Oilfield on-site production method of oil-displacement microbial inoculum

A technology for oil-displacing microorganisms and a production method, which is applied in the field of microbial oil-displacing bacterial agent production, and can solve the problems of affecting the oil-displacing effect of microbial composition and reducing the proportion of the oil.

Inactive Publication Date: 2014-02-05
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The nutrients needed by oil-displacing microorganisms include fish meal, yeast extract, peptone, molasses, corn steep liquor, inorganic salts, etc. These nutrients contain a certain amount of microorganisms, so without sterilization treatment, oil-displacing microorganisms will be fermented on site When , the proportion gradually decreases, which affects the composition of microorganisms in the injected bacterial agent and the oil displacement effect after injection.

Method used

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  • Oilfield on-site production method of oil-displacement microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1). The Pseudomonas aeruginosa bacterial strain is produced in the factory to obtain 300L of seed solution, which is transported to the oilfield site.

[0018] 2). The fermentation medium of Pseudomonas aeruginosa is: molasses 7g / L, yeast extract 2g / L, fish meal 3g / L, NaNO 3 10g / L, K 2 HPO 4 0.4g / L, MgSO 4 ·6H 2 O0.2g / L. In the batching tank, molasses 42kg, yeast paste 12kg, fish meal 18kg, NaNO 3 60kg,K 2 HPO 4 2.4kg, MgSO 4 ·6H 2 O1.2kg, add 1.2 tons of drinking-grade tap water or underground water, and heat to 100°C for 10min, then transfer to the fermenter by pump.

[0019] 3). Add drinking-grade tap water or underground water into the fermenter and dilute to a total volume of 5.7 tons of the culture medium, and at the same time adjust the pH to 7.0 with the acid-base solution.

[0020] 4). Inoculate the high-activity seed liquid produced by the factory, the inoculum amount of the seed liquid is 5% (V / V) of the total volume of the fermentation liquid, that...

Embodiment 2

[0023] 1). The Bacillus subtilis strain is produced in the factory to obtain 1.2 tons of seed liquid, which is transported to the oilfield site.

[0024] 2). The fermentation medium of Bacillus subtilis is: sucrose 5g / L, peptone 4g / L, corn steep liquor 1g / L, NH 4 Cl7g / L, KH 2 PO 4 1g / L, MgSO 4 ·6H 2 O0.3g / L, CaCl 2 0.1g / L. In the batching tank, 30kg of sucrose, 24kg of peptone, 6kg of corn steep liquor, NH 4 Cl42kg, KH 2 PO 4 6kg, MgSO 4 ·6H 2 O1.8kg, CaCl 2 0.6kg, add 300L of drinking-grade tap water or underground water, and heat to 85°C for 30min, then transfer to the fermenter by pump.

[0025] 3). Add drinking-grade tap water or underground water into the fermenter to dilute the total volume of the culture medium to 4.8 tons, and at the same time adjust the pH to 7.4 with the acid and alkali solution.

[0026] 4). Inoculate the high-energy seed liquid produced by the factory, the inoculum amount of the seed liquid is 20% (V / V) of the total volume of the fermen...

Embodiment 3

[0029] 1). Each of Pseudomonas aeruginosa and Bacillus subtilis strains was produced in the factory to obtain 0.3 tons of seed liquid, and transported to the oilfield site.

[0030] 2). The suitable fermentation medium is molasses 7g / L, yeast extract 2g / L, fish meal 3g / L, NaNO 3 10g / L, K 2 HPO 4 0.4g / L, MgSO 4 ·6H 2 O0.2g / L. In the batching tank, molasses 42kg, yeast paste 12kg, fish meal 18kg, NaNO 3 60kg,K 2 HPO 4 2.4kg, MgSO 4 ·6H 2 O1.2kg, add 0.8 tons of drinking-grade tap water or underground water, and heat to 90°C for 20min, then transfer to the fermenter by pump.

[0031]3). Add drinking-grade tap water or underground water into the fermenter and dilute to a total volume of 5.4 tons of the culture medium, and at the same time adjust the pH to 7.4 with the acid-base solution.

[0032] 4). Inoculate the high-energy seed liquid produced by the factory, the inoculum amount of the seed liquid is 10% (V / V) of the total volume of the fermentation liquid, that is, a...

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Abstract

The invention relates to an oilfield on-site production method of a fermentative microbial solution from oil-displacement microbes, belonging to the field of production of a microbe oil-displacement microbial inoculum. The oil-displacement microbes are oil-displacement microbe strains which have been screened and determined, comprise endogenous and exogenous microbes, and can enhance the oil recovery ratio. The method comprises the following steps: boiling 5-20 times of culture medium in a proportioning tank, transferring into a fermentation tank, directly adding untreated domestic drinking tap water or underground water to dilute to the required concentration, inoculating a 1-20% (V/V) seed solution, and carrying out constant-temperature fermentation according to the requirements of strain growth for an appropriate time period, wherein the fermentative microbial inoculum is used for oil well injection. The method does not need to perform high-pressure steam sterilization and asepsis on the culture medium, can implement simple low-cost on-site production of the oil-displacement microbial inoculum, can enhance the on-site production efficiency of the microbial inoculum, reduces the production and transportation cost of the microbial inoculum factory, and ensures the activity of the microbial inoculum. The method provides a fermentative microbial inoculum on-site production method which is simple to operate, reduces the problem of infectious microbe pollution, and enhances the oil displacement effect of the microbes.

Description

technical field [0001] The invention relates to a method for oil-displacing microorganisms to produce fermented bacterial agents on the oilfield site, which belongs to the field of microbial oil-displaced bacterial agents, and specifically relates to the large-scale fermented production at the oilfield site without the need for high-pressure steam, filtration and other sterilization treatments for microbial fermented nutrients . Background technique [0002] Enhanced oil recovery is one of the key issues in the oil extraction industry. In recent years, a lot of research and field experiments have been carried out at home and abroad on the use of oil-displacement microbial agents and metabolites in oilfields to drive oil, reduce viscosity, and improve emulsification. The analysis of the on-site implementation shows that not only the amount of bacterial agent injection is small, but also the scale of oil field application is small. This is because the fermentation production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582E21B43/22C12R1/385C12R1/125C12R1/265C12R1/06C12R1/01
Inventor 熊晓辉陆利霞刘洋郭汉卿王雪梅
Owner NANJING UNIV OF TECH
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