A kind of preparation method of mideamycin
A technology of midecamycin and fermented liquid, which is applied in the field of preparation of high-purity midecamycin, can solve the problems of inability to remove macromolecular soluble proteins, affect product quality and yield, and require a lot of manpower and material resources. White color, simple operation effect
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Embodiment 1
[0025] Adjust the midecamycin fermentation broth to pH 4.0 with hydrochloric acid, and put it into a ceramic membrane filter with a pore size of 0.1 μm. After the filtration is completed, in order to ensure the yield, use 2 times the volume of The purified aqueous solution was topped with water, and the filtrate was collected completely. Take 150L of filtrate, adjust the filtrate to pH 8.5 with sodium hydroxide solution, add 15L of butyl acetate, stir evenly and raise the temperature to 45°C, after standing for stratification, midecamycin is transferred to the upper organic phase, and the lower layer of filtrate is discarded. Add about 3 L of oxalic acid solution with a pH of 2.0 to 2.5, stir evenly and let it stand for stratification, then transfer the midecamycin to the lower aqueous phase, and separate the lower solution. Adjust the phase inversion liquid with sodium hydroxide solution to a pH of 8.0, add 400ml of butyl acetate, and extract at 45°C. After obtaining the extr...
Embodiment 2
[0027] Adjust the midecamycin fermentation broth to pH 5.0 with hydrochloric acid, and put it into a ceramic membrane filter with a pore size of 0.1 μm. After the filtration is completed, in order to ensure the yield, use 2 times the volume of The purified aqueous solution was topped with water, and the filtrate was collected completely. Take 100L of filtrate, adjust the filtrate to pH 9.0 with sodium hydroxide solution, add 15L of butyl acetate, stir evenly and raise the temperature to 50°C, after standing for stratification, midecamycin is transferred to the upper organic phase, and the lower layer of filtrate is discarded. Add 3 L of hydrochloric acid solution with a pH of 2.0 to 2.5, stir evenly and let it stand for stratification, then transfer the midecamycin to the lower aqueous phase, and separate the lower layer solution. Adjust the phase inversion liquid with sodium hydroxide solution to a pH of 8.5, add 450ml of butyl acetate, and extract at 50°C. After obtaining th...
Embodiment 3
[0029] Adjust the midecamycin fermentation broth to pH 4.5 with sulfuric acid, and put it into a ceramic membrane filter with a pore size of 0.1 μm. After the filtration is completed, in order to ensure the yield, use 2 times the volume of The purified aqueous solution was topped with water, and the filtrate was collected completely. Take 500L of filtrate, adjust the filtrate to pH 8.7 with sodium hydroxide solution, add 75L of butyl acetate, stir evenly and raise the temperature to 48°C, after standing for stratification, midecamycin is transferred to the upper organic phase, and the lower layer of filtrate is discarded. Add 15L of sulfuric acid solution with a pH of 2.0 to 2.5, stir evenly and let it stand for stratification, then transfer the midecamycin to the lower aqueous phase, and separate the lower layer solution. Adjust the phase inversion solution with sodium hydroxide solution to a pH of 8.3, add 2000ml of butyl acetate, and extract at 48°C. After obtaining the ext...
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