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Preparation method of temperature and fluorescence probe of lipidosome loaded with gold nanocluster and anti-cancer drug

A technology of anticancer drugs and gold nanoclusters, which is applied in liposome delivery, drug combination, antineoplastic drugs, etc., can solve the problems of liposomes that have not been seen yet, and achieve the effect of low cost and simple method

Inactive Publication Date: 2014-02-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no Chinese patent reports on liposomes loaded with photothermosensitive fluorescent nanomaterials, loaded with fluorescent gold nanoclusters and anticancer drugs, and temperature fluorescent probes based on liposome drug carriers

Method used

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  • Preparation method of temperature and fluorescence probe of lipidosome loaded with gold nanocluster and anti-cancer drug
  • Preparation method of temperature and fluorescence probe of lipidosome loaded with gold nanocluster and anti-cancer drug
  • Preparation method of temperature and fluorescence probe of lipidosome loaded with gold nanocluster and anti-cancer drug

Examples

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Effect test

Embodiment 1

[0021] The preparation method of the liposome temperature fluorescent probe loaded with gold nanoclusters and anticancer drugs, the detailed preparation steps are as follows: Weigh 50mmol dilipoic acid and add it to 20mL deionized water, adjust to pH8.0 with sodium hydroxide, and then Add 2.5mmol of chloroauric acid and 5mmol of sodium borohydride, and stir to mix evenly. Then transfer to a microwave reactor and react at 180W power for 5min. After the reaction, the product is cooled to room temperature 20°C, centrifuged, and filtered with a 15kDa shear molecular weight filter to obtain a precipitate and disperse it in 1mM phosphate buffer. (pH7.4), the dispersion liquid is the gold nanocluster dispersion liquid, which is stored at 4°C or used for subsequent experiments.

[0022] Weigh 3mmol lecithin and 1mmol cholesterol and dissolve in a mixed solvent consisting of 15mL chloroform and 5mL methanol, stir magnetically to form a homogeneous solution, and store at 37°C and N 2 R...

Embodiment 2

[0025] Weigh 50mmol of dilipoic acid and add it into 20mL of deionized water, adjust the pH to 8.5 with sodium hydroxide, add 5mmol of chloroauric acid and 5mmol of sodium borohydride in sequence, and mix evenly with magnetic stirring. Then transfer to a microwave reactor and react at 200W power for 5min. After the reaction, the product is cooled to room temperature 20°C, centrifuged, and filtered with a filter membrane with a shear molecular weight of 15kDa to obtain a precipitate and disperse it in 1mM phosphate buffer. Medium (pH7.4), the dispersion liquid is the gold nanocluster dispersion liquid, which is stored at 4°C or used for subsequent experiments.

[0026] Weigh 4mmol lecithin and 1mmol cholesterol and dissolve in a mixed solvent consisting of 20mL chloroform and 5mL methanol, stir magnetically to form a homogeneous solution, and store at 37°C and N 2 Rotary evaporate the solvent under the atmosphere to obtain a thin layer of phospholipid film, dry it in vacuum to ...

Embodiment 3

[0028] Weigh 50mmol of dilipoic acid and add it to 20mL of deionized water, adjust the pH to 9.0 with sodium hydroxide, add 1mmol of chloroauric acid and 2mmol of sodium borohydride in sequence, and mix evenly with magnetic stirring. Then transfer to a microwave reactor and react at 200W power for 3min. After the reaction, the product is cooled to room temperature 20°C, centrifuged, and filtered with a filter membrane with a shear molecular weight of 10kDa to obtain a precipitate and disperse it in 1mM phosphate buffer. Medium (pH7.4), the dispersion liquid is the gold nanocluster dispersion liquid, which is stored at 4°C or used for subsequent experiments.

[0029] Weigh 5mmol of lecithin and 1mmol of cholesterol and dissolve in a mixed solvent consisting of 25mL of chloroform and 5mL of methanol, and magnetically stir to form a homogeneous solution. 2 Rotary evaporate the solvent under the atmosphere to obtain a thin layer of phospholipid film, dry it in vacuum to make a dry...

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Abstract

The invention relates to a preparation method of a temperature and fluorescence probe of lipidosome loaded with a gold nanocluster and an anti-cancer drug. The method comprises the following steps of: 1) preparing a near-infrared emitting fluorescent gold nanocluster through the microwave pyrolysis method in an aqueous phase way by using dithio-octanoic acid as a protective agent, chloroauric acid as a raw material and sodium borohydride as a reducing agent; 2) dissolving lecithin and cholesterol with a mixed solvent consisting of chloroform and methyl alcohol, carrying out rotary evaporation to remove the solvent to obtain a thin and dried phospholipid membrane, dissolving with gold nanocluster turbid liquid and an anti-cancer drug aqueous solution, and then incubating by the supercritical carbon dioxide method to prepare lipidosome turbid liquid with the gold nanocluster and the anti-cancer drug loaded in an inner water phase. Compared with the prior art, the method has the advantages of being simple and relatively low in cost; the prepared lipidosome drug carrier has light-induced heat sensitivity and fluorescence intensity heat sensitivity, and a novel temperature and fluorescence probe on the basis of the lipidosome drug carrier can be developed, so that important reference value is brought for preparation and application of other drug carriers.

Description

technical field [0001] The invention belongs to the technical field of biomaterials and nanometer medicine, and in particular relates to a preparation method of a liposome temperature fluorescent probe loaded with gold nanoclusters and anticancer drugs. Background technique [0002] Liposome (liposome) refers to a closed vesicle composed of phospholipids as a membrane material and an auxiliary agent. It has a bilayer structure similar to a biological membrane and can encapsulate water-soluble or fat-soluble drugs in the capsule to become a kind of Novel drug carrier. The basic component of liposome is phospholipid, which is an inherent component of organisms, can be degraded by biotransformation in vivo, and has no toxicity and immunogenicity. In view of the fact that liposomes have many advantages such as improving drug stability, reducing drug dosage, reducing toxicity, alleviating immune response, delaying release, reducing in vivo digestion speed, and changing drug dist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/02A61K41/00A61P35/00A61K31/337A61K31/704
Inventor 万锕俊桂日军
Owner SHANGHAI JIAO TONG UNIV
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