Preparation method of fish collagen double sustained-release film
A double sustained-release, fish collagen technology is applied in the directions of pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., to achieve the effects of simple and convenient preparation process, reduced toxic and side effects, and mild conditions.
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Embodiment 1
[0021] Example 1: A preparation method of fish collagen-based double sustained-release drug film
[0022] 1) Weigh 130 mg of GC and dissolve in 6 mL of distilled water, and dissolve 72.8 mg of CHS in 20 mL of 65% ethanol solution, then add 38.0 mg of EDC and 22.9 mg of NHS, stir for 10 h, then slowly add to the GC solution, at 40°C After stirring and reacting for 24 h, dialyze with 80% ethanol solution and distilled water for 24 h respectively, and freeze-dry to obtain CHGC; weigh 0.25 g of CHGC, 0.37 g of biotin, 0.20 g of EDC, and 0.12 g of NHS and dissolve them in 30 mL of DMSO The solution was reacted at 20°C for 24 h, dialyzed in distilled water for 48 h, and freeze-dried to obtain bio-CHGC.
[0023] 2) Dissolve 0.12 g bio-CHGC in 20% acetic acid solution, sonicate the probe for 2 min under ice bath conditions, ultrasonic power is 80 W, sonicate 5 times, and dialyze in distilled water for 24 h to obtain bio-CHGC self-aggregated nanoparticles grain. 800 μL of 5 mg / mL dox...
Embodiment 2
[0026] Example 2: A preparation method of fish collagen-based double sustained-release drug film
[0027] 1) Weigh 130 mg of GC and dissolve in 8 mL of distilled water, and dissolve 72.8 mg of CHS in 20 mL of 75% ethanol solution, then add 38.0 mg of EDC and 22.9 mg of NHS, stir for 10 h, then slowly add to the GC solution, at 40°C After stirring and reacting for 72 h, dialyze with 80% ethanol solution and distilled water for 24 h respectively, and freeze-dry to obtain CHGC; weigh 0.25 g of CHGC, 0.37 g of biotin, 0.20 g of EDC, and 0.12 g of NHS and dissolve them in 40 mL of DMSO The solution was reacted at 40°C for 24 h, dialyzed in distilled water for 48 h, and freeze-dried to obtain bio-CHGC.
[0028] 2) Dissolve 0.12g bio-CHGC in 20% acetic acid solution, sonicate the probe for 2 min under ice bath conditions, ultrasonic power is 80 W, sonicate 5 times, and dialyze in distilled water for 24 h to obtain bio-CHGC self-aggregated nanoparticles grain. Slowly add 1.6 mL of 5...
Embodiment 3
[0031] Example 3: In vitro release experiment of fish collagen-based double sustained-release drug film
[0032] Weigh a certain amount of fish collagen-based double slow-release drug film or drug-loaded nanoparticles into the dialysis bag, then completely submerge the sealed dialysis bag in 10 mL of PBS solution (pH 7.4), at 37°C, 110 rpm Shake in the dark under the conditions, take out all the release medium on 1, 3, and 5 days, and pour into an equal volume of fresh medium, measure the release amount of the drug at 480 nm with a UV-visible spectrophotometer and calculate the cumulative Release rate (Q%). Its formula is as follows:
[0033]
[0034] C n : T n The concentration of the sample at the time point, μg / mL; V: the total volume of the release medium, mL; V i : T i The volume of medium released at the time point, mL; C i : T i Concentration of samples at time points, μg / mL; W DBCN : mass of drug-loaded nanoparticles, μg; LC%: drug-loaded amount of drug-load...
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