Gordoniasinensis, preparation method and application

A technology of Gordonia sinensis and seeds, applied in the field of biomedical engineering, can solve the problems of ineffective prevention and treatment of chemical drugs, generalized systemic reactions, and the occurrence of various diseases, etc., to achieve The effect of a wide range of raw material sources

Active Publication Date: 2014-02-05
湖南微草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] As we all know, the nervous system, endocrine system and immune system of the human body are closely connected together to form a systemic regulatory network, that is, the neural-endocrine-immune network (NEI network), which dominates various functions of the body. Abnormal function will immediately cause different de

Method used

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  • Gordoniasinensis, preparation method and application
  • Gordoniasinensis, preparation method and application
  • Gordoniasinensis, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kind of Gordonia sinensis sq-15, its screening process is:

[0039] Make a plate with Cha's medium and expose it to the air for 15 minutes by natural sedimentation method (Hubei, Wuhan), cover the plate and place it in a constant temperature incubator at 25-32°C for about 2 days, and then select a single colony, and after many times A strain of Gordonia sinensis sq-15 was obtained by suboptimal culture. The sequence of its 16Sr RNA is shown in SEQ ID NO.1. Its 16Sr RNA is 99% similar to the 16Sr RNA of many strains of Gordonia, and its physiological and biochemical characteristics are quite different from those of the known Gordonella.

[0040] The bacterium is cultivated in 1sp-2 medium at a temperature of 30°C±1°C, and the liquid culture is vibrated at 150-180rpm for 2-3 days. Keep the slant of the excellent strains at 4°C. (The 1sp-2 medium is listed in Appendix 1 of the 2012 Edition of the Chinese Agricultural Strain Catalog, and the medium number is 0038). Lo...

Embodiment 2

[0044] A preparation method of Gordonia sinensis sq-15, the process is as follows:

[0045] 1) Inoculate it on a slant (1sp-2 culture medium) at 30°C±1°C, and culture for 2-3 days without microscopic examination of bacteria growing evenly. Transfer to the inoculation bottle.

[0046] 2) Transfer the seeds in the inoculation bottle to the seed tank (medium raw material sucrose 10kg / T, peptone 2kg / T, K 2 HPO 4 1.3kg / T, MgSO 4 0.2kg / T, (NH 4 ) 2 HPO 4 1.5kg / T, water balance, PH7.0~7.2) 30℃±1℃, ventilation rate 1:0.6, automatically add defoamer. Up to 30 ~ 32h sampling microscopic examination, no bacteria and healthy cells, stop fermentation,.

[0047] 3) into the fermentation tank (medium sucrose 20kg / T, peptone 2kg / T, K 2 HPO 4 1.3kg / T, MgSO 4 0.2kg / T, (NH 4 ) 2 HPO 4 2kg / T, water balance, pH7.0~7.2) cultivation temperature 30℃±1℃, air flow 1:0.8 and automatic feeding of defoamer. Microscopic examination at about 48-52, the bacteria aged into short rods, and the nu...

Embodiment 3

[0051] The influence of the Gordonia sinensis sq-15 immunomodulator on the immune function of mice, the experimental steps are:

[0052] The experimental animals were 62 Kunming male mice with a body weight of 20±2g. They were randomly divided into four groups. Distilled water + feed was CK (20 rats), and 0.0001g, 0.001g, and 0.01g of Gordonia sinensis powder were added to the feed respectively. , 14 each, fed continuously for 14 days, carried out spleen lymphocyte transformation on the 15th day, induced the experiment of producing IL-2TNF and thymus weight, the method was carried out according to the conventional method, the results are shown in Table 1 and Table 2.

[0053] Table 1 Effect of Gordonia sinensis sq-15 on mouse spleen lymphocyte transformation

[0054] group

Number of mice N

normal saline

conA-induced

CK

20

3860±477

11241±1722

0.0001g dose

14

8373±422*

18277±3168*

0.001g dose

14

11264±2329...

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Abstract

The invention discloses gordoniasinensis, a preparation method and application. Gordonia sinensis sq-15 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2013065 can generate muramyl dipeptide, lipopolysaccharide, carotene, carotenoid, flavone and a plurality of amino acids, in particular gamma-aminobutyric acid, vitamin and a plurality of trace elements in a czapek's medium. The generated components are organically and harmoniously in a system, so that the physiological function of the system can be sufficiently enhanced; the gordoniasinensis can play a synergetic effect when being combined with other inoculums, such as bacillus subtilis, bacillus licheniformis, or selenium enriched saccharomyces cerevisiae, to prepare different medicinal compositions, and can effectively improve the body immunity to achieve the aims of resisting oxidation, regulating intestinal tracts, and regulating heart vessels.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a strain of Gordonia sinensis sq-15, a preparation method of Gordonia sinica sq-15, and an application of Gordonia sinica sq-15. Background technique [0002] As we all know, the nervous system, endocrine system and immune system of the human body are closely connected together to form a systemic regulatory network, that is, the neural-endocrine-immune network (NEI network), which dominates various functions of the body. Abnormal function will immediately cause different degrees or forms of systemic reactions, and various diseases will appear. In the treatment of many infectious diseases, the immune function of cells plays an important role, while some sub-health and diseased Individuals are also due to low immunity so that the effect of pure chemical drug prevention and treatment is not good. Therefore, the use of biological mediators (immunomodulators) in the treatment of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16A61K35/74A61P37/02A61P39/06A61P1/14A61P9/00C12R1/01
Inventor 张素琴康建南张世敏
Owner 湖南微草生物科技有限公司
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