Antifreezing protective agent for poplar and application method thereof
A technology of antifreeze protection and poplar, applied in the direction of application, fertilizer mixture, fertilization device, etc., can solve the problems of economic loss, lack of good control methods, etc., and achieve the effect of disease control, low cost, and simple application method
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[0012] 1 Materials and methods
[0013] 1.1 Screening of indoor chemicals
[0014] 1.1.1 Test material
[0015] There are 4 kinds of medicaments for indoor screening, namely thiram (40% wettable powder), agricultural streptomycin (10 million units of pure powder), difenazol (10% water), antifreeze protection agent, according to different medicaments Choose 4-6 kinds of concentrations to test. Liquid medium formula: 10 grams of peptone, 4 grams of beef extract, 1 gram of NaCl, and 1000 ml of water. , Add 20 grams of agar to the solid medium on this basis. Tested strain: Pantoea aggiomerans.
[0016] 1.1.2 Test method
[0017] (1) Liquid strain culture: After the prepared liquid medium is autoclaved, strain A is inoculated therein. Then place it in a shaker at 30°C for 48 hours, with a concentration of about 10 9 / ml.
[0018] (2) Drug preparation: According to different drugs, 4 or 6 concentrations were selected for testing, see Table 1. Prepare the medicament accordin...
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