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Rapid induction method for inserting adventitious bud on bean halve green leaf

An induction method, green leaf technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long time for adventitious buds, difficulty in forming new plants, and small number of adventitious buds, so as to improve reproductive efficiency and Commodity flower quality, solving the effect of long occurrence time and avoiding root rot

Inactive Publication Date: 2014-03-19
CHANGCHUN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Aiming at the deficiencies and defects of the prior art, the purpose of the present invention is to provide a method for promoting rapid induction of adventitious buds during the propagation of watercress green leaf cuttings, to solve the problem that after the watercress green leaf cuttings take root, the occurrence of adventitious buds takes a long time, it is difficult to form new plants, and the adventitious buds The problem of small quantity occurs, and the propagation efficiency of watercress green leaf cuttings and the quality of commercial flowers are improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Gently break off the watercress green leaves from the mother plant from top to bottom, dip the incision at the end of the petiole with plant ash and insert it in the KMO 4 In the sterilized humus soil; beat Dryopteris crassirhizoma Nakai into juice with a juicer and let it stand for 5 minutes, then take the supernatant for later use; stack 4 layers of medicinal gauze and put it on the extracted rough After soaking in the sap of Trichofern spp., spread it on the back of the cutting leaves, then fix it with clips, and then cover the leaves with plastic bags; put the test materials in a constant temperature and humidity box to cultivate, and control the soil humidity at about 30%. The air humidity is 50%; the culture temperature is set at a high temperature of 27 ° C for 14 hours a day, and a low temperature of 22 ° C for 10 hours to promote the occurrence of callus; after 7 days, the gauze coated on the back of the leaves is again made of thick stem bulbs. Soak fern super...

Embodiment 2

[0032] Use scissors to cut off the mature watercress green leaves close to the stem, dip the incision at the end of the petiole with plant ash and insert it in the KMO 4 In the sterilized peat soil; use a juicer to make the juice of Trichofern chrysalis and let it stand for 7 minutes, then take the supernatant for later use; stack 3 layers of medicinal gauze and put it in the extracted juice of Trichofern chrysogenum After soaking, spread it on the back of the cuttings leaves, then fix them with clips, and then cover the leaves with plastic bags; put the test materials in a constant temperature and humidity box for cultivation, control the soil humidity at about 40%, and the air humidity at 60%; The temperature is set at a high temperature of 25°C for 16 hours a day, and a low temperature of 15°C for 8 hours; after 5 days, the gauze covered on the back of the leaves is soaked with the prepared supernatant of D. After cutting for 12 days, a new root with a length of 1 cm grows ...

Embodiment 3

[0035] Use scissors to stick the green leaves of mature watercress to the stem and cut them off obliquely, dip the incision at the end of the petiole with plant ash and insert it in KMO 4 In the sterilized river sand; use a juicer to make the juice of Trichofern chrysalis and let it stand for 10 minutes, take the supernatant for later use; stack 5 layers of medicinal gauze and put it in the extracted Juice of Trichofern chrysogenum After soaking, spread it on the back of the cuttings leaves, then fix them with clips, and then cover the leaves with plastic bags; put the test materials in a constant temperature and humidity box for cultivation, control the soil humidity at about 50%, and the air humidity at 70%; The temperature is set at a high temperature of 30°C for 8 hours and a low temperature of 20°C for 16 hours; after 4 days, the gauze applied on the back of the leaves is soaked with the supernatant of D. ; After 12 days of cuttings, new roots with a length of 1 cm grow f...

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PUM

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Abstract

The invention provides a rapid induction method for inserting an adventitious bud on a bean halve green leaf and belongs to the technical field of flower propagation. The rapid induction method comprises the following steps: dipping plant ash on a notch at the end of a bean halve green leaf stalk and inserting the bean halve green leaf stalk into humus disinfected by KMO4; pulping rough stem dryopteris erythrosora into juice and standing; taking liquid supernatant for later use; overlapping three to five layers of medical gauzes; immersing the medical gauzes into the extracted rough stem dryopteris erythrosora juice and paving the medical gauzes on the back face of a cutting slip leaf; covering the leave by using a plastic bag; controlling the soil humidity, the air humidity and the temperature to avoid rotting cutting slips to promote callusogenesis; after cutting for 10-15 days, removing the gauzes and the plastic bag after a new root grows for 1cm on a cutting slip leaf stalk; and irrigating the soil at the root of the cutting slips by the prepared rough stem dryopteris erythrosora juice every 3-5 days to improve the soil humidity to 40%-60% and improve the temperature to be about 25 DEG C. According to the rapid induction method, the problems that the generation time of the adventitious bud in the cuttage process of the bean halve green leaf is long and the quantity is small are solved; the cuttage efficiency of the bean halve green leaf and the quality of commercial flowers is improved.

Description

technical field [0001] The invention belongs to the technical field of flower propagation, and in particular relates to a method for rapidly inducing adventitious buds during the promotion of watercress green leaf cutting propagation. Background technique [0002] Peperomia obtusifolia, also known as green leaf jasper and pepper grass, belongs to the genus Peperomia of the family Piperaceae, and is a perennial evergreen succulent herb suitable for indoor viewing. It is difficult to obtain seeds of watercress green, and the propagation methods that have been researched and applied are mainly cutting propagation and tissue culture. [0003] Tissue culture is mainly used for laboratory research because of its high production cost and complicated operation, but is rarely used by flower growers in production. At present, the most widely used in production is still to use its leaves for cutting propagation. Search "Patent Search System of the State Intellectual Property Office",...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 丁云峰马艳丽高巍周秀华尹立辉谢修鸿王晓红武术杰
Owner CHANGCHUN UNIV
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