Application of swertianolin in preparation of medicine used for preventing and treating arrhythmia

A technology for drug alcohol glycosides and arrhythmia, which is applied in drug combinations, pharmaceutical formulas, plant raw materials, etc.

Active Publication Date: 2014-01-29
李旻辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no literature report on the effect of swertianolin on the prevention and treatment of arrhythmia

Method used

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  • Application of swertianolin in preparation of medicine used for preventing and treating arrhythmia
  • Application of swertianolin in preparation of medicine used for preventing and treating arrhythmia
  • Application of swertianolin in preparation of medicine used for preventing and treating arrhythmia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Study on the Pharmacological Actions of Swertianolin, a Monomer Compound in Pseudogentiana Acuminate

[0024] 1 Materials and methods

[0025] 1.1 Animals Male KM mice, weighing 18-22 g, were provided by the Experimental Animal Center of Inner Mongolia University.

[0026] 1.2 Drugs Alcohol Glycosides (self-made, purity greater than 98% by HPLC); Lidocaine Injection 5mg / bottle (batch number 1006182, Hebei Tiancheng Pharmaceutical Co., Ltd.); aconitine (batch number 110819, Weiqiqi, Sichuan Province Biotechnology Co., Ltd.); urethane (Wuhan Jinnuo Chemical Co., Ltd.).

[0027] 1.3 Instruments BL-420F biological function experiment system, Chengdu Taimeng Technology Co., Ltd., China.

[0028] 1.4 Method

[0029] Eighty healthy male KM mice were randomly divided into 5 groups (blank control group, lidocaine control group, test drug high, medium and low dose groups), 16 in each group. The blank control group was intragastrically given distilled water (0.1mL / 10g); the li...

Embodiment 2

[0046] Preparation method of drug alcohol glycoside compound and its extract

[0047] 1 Materials and methods

[0048] 1.1 Materials Pseudogentiana acuminate was collected from Genhe City, Inner Mongolia Autonomous Region in July 2008. The sample was identified as Gentianaceae by Mr. Wang Zhenwang from the Teaching and Research Office of Medicinal Plants, Baotou Medical College; thin-layer silica gel G was purchased from Qingdao Ocean Chemical Plant; dextran gel (Sephadex LH-20) was purchased from GE Company of the United States; reagents (petroleum ether, ethyl acetate, n-butanol, ethanol, methanol) were of analytical grade.

[0049] 1.2 Extraction of drug alcohol glycosides

[0050] Weigh 1 kg of the crushed and dried aerial part of Pseudomonas cuspidatum, reflux extract with 70% ethanol for 3 times, each time for 2 hours, combine the extracts and concentrate to extract, and obtain 230 g of extract. Dissolve the obtained extract in 1000mL water, extract with petroleum ethe...

Embodiment 3

[0057] (1) Crush and dry the above-ground part of Pseudomonas acuminate, reflux extract with 70% ethanol for 3 times, combine the extracts and concentrate to extract. The obtained extract was dissolved in water and extracted with petroleum ether (60-90°C), ethyl acetate and n-butanol respectively. The n-butanol extract was taken, and the solvent was recovered under reduced pressure to obtain a partial n-butanol extract.

[0058] (2) Take part of the n-butanol extract, dissolve and filter with 20 times the amount of methanol, then pack it into a column with Sephadex LH-20 (column size 8cm×80cm), wash with 70% methanol as mobile phase take off. Combine the fractions containing glucosides, and recover the solvent under reduced pressure to obtain an extract containing 82.5% glycosides.

[0059] (3) Purify the alcoholic glycoside extract of Dangdao with Sephadex LH-20 (column size 3cm×100cm), elute with 80% methanol, collect the fractions and recover the solvent under reduced pre...

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Abstract

The invention relates to an application of swertianolin (chemical name is 1,5,8-trihydroxy-3-methoxy xanthone-8-oxy glucoside, swertianolin) shown as a formula (I) and extract thereof in preparation of a medicine used for preventing and treating arrhythmia. The invention also discloses a pharmaceutical composition containing the swertianolin or a pharmaceutically acceptable carrier, excipient or diluter thereof as an active ingredient. Pharmacological experimental study results show that a swertianolin compound and an extract thereof have an antiarrhythmic effect, can obviously reduce atrioventricular block and ventricular tachycardia which are caused by aconitine and can reduce activity of blood serum myocardial enzyme during arrhythmia; and the swertianolin compound has similar pharmacological properties compared with a positive control lidocaine group. The study results of the invention provide powerful scientific basis for rational utilization of gentianella acuta Chinese herbal medicine.

Description

technical field [0001] The present invention relates to a new application of the monomer compound swertianolin in Pseudogentiana acuminate, more specifically the application of the swertianolin and its extract in the preparation of drugs for preventing and treating arrhythmia. Background technique [0002] According to the relevant literature of traditional Chinese medicine, the medicinal plants of the genus Pseudogentian often replace some medicinal plants of the genus Swertia and Sternia for the treatment of hepatitis and biliary colic. From the perspective of plant phylogeny, Pseudogentian is closely related to Swertia and Costa; from the perspective of chemical composition, the phytochemical components of this genus are similar to those of Swertia and Costa. There are similarities, and there is a certain scientific reason for the mutual substitution of drugs. Pseudogentian plants have been used in ethnic (Tibetan, Mongolian, etc.) medicines in my country, so in-depth ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K36/51A61P9/06
Inventor 李旻辉吕丽娟闫暾
Owner 李旻辉
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