Deodorant solution and preparation method and preparation kit thereof

A deodorant liquid, Bacillus subtilis technology, applied in deodorization, disinfection and other directions, can solve the problem of high price of activated carbon, and achieve the effects of easy storage, efficient odor removal, and excellent heat resistance

Inactive Publication Date: 2014-03-26
JAPAN ENVIRONMENT DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Activated carbon is expensive, but there is a problem that it must be exchanged frequently

Method used

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  • Deodorant solution and preparation method and preparation kit thereof
  • Deodorant solution and preparation method and preparation kit thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] In the preparation method of the deodorant liquid described in the invention, the 4.0×10 5 more than one / ml, preferably 4.0×10 6 More than one / ml, more preferably 4.0×10 7 piece / m1 or more, more preferably 4.0×l0 8 more than one / ml, and 2.0×10 9 pcs / m1 or less, preferably 1.0×10 9 Bacillus subtilis in an inactive state with a bacterial cell concentration below 7.6 cells / ml and less than 3.8×10 5 cells / ml, heated and activated to obtain a deodorant solution. This stock solution is stable during storage. This stock solution can be diluted with acetate buffer, water, or the like. In general, dilution with tap water is convenient. When activating inactive Bacillus subtilis, germination inducing substances such as amino acids and sugars can also be used.

[0024] The activation condition of Bacillus subtilis in an inactive state is heating at 40°C to 60°C for 20 minutes to 60 minutes, preferably heating at 45°C to 50°C.

[0025] By heating the inactive Bacillus subt...

Embodiment 1

[0029] (Measurement of bacterial cell concentration of Bacillus subtilis contained in Bacillus subtilis solution A)

[0030] The bacterial cell concentration of Bacillus subtilis in the Bacillus subtilis solution A was measured. The Bacillus subtilis liquid A is derived from natural Bacillus subtilis, contains at least Bacillus subtilis, and does not contain bacteria and fungi other than Bacillus subtilis.

[0031] (test methods)

[0032] The bacterial cell concentration of Bacillus subtilis contained in the Bacillus subtilis solution A was measured as follows.

[0033] (1) Under sterile conditions, dilute the Bacillus subtilis solution A to 4,000 times with sterilized water, and repeat the 10-fold dilution of the 0.1% Tween80 solution sterilized by high pressure steam three times to prepare 4 times of the Bacillus subtilis solution A. ×10 4 times, 4×10 5 times and 4×10 6 double step dilution series.

[0034] (2) Transfer 0.5 ml of each step dilution to 3 sterilized petri ...

Embodiment 2

[0040] (Proliferation experiment at each dilution factor of Bacillus subtilis solution A)

[0041] (method)

[0042] (1) For the Bacillus subtilis liquid A used in Example 1, under aseptic conditions, repeat the 10-fold dilution of soybean casein digestion medium (Wako Pure Chemical Industries) sterilized with high pressure steam to prepare 10-10 7 1-fold step dilution series (Experiment 1). Operate in the same way to prepare 10 times, 10 2 times, 10 3 times, 2×10 3 times, 4×10 3 Times, 6×10 3 Times, 8×10 3 times and 10 4 1-fold step dilution series (Experiment 2).

[0043] (2) Each dilution series was heated at 40°C for 1 hour, and then incubated at 35°C for 36 hours. After the cultivation, the turbidity of the culture medium due to the proliferation of bacteria was judged by visual observation.

[0044] (result)

[0045] figure 1 represents the results of Experiment 1. exist figure 1 In the above, when the item of bacterial growth is described as "+", it means ...

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Abstract

The invention provides a deodorant which is safe to human body, avoids degradation of metals and the like and is cheap and high in continuity. The invention provides a deodorant solution in which the cell concentration of bacillus subtilis is higher than 7.6 / ml and lower than 3.8*10<5> / ml. The bacillus subtilis easy to reproduce is adopted in the deodorant solution, and thus mass production can be realized at low price. Moreover, the bacillus subtilis has spore forming property and excellent heat resistance, and thus the deodorant solution and a stock solution for dilution are easy to store. Moreover, the deodorant solution provided by the invention contains bacillus subtilis of which the cell concentration is higher than 7.6 / ml and lower than 3.8*10<5> / ml, can realize quick reproduction of bacillus subtilis and thus can expel the fungi leading to deodorization and effectively eliminate odor.

Description

technical field [0001] The invention relates to a deodorizing liquid used for deodorizing rotten odors, etc., a preparation method and a preparation kit thereof. Background technique [0002] At present, as a method of removing odors such as garbage and feces, there are known methods of converting odor molecules into odorless components through chemical reactions such as oxidation by ozone, or methods of adsorbing odor molecules using activated carbon. . In addition, there is also known a method for deodorizing odor molecules that are the source of bad odor by complexing them with molecules such as cyclodextrin. Contents of the invention [0003] The problem to be solved by the invention [0004] When an oxidizing agent such as ozone is used, if it is used on metal products, it may cause deterioration of the metal products due to rust. In addition, many components that decompose organic malodours, such as putrid odors, are harmful to the human body. Activated carbon is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L9/01A61L101/52
Inventor 本乡洁
Owner JAPAN ENVIRONMENT DEV
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