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Enzyme label antibody reagent

A technology of enzyme-labeled antibodies and reagents, which is used in measuring devices, instruments, scientific instruments, etc., to achieve the effects of good stability, saving reaction time and long-term application.

Active Publication Date: 2014-03-05
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Whether it is the glutaraldehyde two-step method or the simple sodium periodate method, it is necessary to purify the enzyme conjugate to remove free antibody and horseradish peroxide Enzyme, the operation steps are complicated and cumbersome, and the enzyme conjugate is easily lost during the purification process, which increases the cost and increases the preparation time
At the same time, the reagents used for the Meibiao antibody need to be prepared and used immediately, and cannot be stored for a long time, which limits the preparation of enzyme-labeled substances in the enzyme-linked immunoassay

Method used

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  • Enzyme label antibody reagent
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The enzyme-labeled antibody reagent components and concentrations were

[0027] Component 1 (R1):

[0028] Acetate buffer solution with pH 6.0 1mmol / L

[0029] Sodium Periodate 0.05mol / L

[0030] Carbamide peroxide 0.5g / L

[0031] Triethanolamine 0.1mL / L

[0032] Prepared by dissolving in distilled water;

[0033] Component 2 (R2):

[0034] Anhydrous sodium carbonate 63.6 g / L

[0035] Sodium bicarbonate 33.6g / L

[0036] Prepared by dissolving in distilled water;

[0037] Component 3 (R3):

[0038] Sodium borohydride 4g / L

[0039] Glycine 2 g / L

[0040] Glycerin 1mL / L

[0041] Dissolve glycerin in distilled water, then add glycine and sodium borohydride to dissolve.

[0042] The application process of the enzyme-labeled antibody reagent is as follows: Weigh 5 mg of HRP and dissolve it in 1 mL of R1 component, incubate at 37°C for 60 minutes, add 200 μL of R2 component, and add 10 mg of antibody to be labeled at the same time, incubate at 37°C for...

Embodiment 2

[0043] Example 2: The enzyme-labeled antibody reagent components and concentrations were

[0044] Component 1 (R1):

[0045] Acetate buffer solution with pH 6.0 5mmol / L

[0046] Sodium Periodate 0.1mol / L

[0047] Carbamide peroxide 0.5g / L

[0048] Triethanolamine 1mL / L

[0049] Prepared by dissolving in distilled water;

[0050] Component 2 (R2):

[0051] Anhydrous sodium carbonate 63.6 g / L

[0052] Sodium bicarbonate 33.6g / L

[0053] Prepared by dissolving in distilled water;

[0054] Component 3 (R3):

[0055] Sodium borohydride 4g / L

[0056] Glycine 2 g / L

[0057] Glycerin 1mL / L

[0058] Dissolve glycerin in distilled water, then add glycine and sodium borohydride to dissolve.

[0059] Using method is the same as embodiment 1.

Embodiment 3

[0060] Example 3: The enzyme-labeled antibody reagent components and concentrations were

[0061] Component 1 (R1):

[0062] Acetate buffer solution with pH 6.0 10mmol / L

[0063] Sodium Periodate 0.5mol / L

[0064] Carbamide peroxide 1g / L

[0065] Triethanolamine 1mL / L

[0066] Prepared by dissolving in distilled water;

[0067] Component 2 (R2):

[0068] Anhydrous sodium carbonate 63.6 g / L

[0069] Sodium bicarbonate 33.6g / L

[0070] Prepared by dissolving in distilled water;

[0071] Component 3 (R3):

[0072] Sodium borohydride 4g / L

[0073] Glycine 2 g / L

[0074] Glycerin 1mL / L

[0075] Dissolve glycerin in distilled water, then add glycine and sodium borohydride to dissolve.

[0076] Using method is the same as embodiment 1.

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Abstract

The invention relates to the technical field of clinical in-vitro detection reagents, and in particular relates to an enzyme label antibody reagent which comprises a component 1, a component 2 and a component 3, wherein the component 1 contains an acetate buffer solution with the pH value being 6.0, sodium periodate, urea peroxide and triethanolamine; the component 2 contains anhydrous sodium carbonate and sodium bicarbonate; the component 3 contains sodium borohydride, glycine and glycerinum. The enzyme label antibody reagent is used for directly killing dissociative antibodies and HRP (Hypothalamic Regulatory Peptides) without the needs of dialyzing and purifying enzyme conjugates, and can be directly applied into a kit without influencing background and detection results. As the enzyme label antibody reagent is used, the conventional label process passing the night (18 hours) is shortened to be finished by 2 hours, so that the reaction time is greatly saved. If the enzyme label antibody reagent is placed in an environments at 2 DEG C to 8 DEG C as well as 37 DEG C, the enzyme label antibody reagent can be stably stored for a long time, thereby having the good stability. As a result, the enzyme label antibody reagent can be used for a long time.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an enzyme-labeled antibody reagent. Background technique [0002] In 1971, Engvall and Perlmann published an article on the use of enzyme-linked immunosorbent assay (enzyme linked immunosorbent assay, elisA) for the quantitative determination of IgG, which made the enzyme-labeled antibody technology for antigen localization developed in 1966 into the detection of trace substances in liquid specimens. test methods. The basic principle of this method is: ① Make the antigen or antibody bind to the surface of a certain solid phase carrier and maintain its immunological activity. ②The antigen or antibody is linked with horseradish peroxidase (HRP) to form an enzyme-labeled antigen or antibody, which not only retains its immune activity, but also retains the activity of the enzyme. During the determination, the test specimen (determining the antibody or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/535
Inventor 谭柏清王进甘宜梧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD