Composition for processing biological sample

A technology for biological samples and compositions is applied in the field of compositions and liquid media for processing biological samples, and can solve the problems of high production cost, difficult endotoxin control, and obstacles to complex industrial production and transformation.

Active Publication Date: 2014-03-26
BEIJING DONGFANG HUAHUI BIOMEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This shows that the more complex the formula and the more types of main raw materials involved, the more difficult it will be to control endotoxin in the p...

Method used

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  • Composition for processing biological sample
  • Composition for processing biological sample
  • Composition for processing biological sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 General method for preparing composition and liquid medium

[0074] This embodiment provides a method for preparing a composition and liquid medium for processing biological samples, which includes the following steps:

[0075] At a temperature of 20-25°C and a humidity of less than 60%, use a one-ten thousandth precision balance to accurately weigh the solid or liquid raw materials of the corresponding mass, and place them in a 50mL sterile centrifuge tube to obtain Composition for processing biological samples;

[0076] Fully dissolve the above composition in water for injection of corresponding quality to form a colorless and transparent solution;

[0077] In a sterile environment with local 100-level laminar flow purification conditions, the resulting solution is filtered through a 0.22 μm diameter filter membrane into another 50 mL sterile centrifuge tube to obtain a liquid medium for processing biological samples , Keep away from light for later use.

Embodiment 2

[0078] Example 2 Composition of liquid medium

[0079] This embodiment provides a set of liquid media, the composition of which is shown in Table 1. Among them, the liquid media numbered DH001, DH002, DH003, DH004, DH006, DH007, DH008, DH010, DH011 are the liquid media provided by the present invention for processing biological samples, and the liquid media numbered DH005 and DH009 are comparative examples.

[0080] Table 1 Composition of liquid medium (in weight percentage, the balance is water for injection)

[0081]

[0082] Among them, in DH003, HCl represents 1M hydrochloric acid, and 0.0088% represents the mass fraction of solute HCl in hydrochloric acid in the liquid medium.

Embodiment 3

[0083] Example 3 Determination of physical, chemical and biological parameters of liquid medium

[0084] Under the condition of 20°C and humidity less than 60%, the physical, chemical and biological parameters of the liquid medium provided in Example 2 were measured. The test results are shown in Table 2:

[0085] Refer to United States Pharmacopoeia (USP) The described method carries out density determination;

[0086] Refer to the method described in the 2010 edition of the Pharmacopoeia of the People's Republic of China for the determination of osmotic pressure;

[0087] Refer to the method described in the 2010 edition of the Pharmacopoeia of the People's Republic of China for pH determination;

[0088] Refer to the method described in the 2010 edition of the Pharmacopoeia of the People’s Republic of China for sterility determination;

[0089] Refer to the method described in the 2010 edition of the Pharmacopoeia of the People's Republic of China for the determination of bacterial ...

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Abstract

The invention relates to a composition for processing a biological sample. The composition for processing the biological sample is composed of pharmaceutically acceptable hydroxyethyl starch, a pharmaceutically acceptable ionic iodine contrast agent, an osmotic pressure regulator and a pH value modifier according to the mass ratio of (2.0-12.0):(2.5-12.0):(0-0.4):(0-0.5). A part of parameters exceed the traditional commercially available similar products at home and abroad when the composition provided by the invention is applied to gathering a mononuclear cell and the like in the biological sample. Thus, the composition has higher safety and validity, and the possibility is created for clinical translation of the products.

Description

Technical field [0001] The invention relates to a composition and a liquid medium for processing biological samples, and belongs to the technical field of biological materials. Background technique [0002] The use of centrifugation to enrich biological particles in biological samples is the most widely used technology in biological research. Many biological particles can be enriched by centrifugation, such as animal or plant cells, subcellular structures, bacteria, viruses, or biological macromolecules. The performance of these biological samples in suspension under centrifugation mainly depends on the size and density of the biological samples, which can be explained by the Stokes equation. The Stokes equation (see the following equation) describes the changing law of the movement rate of spherical particles when they are subjected to centrifugal action in a liquid medium: [0003] v = d 2 ( p 2 - p 1 ) X g 18 n [0004] Among them...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/078C12N5/0787C12N5/0789C12N5/0775C12N5/0786C12N5/0784
CPCC12N5/0634C12N2500/34C12N2500/60
Inventor 王春有王冬明刘湘连甘朝
Owner BEIJING DONGFANG HUAHUI BIOMEDICAL TECH
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