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Quality control method and application of baccharis type propolis

A Dionysus chrysanthemum and propolis technology, which is applied in the field of Bacchus genus type propolis quality control, can solve problems such as difficulty in reflecting the biological activity of Brazilian Dionysus chrysanthemum type propolis, and achieves high sensitivity, good separation effect and strong feasibility. Effect

Active Publication Date: 2015-07-08
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, referring to China’s national propolis standards, it is difficult to reflect the biological activity of Brazilian Dionysus propolis, and even poplar propolis or even poplar gum is used as Brazilian Dionysus propolis.

Method used

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  • Quality control method and application of baccharis type propolis
  • Quality control method and application of baccharis type propolis
  • Quality control method and application of baccharis type propolis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C reference substance 10, 10, 10, 40, 10 mg respectively, and dilute to 10 mL with methanol. Then take 0.5, 0.2, 0.5, 0.25, 4 mL respectively and dilute to 10 mL with methanol as the standard mixture, and analyze by HPLC.

[0024] Take a certain amount of propolis samples of Dionysus genus to be tested, freeze, pulverize and mix, take 0.25g, and use 80% ethanol for ultrasonic extraction. Constant volume, after filtering through a microporous membrane, use high performance liquid chromatography to measure.

[0025] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL.

[0026] The mobile phase is: pure methanol and 1% acetic acid water mixture by volume ratio, and the binary gradient is eluted by volume ratio. The elution program is: 0-10 min, methanol 15-25%; 10-25 min,...

Embodiment 2

[0029] Weigh chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C reference substances 10, 10, 10, 40, 10 mg respectively, and dilute to 10 mL with methanol. Then take 0.5, 0.2, 0.5, 0.25, 4 mL respectively and dilute to 10 mL with methanol as the standard mixture, and analyze by HPLC.

[0030] A certain amount of poplar-type propolis and poplar gum samples were taken for freezing, pulverization and mixing, and 0.25 g of each was used for ultrasonic extraction with 70% ethanol. The ratio of solid to liquid was 1:45. 70% ethanol to constant volume, filtered through a microporous membrane, and then determined by high performance liquid chromatography.

[0031] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL.

[0032]The mobile phase is: pure methanol and 1% acetic acid water mixture by volume ratio, and the binary gradient is elut...

Embodiment 3

[0035] Weigh chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C reference substances 10, 10, 10, 40, 10 mg respectively, and dilute to 10 mL with methanol. Then take 0.5, 0.2, 0.5, 0.25, 4 mL respectively and dilute to 10 mL with methanol as the standard mixture, and analyze by HPLC.

[0036] Take a certain amount of eucalyptus-type propolis for testing, freeze and pulverize and mix, take 0.25 g, and use 90% ethanol for ultrasonic extraction, the ratio of solid to liquid is 1:30, ultrasonic for 30 min, extract twice, and use 90% ethanol to volume, After filtering through a microporous membrane, it is determined by high performance liquid chromatography.

[0037] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL.

[0038] The mobile phase is: pure methanol and 1% acetic acid water mixture by volume ratio, and the binary gradient ...

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Abstract

The invention provides a method for controlling the quality of Dionysus type propolis, which uses chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C as reference indicators, and uses high-performance liquid chromatography to detect chlorogenic acid in propolis. Ortho acid, p-coumaric acid, caffeic acid, rutin and Artepillin C, truly realize the fidelity and anti-counterfeiting of Dionysus propolis. The method of the present invention has the advantages of high accuracy, high sensitivity, short relative time, low cost and the like in identifying the authenticity of the Dionysus-type propolis, and solves the technical problems of authenticity identification and quality control of the Dionysus-type propolis. It can qualitatively identify Dionysus-type propolis and effectively identify whether rutin is added, which is an effective method for authenticity and de-falsification of Dionysus-type propolis.

Description

technical field [0001] The invention belongs to the field of bee products and relates to a method for quality control of Dionysus type propolis. Background technique [0002] Propolis has a wide range of biological activities, mainly antibacterial, antifungal, antiprotozoal, antiviral, antitumor, immunomodulatory, anticancer and antioxidant activities. Propolis has been widely used in health food, beverage and medicine. [0003] Brazil is located in the Amazon Basin of South America. It has the largest virgin forest in the world, and its plant species ranks first in the world. It is known as the "treasure house of plants" in the world. The chemical composition and pharmacological activity of propolis from different geographical sources in Brazil are quite different. Current research shows that propolis in Brazil can be divided into 13 types according to geographical origin, chemical composition and plant source, among which green propolis in southeast Brazil is the most re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 张翠平胡福良黄帅刘嘉
Owner ZHEJIANG UNIV
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