Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling

A technology of subculture medium and tissue culture seedlings, which is applied in the field of basic medium for the subculture of saline-alkali-tolerant fast-growing Ulmus elm tissue culture seedlings, can solve the problems of low value-added rate of medium, high cost, resource waste, etc., and achieve Increased average high multiplication rate, vigorous growth, and reduced production costs

Inactive Publication Date: 2014-04-23
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The value-added rate of the above-mentioned culture medium is low, which causes high cost and waste of resources in the factory production of Baiyu

Method used

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  • Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling
  • Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling
  • Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling

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Embodiment 1

[0017] Pretreatment of the tissue-cultured seedlings of Ulmus ulmis: select the tissue-cultured seedlings of Ulmus ulmis with the same growth cycle, culture conditions, and health, and cut the tissue-cultured seedlings of Ulmus ulmis into multiple single buds and clustered buds of the same size for later use.

[0018] The growth environment required for the tissue cultured seedlings of Ulmus ulmiformis is: temperature 25°C (± 2°C), 12 hours of light time, subculture and culture for 21 days in a sterile environment every day.

[0019] The invention mainly uses basic medium for comparison and screening, adds low-concentration proliferative hormone 6-BA to ensure the normal growth of Ulmus elm tissue culture seedlings, and agar 7g / L and white sugar 30g / L as auxiliary materials.

[0020] In order to compare the difference between the existing white elm production medium and the present invention, the present invention selects ms+BA0.2+S3% (A1), EM+6-BA0.2+S3% (A2), dkw+6-BA0. 2+S3...

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Abstract

The invention discloses a subculture medium for a saline / alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling. The subculture medium comprises the following components: CaCl2.2H2O, NH4NO3, KNO3, NaCl, MgSO4, NaH2PO4.2H2O, K2SO4, Na2SO4, CaCl2, CoCl2.6H2O, CuSO4.5H2O, H3BO3, MnSO4.H2O, Na2MoO4.2H2O, ZnSO4.7H2O, KI, FeSO4.7H2O, Na2EDTA.2H2O, inositol, glycine, nicotinic acid, V6, V1, biotin, folic acid, riboflavin and vitamin. Compared with a traditional culture medium, the subculture medium disclosed by the invention improves the average reproduction rate of the ulmus pumila L. tissue culture seedling by more than 5 times, and improves the average reproduction coefficient by more than 2.3 times.

Description

technical field [0001] The invention relates to a basic culture medium for tissue-cultured seedlings, in particular to a series of basic culture media for the subculture of saline-alkali-tolerant fast-growing Ulmus ulmis tissue-cultured seedlings. Background technique [0002] White elm (Ulmus pumila L.), a plant of the genus Ulmus in the family Ulmaceae, has many varieties, and elm has always been the top five native garden tree species in northern my country [Populus, Salix, elm (Ulmus), Chinese pagoda tree (Sophora), Ailanthus (Ailanthus)], and one of the world's four major street greening tree species, with a wide range of applications. [0003] White elm is a native tree species with a long history of cultivation in my country. It is mostly used for afforestation in saline-alkali land and arid and barren areas because of its characteristics of drought resistance, high temperature resistance, severe cold resistance, fast growth, good material, resistance to barrenness, an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 慕徳宇房用李周岐慕宗昭梁玉王月海王强
Owner NORTHWEST A & F UNIV
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