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Method for identifying fermentation suitability degree of tobacco based on UV spectrums

A technology of ultraviolet spectrum and fermentation degree, applied in the field of spectral analysis, to achieve the effect of simple operation method, easy promotion and simple analysis method

Active Publication Date: 2014-03-26
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the above-mentioned studies on the degree of fermentation of tobacco leaves, only a certain type of chemical composition or a certain index in the fermentation process of tobacco leaves was analyzed to evaluate the relationship between the degree of fermentation of tobacco leaves and the quality of tobacco leaves. Evaluate the relationship between the degree of fermentation of tobacco leaves and the quality of tobacco leaves; the iodine value method can only evaluate the relationship between the degree of fermentation of tobacco leaves and the quality of tobacco leaves through the amount of reducing substances (partial chemical components) in the tobacco leaves. These methods are used to judge the degree of fermentation of tobacco leaves. There are certain limitations

Method used

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  • Method for identifying fermentation suitability degree of tobacco based on UV spectrums
  • Method for identifying fermentation suitability degree of tobacco based on UV spectrums
  • Method for identifying fermentation suitability degree of tobacco based on UV spectrums

Examples

Experimental program
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Embodiment 1

[0044] Example 1 Establishment of Moderately Fermented Tobacco Leaf UV Spectrum Library

[0045] Moderately fermented tobacco leaves are the tobacco leaves with the highest sensory evaluation scores during the natural aging process. The tobacco leaf samples and sensory evaluation results for the establishment of spectral library are shown in Table 1 (10 points are used for sensory evaluation).

[0046] It can be seen from Table 1 that Guizhou X2F aged for 13 months, Guizhou C3F aged for 23 months, Hunan B2F aged for 26 months, and Wenshan B2F aged for 25 months had the highest sensory evaluation scores, so Guizhou X2F and Guizhou aged for 13 months were selected Guizhou C3F aged for 23 months, Hunan B2F aged for 26 months, and Wenshan B2F aged for 25 months were used as standard samples for establishing the UV spectrum library of moderately fermented tobacco leaves. The tobacco leaves used in this embodiment are selected from the tobacco leaf samples being used in tobacco fac...

Embodiment 2

[0051] Example 2 Discrimination of X2F Fermentation Suitability in Guizhou

[0052] Preparation of the test solution: Accurately weigh 0.1g of Guizhou X2F tobacco leaves dried to constant weight, reflux with 25ml petroleum ether, 25ml chloroform, 50ml absolute ethanol, and 50ml water for 1 hour, and filter to obtain petroleum ether extract and chloroform extract , water extract, and absolute ethanol extract, the petroleum ether extract, chloroform extract, and water extract were diluted 2 times with corresponding solvents respectively, and the absolute ethanol extract was diluted 20 times with absolute ethanol.

[0053] Ultraviolet spectrum collection and processing: Put the test solution in a 1cm quartz cuvette, use the corresponding solvent as a blank control, use an ultraviolet spectrophotometer to scan in the wavelength range of 190nm to 400nm, and record the ultraviolet spectra of different solvent parts.

[0054] Discrimination of fermentation suitability: the ultraviole...

Embodiment 3

[0070] Example 3 Discrimination of C3F Fermentation Suitability in Guizhou

[0071] Preparation of the test solution: Accurately weigh 0.1g of Guizhou C3F tobacco leaves dried to constant weight, reflux with 25ml petroleum ether, 25ml chloroform, 50ml absolute ethanol, and 50ml water for 1 hour, and filter to obtain petroleum ether extract and chloroform extract , water extract, and absolute ethanol extract, the petroleum ether extract, chloroform extract, and water extract were diluted 2 times with corresponding solvents respectively, and the absolute ethanol extract was diluted 20 times with absolute ethanol.

[0072] Ultraviolet spectrum collection and processing: Put the test solution in a 1cm quartz cuvette, use the corresponding solvent as a blank control, use an ultraviolet spectrophotometer to scan in the wavelength range of 190nm to 400nm, and record the ultraviolet spectra of different solvent parts.

[0073] Discrimination of fermentation suitability: the ultraviole...

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Abstract

The invention belongs to the technical field of spectrum analysis, and discloses a method for identifying fermentation suitability degree of tobacco based on UV spectrums. The method comprises the following steps: preparing moderately-fermented tobacco samples from different regions and at different grades, and a test liquid of the tobacco samples to be tested; acquiring the UV spectrums of the tobacco samples to establish a UV spectrum graph library for the moderately-fermented tobacco; acquiring the UV spectrums of tobacco samples to be tested, comparing the UV spectrums of the tobacco samples to be tested with the UV spectrums, of corresponding tobacco from the same production region and at the same grade, in the UV spectrum graph library; judging the fermentation suitability degree of the tobacco to be tested. According to the invention, four different solvents of petroleum ether, chloroform, absolute ethyl alcohol, and water are adopted to extract tobacco components with different polarities; the fermentation degree of the tobacco can be judged accurately in an overall and macroscopic manner according to UV spectrum differences caused by differences in fermentation degree of the tobacco; the identifying method for quantitatively judging the fermentation suitability degree of the tobacco is adopted, and scientific validation is made; the method is stable, scientific, and feasible.

Description

technical field [0001] The invention belongs to the technical field of spectral analysis, and relates to a method for identifying the suitability of fermentation of tobacco leaves, and more specifically, to a method for identifying the suitability of fermentation of tobacco leaves based on ultraviolet spectrum. Background technique [0002] The cigarette industry refers to the dry tobacco leaves that have been threshed, rebaked and unfermented as new tobacco. New cigarettes are not suitable for smoking, let alone be directly used for industrial production of cigarettes, and must be fermented. During this process, the green miscellaneous gas and irritation of the tobacco leaves will be greatly reduced, the aroma will be revealed, the taste will be mellow, and the color will be even and deepened. However, as the storage time continues to prolong, the quality of the tobacco leaves will develop in the direction of deterioration, the color of the tobacco leaves is too dark, the ...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 李旭华杨庆肖明礼陈越立包秀萍李仙
Owner CHINA TOBACCO GUANGDONG IND
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