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Process for removing newborn bovine serum endotoxin

A newborn bovine serum and endotoxin technology, applied to embryonic cells, vertebrate cells, animal cells, etc., can solve problems such as bacterial contamination, increase efficiency and added value, reduce endotoxin content, and lower serum protein concentration Effect

Active Publication Date: 2014-04-16
JINAN JINNIU BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

[0003] In the existing production process of newborn bovine serum, due to bacterial contamination caused by many reasons such as blood collection and storage, a large amount of serum with excessive endotoxin will be produced. Therefore, in order to make full use of biological resources and reduce resource waste, a newborn bovine serum is designed The process of endotoxin is a problem that needs to be solved urgently

Method used

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  • Process for removing newborn bovine serum endotoxin
  • Process for removing newborn bovine serum endotoxin
  • Process for removing newborn bovine serum endotoxin

Examples

Experimental program
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Effect test

Embodiment

[0009] Embodiment Removes newborn bovine serum endotoxin experiment

[0010] (1) Preparation of raw material newborn bovine serum

[0011] The newborn bovine serum with higher endotoxin content in the production process of the newborn bovine serum is used as the raw material bovine serum of the present invention.

[0012] The serum endotoxin content was detected using the Limulus Reagent Endotoxin Detection Kit from Zhanjiang Bokang Marine Biological Co., Ltd.

[0013] (2) Process for removing endotoxin from bovine serum

[0014] Slowly sprinkle the weighed different dosages of activated carbon (0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 1%, 2%, 5%) into the serum and gently stir until uniform. After continuous gentle stirring for 30 minutes, Activated carbon was removed by filtration step by step to obtain newborn bovine serum. The filtered serum has been verified by the sterility test and meets the sterility requirements.

[0015] (3) Detection of endotoxin content

[0016] Dissolve...

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Abstract

The invention discloses a process for removing newborn bovine serum endotoxin. The process comprises the following steps: taking newborn bovine serum, centrifuging for 10 minutes at a speed of 4000 revolutions per minute, taking the supernatant, adding activated carbon which accounts for 0.2 percent of the mass of the supernatant for adsorbing, wherein the adsorption time is 30 minutes; centrifuging for 20 minutes at a speed of 4000 revolutions per minute after adsorption, taking the supernatant, sequentially filtering by using filter membranes with the size of 0.8mu m, 0.65mu m, 0.45mu m, 0.22mu m and 0.1mu m so as to obtain the newborn bovine serum in which the endotoxin is removed. By adoption of the activated carbon adsorption and step-by-step filtering method, the content of the endotoxin in the high-endotoxin-content bovine serum serving as wastes can be obviously reduced in the previous newborn bovine serum production process, and the production efficiency and additional value of the newborn bovine serum are improved.

Description

technical field [0001] The invention relates to a process for removing serum endotoxin of newborn bovine. Background technique [0002] Endotoxin is a general term for toxic substances present in the cells of Gram-negative bacteria. It is a component of the cell walls of various Gram-negative bacteria. Its chemical composition is phospholipopolysaccharide-protein complex, and its toxic composition is mainly lipid A. Endotoxins are located in the outermost layer of the cell wall, on mucinous peptides that coat the cell wall. Most of the endotoxins of various bacteria have certain toxicity, which can cause fever, microcirculation disturbance, endotoxin shock and disseminated intravascular coagulation. Therefore, serum containing endotoxin cannot be used for vaccine production. Only by removing endotoxin and retaining the nutrients in serum can this type of serum be used in vaccine production, thereby turning waste into treasure and fully utilized. [0003] In the existing p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 张子毅
Owner JINAN JINNIU BIOTECH CO LTD
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