A recessive gene diagnosis chip for Parkinson's disease

A technology of recessive inheritance and disease-causing genes, which is applied in the field of Parkinson's disease recessive genetic disease-causing gene diagnosis chip, can solve the problems of gene detection difficulty, cost, and large manpower and material resources, so as to improve the early diagnosis rate and reduce misdiagnosis The effect of high efficiency and low cost

Active Publication Date: 2015-11-25
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Due to the clinical heterogeneity and polygenic inheritance of Parkinson's disease, genetic testing brings great difficulties and requires considerable manpower and material resources

Method used

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  • A recessive gene diagnosis chip for Parkinson's disease
  • A recessive gene diagnosis chip for Parkinson's disease

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Embodiment

[0015] The gene diagnosis chip for Parkinson's disease of the present invention has a total of 96 mutation point sequences, all of which are AR-PD, wherein parkin 23, PINK1 19, DJ-1 6, ATP13A2 14, PLA2G6 25, FBXO7 9.

[0016] The gene diagnosis chip for Parkinson's disease of the present invention is to immobilize probes capable of hybridizing with mutation points on optical fiber microbeads, and the sequence table of the probes is:

[0017] Numbering sequence 1 ctggcgccgctgcgcgcatgggcctgttcctggcccgcagccgccacctacccagtgaccwtgataggtacgtgggtacctgccaggtacagcctctctgcgccgccccacgccccggtat 2 gcatcttccagctcaaggaggtggttgctaagcgacagggggttccggctgaccagttgcstgtgattttcgcagggaaggagctgaggaatgactggactgtgcaggtgagtctcccttg 3 tgtgacctggatcagcagagcattgttcacattgtgcagagaccgtggagaaaaggtcaakaaatgaatgcaactggaggcgacgaccccagaaacgcggcgggaggctgtgagcgggagc 4 ggaggcgacgaccccagaaacgcggcgggaggctgtgagcgggagccccagagcttgactygggtggacctcagcagctcagtcctcccaggagactctgtg...

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Abstract

A diagnosis chip of recessive pathogenic genetic genes of a Parkinson disease has 96 mutational site sequences, namely 23 Parkin, 19 PINK1, 6 DJ-1, 14 ATP13A2, 25 PLA2G6 and 9 FBXO7. The chip comprises the following test steps of: fabricating an SUD plate, resuspending the SUD plate, fabricating an ASE plate, adding MEL, performing preliminary work on PCR (Polymerase Chain Reaction), combining PCR products, preparing a pre-chip sample, performing chip hybridization, cleaning and scanning the chip, and reading and analyzing data. The diagnosis chip of the recessive pathogenic genetic genes of the Parkinson disease is low in cost, and can screen the recessive pathogenic genetic genes of the Parkinson disease conveniently and rapidly.

Description

technical field [0001] The invention relates to a gene diagnosis chip, in particular to a Parkinson's disease recessive genetic pathogenic gene diagnosis chip. Background technique [0002] Parkinson's disease (parkinson's disease, PD), also known as paralysis agitans (paralysis agitans), is a common neurodegenerative disease. The formation of Lewy bodies (LB) in vivo is a characteristic. It is generally believed that Parkinson's disease is related to multiple factors such as genetics, environment, and aging. With the development of molecular biology, more and more attention has been paid to the role of genetic factors in the pathogenesis of Parkinson's disease. At least 18 pathogenic loci of Parkinson's disease have been located and 13 pathogenic genes have been cloned. From the perspective of inheritance, PD can be autosomal dominant (autosomal dominant prkinson's disease, AD-PD), autosomal recessive (autosomal recessive parkinson's disease, AR-PD), and some cases are s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B40/06
CPCC12Q1/6883C12Q2600/156
Inventor 郭纪锋唐北沙严新翔娄明星
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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