, reaction 6h.

[0028] 3) After enzymatic hydrolysis, heat the mixed slurry at 95-100°C for 10 minutes to passivate flavor protease and inactivate it. The mixture was centrifuged at 4°C (5000g, 15min), the supernatant was taken, and the degree of protein hydrolysis was determined to be 38.23% by formaldehyde titration.

[0029] 4) Concentrate the enzymatic solution of the jellyfish gonads after enzymolysis in step 2 to a concentration of 8-20% of the hydrolyzed protein, and spray-dry the concentrated solution to obtain light ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of marine biotechnology and particularly relates to a method for preparing a jellyfish gonad hydrolyzed protein. According to the method, jellyfish gonads are subjected to enzymolysis and then spray drying processing is carried out to finally obtain the jellyfish gonad hydrolyzed protein. According to the method, easily assimilated micromolecular hydrolyzed protein is obtained by using sufficient jellyfish gonad resources and can be used in the fields of foods and health-care products and the like; therefore, the invention opens up a new way for improving the utilization degree of jellyfish resources.

Description

technical field [0001] The invention relates to the technical field of marine organisms, in particular to a method for preparing jellyfish gonad hydrolyzed protein. Background technique [0002] Jellyfish is a colloidal zooplankton distributed in China, Japan, the coast of the Korean Peninsula and the Russian Far East. There are jellyfish distributed in the vast sea area from the Yalu River Estuary in the north to the Beibu Gulf in the south, and the resources are rich. my country is the first country to use jellyfish resources, but at this stage it is only pickled and used as food. The gonads of jellyfish are located in the mouth and wrist, commonly known as jellyfish brain and jellyfish flower. Jellyfish gonads are delicious and rich in amino acids. However, during the processing of jellyfish, the gonads are usually thrown away, and only a small part is boiled in boiling water and then added with condiments for consumption, resulting in waste of resources and environment...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23J1/04
Inventor 于华华李鹏程李荣锋邢荣娥刘松秦玉坤陈晓琳胡林峰
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com