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Method for carrying out specific capture of circulating tumor cell by using fractal structure surface

A fractal structure and tumor cell technology, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of high cost, unclear basic design principles, inconvenient use, etc., and achieve low cost, simple operation, and improved The effect of capture efficiency

Inactive Publication Date: 2014-03-26
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technology has disadvantages such as high cost and inconvenient use, and the basic design principles behind the technology are not clear

Method used

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  • Method for carrying out specific capture of circulating tumor cell by using fractal structure surface
  • Method for carrying out specific capture of circulating tumor cell by using fractal structure surface
  • Method for carrying out specific capture of circulating tumor cell by using fractal structure surface

Examples

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Effect test

Embodiment 1

[0036] The fractal dimension of the gold surface with a dendritic fractal structure prepared in this example is 2.73; taking circulating tumor cells MCF7 and Jurkat T as examples of circulating tumor cells to be captured, the capture system of the present invention is further described and verified. The method for specific capture of circulating tumor cells using the surface of the fractal structure comprises the following steps:

[0037] (1) A gold surface with a dendritic fractal structure was prepared on the surface of a crystalline silicon substrate by electrochemical deposition

[0038] The traditional three-electrode system is adopted, platinum wire is used as the counter electrode, silver / silver chloride electrode is used as the reference electrode, crystalline silicon substrate is used as the working electrode, and chloroauric acid aqueous solution (1mg / mL) is used as the electrolyte (chloride ion concentration is 0.01 mo / L), a gold surface with a dendritic fractal str...

Embodiment 2

[0054] The fractal dimension of the gold surface with cauliflower-like fractal structure prepared in this example is 2.54; taking circulating tumor cells MCF7 and Daudi as examples of circulating tumor cells to be captured, the capture system of the present invention is further described and verified. The method for specific capture of circulating tumor cells using the surface of the fractal structure comprises the following steps:

[0055] (1) A gold surface with a cauliflower-like fractal structure was prepared on the surface of an indium tin oxide (ITO) glass substrate by electrochemical deposition

[0056] The traditional three-electrode system is adopted, platinum sheet is used as counter electrode, silver / silver chloride electrode is used as reference electrode, indium tin oxide (ITO) glass substrate is used as working electrode, and chloroauric acid aqueous solution (1mg / mL) is used as electrolyte ( Chloride ion concentration is 0.9mo / L), and a gold surface with a cauli...

Embodiment 3

[0072] The fractal dimension of the gold surface with sea urchin-like fractal structure prepared in this example is 2.23; taking circulating tumor cells MCF7 and Jurkat T as examples of circulating tumor cells to be captured, the capture system of the present invention is further described and verified. The method for specific capture of circulating tumor cells using the surface of the fractal structure comprises the following steps:

[0073] (1) A gold surface with a urchin-like fractal structure was prepared on the surface of the gold substrate by electrochemical deposition

[0074] The traditional three-electrode system is adopted, platinum sheet is used as counter electrode, silver / silver chloride electrode is used as reference electrode, gold substrate is used as working electrode, chloroauric acid aqueous solution (1mg / mL) is used as electrolyte (chloride ion concentration is 2mo / L), the preparation of a gold surface with a sea urchin-like fractal structure can be prepa...

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Abstract

The invention relates to the technical field of biology, and particularly relates to a method for carrying out specific capture of a circulating tumor cell by using a fractal structure surface. The method comprises the following steps: preparing the fractal structure surface on the surface of a substrate, then fixing a specific antibody on the surface of a tumor cell on the fractal structure surface and then placing into a cell culture plate; dropwise adding a to-be-tested sample to the surface of the specific antibody at the surface of the tumor cell, and then placing into a cell incubator; specifically capturing the circulating tumor cell in the to-be-tested sample by the synergistic effects of the specific antibody on the surface of the tumor cell and the fractal structure. The capture method based on the fractal structure surface can be applied to capture of cancer-related cells. By adopting the method, the capture efficiency of the circulating tumor cell can be effectively improved, and the method is low in cost, and simple to operate, and can be applied to clinical diagnosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for specifically capturing circulating tumor cells by utilizing the surface of a fractal structure. Background technique [0002] Cancer is the second leading killer of human beings. 90% of these deaths are caused by metastatic cancer. Metastatic cancer is mainly caused by cancer cells detached from the original cancer site, and these cancer cells spread to other tissues or organs of the body through blood vessels or lymphatic vessels, thus forming metastatic cancer. The entry of cancer cells into the blood circulation is the initial stage of cancer metastasis, which provides the possibility for the final formation of metastatic lesions of tumors. This part of the cancer cells entering the peripheral blood of the human body is called circulating tumor cells (Circulating Tumor Cells). By detecting circulating tumor cells in peripheral blood, it can effectively predict the ...

Claims

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Application Information

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IPC IPC(8): C12N5/09
CPCG01N33/574G01N33/54306
Inventor 王树涛张鹏超陈立江雷
Owner INST OF CHEM CHINESE ACAD OF SCI
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