Gentianella acuta extract for treating arrhythmia and preparation method and application thereof

A technique for treating arrhythmia with Pseudo gentiana lanceolata and arrhythmia, which is applied in the field of Chinese herbal medicine active ingredient extract, Pseudo gentiana serrata (Hulten) for treating arrhythmia

Active Publication Date: 2014-03-19
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the total effective rate of antiarrhythmic drugs is only 30% to 60%, and it is not effective for some intractable arrhythmias

Method used

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  • Gentianella acuta extract for treating arrhythmia and preparation method and application thereof
  • Gentianella acuta extract for treating arrhythmia and preparation method and application thereof
  • Gentianella acuta extract for treating arrhythmia and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 5000g of the raw material of Pseudomonas gentiana, first reflux extraction with 75% ethanol 20 times the weight of the raw material for 3 times, each extraction for 1.5 hours, combine the above extracts, concentrate under reduced pressure to a paste, and then suspend in water to obtain a suspension liquid medicine.

[0030] After suspending the obtained suspension medicinal solution with water, load it to D101 type macroporous adsorption resin column chromatography (resin weight 80g, column volume 100ml, column diameter: column height=1: 2) for adsorption, and the sample loading flow rate is per hour 1.2 times the column volume, rinse the column with distilled water after loading the sample until the water eluent is nearly colorless, discard the water eluate, and use 6 times the column volume to elute with 60% ethanol aqueous solution, and the flow rate of the eluent is controlled at 0.5 times the column volume per hour, during the elution process, use TCL to detec...

Embodiment 2

[0032] Take 5000g of the raw material of Pseudomonas gentiana, first reflux extraction with 75% ethanol 20 times the weight of the raw material for 3 times, each extraction for 1.5 hours, combine the above extracts, concentrate under reduced pressure to a paste, and then suspend in water to obtain a suspension liquid medicine.

[0033] After the suspending medicinal liquid of gained is suspended with water, load sample to AB-8 type macroporous adsorption resin column chromatography (resin weighs 80g, and column volume 100ml, column diameter: column height=1: 2) is adsorbed, and sample loading flow rate is 1.2 times the column volume per hour, wash the column with distilled water after loading the sample, until the water eluent is nearly colorless, discard the water eluate, and elute with 6 times the column volume containing 60% ethanol aqueous solution, the flow rate of the eluent is The column volume is controlled at 0.5 times per hour. During the elution process, TCL is used...

Embodiment 3

[0035] Take 5000g of the raw material of Pseudomonas gentiana, first reflux extraction with 75% ethanol 20 times the weight of the raw material for 3 times, each extraction for 1.5 hours, combine the above extracts, concentrate under reduced pressure to a paste, and then suspend in water to obtain a suspension liquid medicine.

[0036] After suspending the obtained suspension medicinal solution with water, load it to H103 type macroporous adsorption resin column chromatography (resin weight 80g, column volume 100ml, column diameter: column height=1: 2) for adsorption, and the sample loading flow rate is per hour 1.2 times the column volume, rinse the column with distilled water after loading the sample until the water eluent is nearly colorless, discard the water eluate, and use 6 times the column volume to elute with 60% ethanol aqueous solution, and the flow rate of the eluent is controlled at 0.5 times the column volume per hour, during the elution process, use TCL to detec...

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PUM

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Abstract

The invention discloses a gentianella acuta extract for treating arrhythmia. The extract consists of ketone compounds, iridoid compounds and flavonoid compounds. The invention also discloses a preparation method and application of the gentianella acuta extract. The gentianella acuta extract disclosed by the invention comprises 20-40% of ketone compounds based on the total weight of the extract, 5-15% of iridoid compounds based on the total weight and a small amount of flavonoid compounds, wherein the ketone compounds include 1,3,5,8-tetrahydroxy tetrahydro-ketone, nor-chiratin, 3,8-O-beta-D-diglucopyranose-1,5-dihydroxy-keton, nor-bellidifolin, 1,3,5-trihydroxy-ketone, 2-O-beta-D-n glucopyranose-1-hydroxy-5,6-dimethoxy-ketone and 1,2,8-trihydroxy-5,6-dimethoxy-ketone; the iridoid compounds include gentiopicroside, swertiamarin and decentapicrin; the flavonoid compounds include isoorientin and isovitexin. Chemical and pharmacological experiments prove that the gentianella acuta extract can be used for treating arrhythmia caused by coronary heart disease and ischemic heart disease. The gentianella acuta extract can be made into drugs of various dosage forms independently or by compatible combination or can be used as a raw medicine.

Description

technical field [0001] The invention relates to an extract of active ingredients of Chinese herbal medicine, in particular to an extract of Gentianella acuta (Michx.) Hulten for treating arrhythmia, a preparation method and a medical application thereof, and belongs to the field of extracts of effective parts of traditional Chinese medicine . Background technique [0002] Gentianella acuta (Michx.) Hulten, also called Gentianella acuta (Michx.) Hulten, is an annual herb of the genus Gentianaceae. provinces. The whole herb is used in Mongolian medicine. It tastes bitter and cool in nature. It has the effect of clearing away heat and dampness. It is the main medicine used in Mongolian medicine to treat jaundice, headache, fever, and dry mouth. It is used by hunters of the Orenchun ethnic group in the Greater Khingan Mountains to treat arrhythmia and other diseases. ,Significant effect. The main chemical components contained in the plant reported in the literature are: Ket...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/515A61P9/06A61P9/10
Inventor 匡海学王知斌王秋红于莹杨炳友翟亚东宋璞原薛娟
Owner 匡海学
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