Detection method for preparation containing ganoderan
A technology of Ganoderma lucidum polysaccharide and detection method, which is applied in the preparation of test samples, measurement of color/spectral properties, etc., can solve problems such as poor specificity, irrationality, and unscientificity, and achieve easy operation, accurate content and low detection cost. Effect
Active Publication Date: 2014-03-05
培力(南宁)药业有限公司
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Problems solved by technology
In foreign countries, the quality control of polysaccharides mostly uses high performance gel chromatography to determine the molecular weight and distribution of polysaccharides. This is also one of the quality control methods for polysaccharide products. It is better for the quality control of polysaccharide products with higher purity, and can effectively evaluate the polysaccharide. However, there are few quality control methods for Ganoderma lucidum polysaccharide preparations mainly containing polysaccharides. Since the effective polysaccharides contained in them are a large class, it is difficult to evaluate the medicinal materials and their polysaccharide content and molecular weight distribution range. The authenticity and pros and cons of the quality of its preparations, this evaluation method cannot reflect their overall internal quality changes, there is a certain irrationality and unscientific nature, and the specificity is also poor
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Embodiment 1
[0063] Pharmaceutical preparation containing Ganoderma lucidum polysaccharide, precision dextran (MW=2.98×10 5 g / mol) standard 25mg, add water to dissolve to 100ml, 0.25mg dextran per 1ml solution, accurately draw 0, 0.2, 0.4, 0.8, 1.0, 1.5ml dextran standard solution and place in 25ml colorimetric In the tube, add water to 2.0ml accurately, add 1ml of 5% phenol solution, mix well, add 5ml concentrated sulfuric acid, mix well, put the colorimetric tube in a 90°C water bath (the thermometer in the beaker containing the colorimetric tube shows 90°C) Incubate for 15 minutes (15 minutes is strictly required), take it out and cool it at room temperature for 15 minutes, use a spectrophotometer at 490nm to measure the absorbance value with a blank reagent as a reference, and a 1cm cuvette. Draw the standard curve with the concentration of dextran (mg / ml) as the abscissa and the absorbance A as the ordinate. standard curve as figure 1 mentioned.
[0064] Preparation of blank reage...
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The invention discloses a detection method for a preparation containing ganoderan. The detection method is characterized by comprising the following steps: carrying out alcohol precipitation extraction on crude polysaccharides of a preparation; purifying by utilizing alkaline copper sulfate and then developing by phenol sulfuric acid; and determining the ganoderan of the preparation by a colorimetric method of an ultraviolet-visible spectrophotometry. The detection method has the advantages that the ganoderan in the preparation is selectively precipitated by adopting a specific reagent so that the ganoderan is separated from polysaccharides in dextrin; the interferences of auxiliary materials are eliminated and the aim of accurately detecting the content of the ganoderan is realized. The method is simple and accurate; the reagent is easily available and the detection cost is low; the detection method can be used for detecting the preparation containing the ganoderan.
Description
technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations, and relates to a detection method of preparations containing ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum is Ganoderma lucidum (Leyss.ex Fr.) Karst. or Zizhi G. sinense Zhao, the dried fruiting body of Xu et Zhang, has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. Ganoderma lucidum polysaccharide is one of the main medicinal ingredients in the medicinal fungus Ganoderma lucidum. Modern pharmacological studies have shown that Ganoderma lucidum polysaccharide has significant activities such as improving immunity, anti-tumor, scavenging free radicals, and anti-aging. There are more than 200 kinds of Ganoderma lucidum polysaccharides that have been isolated, most of which are β-glucans, and a few are α-glucans. The polysaccharide chain is composed of three strands of monosaccharide chains, whi...
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IPC IPC(8): G01N21/31G01N21/33G01N1/34
Inventor 林广黄志权王德芳周永强倪夕科
Owner 培力(南宁)药业有限公司