Recombinant protein for detecting hog cholera virus, preparation method and application thereof

Abstract

The invention discloses a recombinant protein for detecting a hog cholera virus. The recombinant protein is encoded by a fusion gene which is formed by splicing a surface antigen single-chain antibody gene of anti-human red blood cell H and an envelop glycoprotein single-chain antibody gene of anti-hog cholera virus E2. The recombinant protein has a double-function characteristic, can be conjugated with the human red blood cell without agglutination and further conjugated with the hog cholera virus, and under the virus binding action, generates agglutination megascopically. The recombinant protein can be used for detecting the hog cholera virus, is simple in operation, high in flexibility and strong in specificity, and can meet the requirement of quickness, simpleness and convenience for use in the grass-root level field.

Description

technical field [0001] The invention belongs to the technical field of recombinant proteins, and in particular relates to a recombinant protein for detection of swine fever virus and its preparation method and application. Background technique [0002] Classical Swine Fever (CSF) is a highly contagious infectious disease caused by swine fever virus (CSFV) that seriously endangers the global pig industry. The World Organization for Animal Health (OIE) lists it as a Class A infectious disease One of the major animal diseases planned for eradication. Animal diseases such as swine fever have always been an important factor hindering the healthy development of my country's pig industry, causing direct economic losses of tens of billions of yuan each year, and the economic losses caused by the existence of swine fever affecting the export of live pigs and their products are even more immeasurable. [0003] Timely and accurate detection of CSFV is the prerequisite for diagnosis, p...

AI Technical Summary

Problems solved by technology

Current CSFV detection techniques include susceptible animal challenge test, rabbit interactive immune test, ELISA antigen capture method, immunofluorescence test, reverse transcription-polymerase chain reaction, real-time fluorescent quantitative reverse transcription-polymerase chain reaction, nucleic acid Probe technology and gene chip technology, etc., but these detection methods have high requirements, need to be equipped with special instruments and professional technicians, the detection cost is expensive, and it takes a relatively long time, which greatly limits its application in grassroots units

Our country is still dominated by small and medium-scale pig farms in rural areas. Due to the limitations of economic and technical conditions, these pig farms cannot establish their own testing laboratories. Therefore, it is urgent to establish a fast, simple, low-cost, no special equipment, suitable CSFV detection reagents and methods for grassroots and field use

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