Method for measuring daily mean stable carbon isotope composition of atmospheric carbon dioxide

A technology for stabilizing carbon isotopes and carbon dioxide, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficulty in obtaining stable carbon isotope composition values, measurement errors, etc., and achieves the effect of fewer steps, simple calculation, and faster speed.

Active Publication Date: 2014-03-05
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

Due to the complexity of the gas in the region to be measured and the variability over time, it is difficult to obtain the stable carbon isotope composition values ​​of atmospheric carbon dioxide with regional characteristics; only values ​​at certain time points can be obtained, and these values ​​are due to the gas The complexity brings a certain degree of measurement error

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  • Method for measuring daily mean stable carbon isotope composition of atmospheric carbon dioxide

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example

[0045] Example of the present invention: it comprises the following steps:

[0046] First, determine the sodium bicarbonate produced by different manufacturers, choose two kinds of δ 13 Sodium bicarbonate whose C value difference is greater than 10‰ is used as a tracer for isotope label 1 and isotope label 2;

[0047] Second, the tracers of isotope label 1 and isotope label 2 were added to the nutrient solution respectively, the concentration of sodium bicarbonate in the nutrient solution was set to 10 mM, the pH was 8.30, and the bicarbonate ion δ in the nutrient solution of isotope label 1 13 C value is δ C1 , bicarbonate ion δ in the nutrient solution labeled with isotope 2 13 C value is δ C2 ;

[0048] Thirdly, the above-prepared nutrient solution is used to simultaneously cultivate plants with the same growth cycle in the environment to be tested. After 24 hours of cultivation, the stable carbon isotope composition δ in the two isotope-labeled nutrient solutions is me...

Embodiment 1

[0053] First, determine the sodium bicarbonate produced by different manufacturers, choose two kinds of δ 13 Sodium bicarbonate whose C value difference is greater than 10‰ is used as a tracer for isotope label 1 and isotope label 2;

[0054] Second, the tracers of isotope label 1 and isotope label 2 were added to the Hoagland nutrient solution respectively. The concentration of sodium bicarbonate in the nutrient solution was set at 10 mM, and the pH was 8.30. δ 13 C value is δ C1 , bicarbonate ion δ in the nutrient solution labeled with isotope 2 13 C value is δ C2 ;

[0055] Thirdly, the above-prepared nutrient solution was used to simultaneously cultivate Zhuge Cai with the same growth cycle in the test environment 1. After culturing for 24 hours, the stable carbon isotope composition δ in the two isotope-labeled Hoagland nutrient solutions were measured respectively. 13 C value, denoted as δ 1 and δ 2 value;

[0056] Fourth, the measured δ C1 ,δ C2 ,δ 1 and δ 2...

Embodiment 2

[0060] First, determine the sodium bicarbonate produced by different manufacturers, choose two kinds of δ 13 Sodium bicarbonate whose C value difference is greater than 10‰ is used as a tracer for isotope label 1 and isotope label 2;

[0061] Second, the tracers of isotope label 1 and isotope label 2 were added to the Hoagland nutrient solution respectively. The concentration of sodium bicarbonate in the nutrient solution was set at 10 mM, and the pH was 8.30. δ 13 C value is δ C1 , bicarbonate ion δ in the nutrient solution labeled with isotope 2 13 C value is δ C2 ;

[0062] Thirdly, the above-prepared nutrient solution was used to simultaneously cultivate Brassica napus with the same growth cycle in the test environment 2. After culturing for 24 hours, the stable carbon isotope composition δ in the two isotope-labeled Hoagland nutrient solutions were measured respectively. 13 C value, denoted as δ 1 and δ 2 value;

[0063] Fourth, the measured δ C1 ,δ C2 ,δ 1 and...

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Abstract

The invention discloses a method for measuring the daily mean stable carbon isotope composition of atmospheric carbon dioxide, which is characterized by comprising the steps of (I) measuring sodium bicarbonate produced by different manufacturers as tracer agents; (II) adding sodium bicarbonate into a nutrient solution respectively, and setting the concentration of sodium bicarbonate in the nutrient solution to 10mM and the pH value to 8.30; (III) cultivating the plants with same growth cycle by use of the prepared solution at the same time, and after 24 hours of cultivation, separately measuring the values of the stable carbon isotope composition (delta)13C in two isotope-labeled nutrition solutions, namely a value of (delta)1 and a value of (delta)2; (IV) substituting the measured values of (delta)C1, (delta)C2, (delta)1 and (delta)2 into an equation, and calculating the proportion fB of added sodium bicarbonate to total inorganic carbon in the solution; (V) judging whether the value of fB is less than 0.6, fetching the measured values obtained by an experiment when the value of fB is less than 0.60, substituting the measured values into an equation, and calculating the average value of inorganic carbon (delta)13C entering the culture solution in atmospheric carbon dioxide within the time period (delta)a; (VI) substituting the calculated (delta)a into an equation (delta)Ca=(delta)a+(Delta)CO2(air)-HCO3(aq), and calculating the daily mean stable carbon isotope composition (delta)Ca of atmospheric carbon dioxide.

Description

technical field [0001] The invention relates to a method for measuring the daily average stable carbon isotope composition of atmospheric carbon dioxide, which belongs to the field of ecological environment system monitoring, control and restoration. Background technique [0002] From the beginning of the Industrial Revolution to the present, CO 2 Concentration increased by 31%. There is strong evidence that these increases are largely due to the burning of fossil fuels for human activities such as transportation, heating, and electricity generation. by CO 2 The resulting increase in the greenhouse effect accounts for two-thirds of the current increase in the greenhouse effect. Between 10,000 and 250 years ago, atmospheric CO 2 The concentration is very stable, maintained between 260 ~ 280ppmv. CO over the past 250 years 2 Concentrations increased to 370 ppmv, with most of the increase occurring in recent decades. Many factors clearly show that human activities are th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 吴沿友杭红涛谢腾祥王瑞陆叶张开艳姚凯刘莹刘丛强王宝利
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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