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Preparation method of compound microbial inoculum for degrading domestic sewage

A technology for domestic sewage and compound bacterial agent, which is applied in microorganism-based methods, biochemical equipment and methods, biological water/sewage treatment, etc., can solve problems such as low nitrogen and phosphorus removal rate, and achieve low environmental pollution and low production costs. , the effect of good conversion efficiency

Inactive Publication Date: 2014-01-29
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to solve the problem of low removal rate of nitrogen and phosphorus in constructed wetland sewage in the existing low temperature environment, and to provide a preparation method for degrading domestic sewage composite bacterial agent

Method used

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  • Preparation method of compound microbial inoculum for degrading domestic sewage
  • Preparation method of compound microbial inoculum for degrading domestic sewage
  • Preparation method of compound microbial inoculum for degrading domestic sewage

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specific Embodiment approach 1

[0051] Specific embodiment one: the preparation method of this embodiment degrades domestic sewage compound bacterial agent, realizes according to the following steps:

[0052] 1. Pseudomonas, Bacillus, Nitrobacter, Nitrosomonas and Bacillus megaterium were respectively inoculated in the sterilized domestic sewage medium for cultivation at a temperature of 15°C and a shaker speed of 130r / min Under certain conditions, the number of bacteria in the bacterial solution reached 3.6×10 8 ~9.3×10 8 pcs / ml, 2.5×10 8 ~7.8×10 8 pcs / ml, 1.5×10 8 ~6.6×10 8 pcs / ml, 5.0×10 8 ~6.8×10 8 / ml and 2.5×10 8 ~8.6×10 8 a / ml;

[0053] 2. Mix the bacteria liquid of Pseudomonas and Bacillus to obtain COD degrading compound bacteria, and mix the bacteria liquid of Nitrobacter and Nitrosomonas to obtain nitrifying and nitrosifying compound bacteria and giant spores The bacteria liquid of bacillus is the phosphorus removal bacteria;

[0054] 3. Mix COD-degrading composite bacteria, nitrifying ...

specific Embodiment approach 2

[0057] Embodiment 2: This embodiment is different from Embodiment 1 in that the sterilization condition of the sewage in the domestic sewage culture medium in Step 1 is 121° C. for 30 minutes. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0058] Specific embodiment three: what this embodiment is different from specific embodiment one or two is that in step 3, COD degrades composite bacteria, nitrification and nitrosification composite bacteria and phosphorus removal bacteria are under the condition of the same number of bacteria, according to volume ratio 10: 0.25:0.5 for mixing. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a preparation method of a compound microbial inoculum for degrading domestic sewage, relating to a preparation method of compound microbial inocula and solving the problem in the prior art of low nitrogen and phosphorus removal rate of sewage in artificial wetland at a low temperature environment. The preparation method comprises the following steps of: I, respectively inoculating pseudomonas, bacillus, nitrobacter, nitrosomonas and bacillus megatherium in a sterilized domestic sewage culture medium for culturing to obtain a respective inoculum; II, preparing COD (Chemical Oxygen Demand) degradable compound bacteria, preparing nitrobacter and nitrosomonas compound inocula, and taking the bacillus megatherium inoculum as phosphorous removal bacteria; and III, mixing the COD degradable compound bacteria, the nitrobacter and nitrosomonas compound inocula and the phosphorous removal bacteria under the condition of the same bacterial count. The prepared compound microbial inoculum for degrading domestic sewage is strong in adaptability to the environment, suitable for microorganism enhanced sewage treatment in the northern area, the ammonia nitrogen conversion rate of sewage can reach 95%, the total nitrogen conversion rate reaches 82%, the COD conversion rate reaches 60%, and the total phosphorus conversion rate is about 75%.

Description

technical field [0001] The invention relates to a preparation method of a composite microbial agent. Background technique [0002] With the increasingly serious problem of water pollution, the effective control of water pollution is urgent. The main substances polluting water bodies are organic matter, total nitrogen and total phosphorus. Therefore, the removal of carbon, nitrogen, and phosphorus is the key to water pollution control. Among all sewage treatment technologies, biochemical methods are the most effective and common. However, conventional biochemical methods mainly remove organic matter in sewage, and there are certain restrictions on the removal effect of nitrogen and phosphorus. If the advanced treatment of nitrogen and phosphorus is supplemented in the later stage of the conventional biochemical method, it will inevitably increase the cost and energy consumption of sewage treatment. Therefore, how to efficiently remove carbon, nitrogen, and phosphorus in s...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/07C12R1/11C12R1/01
Inventor 杨基先赵昕悦邱珊魏清娟焉志远朱世殊徐善文马放
Owner HARBIN INST OF TECH
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