Method and device for high temperature sterilization by using steam explosion

A blasting equipment and high-temperature sterilization technology, applied in heating and other directions, can solve the problems of easy agglomeration and long time of the culture medium, and achieve the effects of shortening the sterilization cycle, reducing the damage rate, and shortening the high temperature maintenance time.

Inactive Publication Date: 2014-04-16
北京中科百瑞能工程技术有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, conventional steam sterilization takes a long time due to the cooling time, and the medium is easy to agglomerate at high temperature

Method used

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  • Method and device for high temperature sterilization by using steam explosion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The bran soybean meal medium is sterilized by steam explosion high-temperature sterilization equipment, and then solid-state fermentation is used to produce feed additives.

[0020] The steam explosion equipment with a height-to-diameter ratio of 1:0.25, a diameter of 1.2m, and a cylinder height of 4.8m is used. The culture medium is composed of bran and soybean meal, the mass ratio of which is 9:1. After the two are fully mixed, 1.1 times (mass ratio) of inorganic salt solution is added. The composition of the inorganic salt solution is: (NH 4 ) 2 SO 2 ,,10 g / L, KH 2 PO 4 , 20 g / L, MnSO 4 , 0.4 g / L, CaCO 3 40 g / L, pH6.5-7.0.

[0021] Before the medium is sterilized, the equipment and pipelines should be sterilized first. Open the steam inlet valve (2) or (3) and the discharge valve (5), and the high-temperature steam is introduced from the steam generator, which is the steam explosion equipment and the feeding pipeline (6), the receiving tank (9), and the disch...

Embodiment 2

[0024] The steam explosion high temperature sterilization equipment is used to sterilize the steam explosion corn stalk and wheat bran medium, and then solid-state fermentation to produce cellulase.

[0025] The steam explosion equipment with a height-to-diameter ratio of 1:0.25, a diameter of 1.2m, and a cylinder height of 4.8m is used. The culture medium consists of steam-exploded corn stalks and wheat bran, the mass ratio of which is 4:1. After the two are fully mixed, 2.5 times (mass ratio) of inorganic salt solution is added. The composition of the inorganic salt solution is: (NH 4 ) 2 SO 4 , 15 g / L; MgSO 4 , 6 g / L; KH 2 PO 4 , 3 g / L.

[0026] Before the medium is sterilized, the equipment and pipelines should be sterilized first. Open the steam inlet valve (2) or (3) and the discharge valve (5), and the high-temperature steam is introduced from the steam generator, which is the steam explosion equipment and the feeding pipeline (6), the receiving tank (9), and the ...

Embodiment 3

[0029] Steam explosion high-temperature sterilization equipment is used to sterilize steam-exploded wheat straw and wheat bran medium, and then solid-state fermentation to produce cellulase.

[0030] The steam explosion equipment with a height-to-diameter ratio of 1:0.25, a diameter of 1.2m, and a cylinder height of 4.8m is used. The culture medium consists of steam-exploded wheat straw and wheat bran with a mass ratio of 4:1. After the two are fully mixed, 2.5 times (mass ratio) of inorganic salt solution is added. The composition of the inorganic salt solution is: (NH 4 ) 2 SO 4 , 15 g / L; MgSO 4 , 6 g / L; KH 2 PO 4 , 3 g / L.

[0031] Before the medium is sterilized, the equipment and pipelines should be sterilized first. Open the steam inlet valve (2) or (3) and the discharge valve (5), and the high-temperature steam is introduced from the steam generator, which is the steam explosion equipment and the feeding pipeline (6), the receiving tank (9), and the discharge port ...

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Abstract

The invention provides a method and device for high temperature sterilization by using steam explosion, which are short in sterilization cycle, low in destruction rate of nutrient substances, loose in materials, easy to amplify, and high in production efficiency aiming at the defects that conventional high-pressure steam sterilization is long in cycle, serious in damage to nutrient substances, easy in caking and the like. According to the method and device provided by the invention, the sterilization time is shortened by improving the sterilization temperature, so as to possibly reduce the damage to the nutrient substances. Meanwhile, the materials and the steam are ejected in a burst state in a rapid emptying manner, so as to loosen a culture medium and simultaneously shorten the cooling time. Thus, the overall sterilization cycle is shortened. The method and the device can facilitate industrial production of solid state fermentation by improving the production efficiency and the fermentation effect of the culture medium.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method and equipment for high-temperature sterilization using steam explosion. Background technique [0002] Since the fermentation industry adopts purebred culture, the yield and quality of the product have been greatly improved, and the requirements for preventing contamination are also higher. At present, various culture processes are often required to be carried out without contamination by bacteria. Since the culture process usually contains relatively rich nutrients, and there are often various microorganisms in the culture medium, it is easy to be polluted by bacteria. This will lead to various adverse consequences. [0003] It can be seen that whether the medium is completely sterilized is directly related to the success or failure of the production process. In the slightest, it will lead to a sharp decrease in the output of the required products, a decl...

Claims

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Application Information

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IPC IPC(8): A61L2/07
Inventor 魏远
Owner 北京中科百瑞能工程技术有限责任公司
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