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Technology for preparing catechin oxidation polymer with steapsase inhibition effect

A technology for inhibiting pancreatic lipase, which is applied in the field of polymer preparation, can solve the problems that the reaction speed is not easy to manually control, and the generation of catechin oxidized polymer is not easy to detect, etc., and achieves the effect of high safety and reliability.

Inactive Publication Date: 2014-03-19
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fermentation of tea leaves is an internal natural fermentation and oxidation process of tea leaves, its reaction speed is not easy to control artificially, and the formation of catechin oxidation polymers is also difficult to detect.

Method used

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  • Technology for preparing catechin oxidation polymer with steapsase inhibition effect
  • Technology for preparing catechin oxidation polymer with steapsase inhibition effect
  • Technology for preparing catechin oxidation polymer with steapsase inhibition effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Add 1g of green tea extract with 98% catechin content and 200ml of distilled water into a beaker with a stirring magnet, and stir evenly with a magnetic stirrer at 21-25°C until it is in a clear and transparent liquid state;

[0014] 2) Add 50g of fresh pear fruit and 300ml of distilled water into the pulverizer, pulverize fully to obtain pulverized pear juice, filter the sediment with four layers of gauze, and obtain pear juice suspension;

[0015] 3) Add the pear juice suspension prepared in step 2) to the solution prepared in step 1), and inject oxygen into the beaker while stirring with a magnetic stirrer so that the pear juice is evenly distributed in the beaker liquid. React at 21-25°C for 3 hours, and confirm that the catechin is completely oxidized and the catechin oxidation polymer is produced by thin layer chromatography (TLC) or high performance liquid chromatography (HPLC);

[0016] 4) After cooling, filtering and concentrating the liquid obtained in step...

Embodiment 2

[0018] 1) Add 2g of green tea extract with 98% catechin content and 400ml of distilled water into a beaker with a stirring magnet, and stir evenly with a magnetic stirrer at 21-25°C until it is in a clear and transparent liquid state;

[0019] 2) Add 100g of fresh pear fruit and 600ml of distilled water into the pulverizer, pulverize fully to obtain pulverized pear juice, filter the sediment with four layers of gauze, and obtain pear juice suspension;

[0020] 3) Add the pear juice suspension prepared in step 2) to the solution prepared in step 1), and inject oxygen into the beaker while stirring with a magnetic stirrer so that the pear juice is evenly distributed in the beaker liquid. React at 21-25°C for 4 hours, and confirm that catechin is completely oxidized and catechin oxidation polymer is produced by thin layer chromatography (TLC) or high performance liquid chromatography (HPLC);

[0021] 4) After cooling, filtering and concentrating the liquid obtained in step 3), gr...

Embodiment 3

[0023] 1) Add 5g of green tea extract with a catechin content of 98% and 1000ml of distilled water into a beaker with a stirring magnet, and stir evenly with a magnetic stirrer at 21-25°C until it is in a clear and transparent liquid state;

[0024] 2) Add 250g of fresh pear fruit and 1000ml of distilled water into the pulverizer, pulverize fully to obtain pulverized pear juice, filter the sediment with four layers of gauze, and obtain pear juice suspension;

[0025] 3) Add the pear juice suspension prepared in step 2) to the solution prepared in step 1), and inject oxygen into the beaker while stirring with a magnetic stirrer so that the pear juice is evenly distributed in the beaker liquid. React at 21-25°C for 5 hours, and confirm that the catechin is completely oxidized and the catechin oxidation polymer is produced by thin layer chromatography (TLC) or high performance liquid chromatography (HPLC);

[0026] 4) After cooling, filtering and concentrating the liquid obtained...

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Abstract

The invention relates to a technology for preparing a catechin oxidation polymer with steapsase inhibition effect, wherein using a biological in vitro enzyme catalytic oxidation model, simulating catalytic oxidation reaction generated when tea fermentation, the technology prepares the catechin oxidation polymer with the steapsase inhibition effect, and comprises the following steps of: preparing a catechin solution, preparing a pear juice turbid liquor, preparing a catechin oxidation polymer, and refining a catechin oxidation polymer. The reaction speed is easy to manually control, the catechin oxidation polymer is easy to detect, and the technology has strong practical value and a market prospect.

Description

technical field [0001] The invention relates to a preparation process of a polymer, in particular to a preparation process for preparing a catechin oxidation polymer with pancreatic lipase inhibitory effect by adopting an in vitro enzyme catalytic oxidation model to simulate the catalytic oxidation reaction occurring during tea fermentation. Background technique [0002] In recent years, with the improvement of living standards and changes in dietary structure and living habits, the number of obese people has been increasing and is on the rise. At present, obesity is a chronic disease that is most easily overlooked by humans, but its incidence is rising sharply. It is closely related to the occurrence and development of many diseases, such as stroke, type II diabetes, hypertension, coronary heart disease, etc. . The root cause of obesity is excessive accumulation of fat in the body. Obesity is a disorder of nutrition and metabolism. At present, most of the obesity is simple...

Claims

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Application Information

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IPC IPC(8): C12P17/16
Inventor 李岩段静雨么焕开崔文李勇艾凤伟
Owner XUZHOU MEDICAL COLLEGE