Preparation method of microbial compound fertilizer

A compound fertilizer and microbial technology, applied in the field of preparation of microbial compound fertilizer, can solve the problems of waste, high cost, insufficient fertilization, etc., and achieve the effect of increasing vegetable yield

Inactive Publication Date: 2014-04-23
石家庄先农达生物化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its shortcoming is: using above-mentioned three kinds of bio-fertilizers alone can only solve one aspect of the nutrition needed by plants, and the cost is high, and the dosage is large. deficiency or waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] (1) Preparation of bacterial suspension

[0019] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.

[0020] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0021] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...

Embodiment 1

[0057] A kind of microbial compound fertilizer, is made up of Aspergillus niger culture and Bacillus subtilis inoculum, the weight part array of microbial compound fertilizer is 40 parts of Aspergillus niger culture, 20 parts of Bacillus subtilis inoculum;

[0058] The concrete production method step of product of the present invention is as follows:

[0059] Bacteria culture preparation:

[0060] Preparation of Bacillus subtilis agent culture: transfer and cultivate Bacillus subtilis from the slope, transfer the seed liquid after step-by-step expansion into the fermenter, and centrifuge after fermentation to obtain wet bacteria and fermentation liquid; after fermentation, the fermentation liquid is subjected to low-temperature negative Concentrate under vacuum to 50% of the original volume to obtain a bacterial concentrate. Add carrier: Add the mixed carrier to the concentrated solution and mix evenly; the weight ratio of the concentrated solution to the carrier is 0.6:1, an...

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PUM

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Abstract

The invention discloses a preparation method of a microbial compound fertilizer, and particularly relates to a fertilizer in agricultural technique. The preparation method of the microbial compound fertilizer comprises the following steps: respectively culturing and fermenting selectd Aspergillus niger nutrient material and Bacillus subtilis, and proportionally combining the fermentation products to obtain the microbial compound fertilizer. The microbial compound fertilizer is prepared from the following components in parts by weight: 20-50 parts of Aspergillus niger nutrient material and 20-35 parts of Bacillus subtilis microbial inoculum. The experiment proves that the product can enhance the grain yield by 8-30% and enhance the vegetable yield by 10-40%, and can also improve the quality and flavor of the vegetables and grains. The consumption of the compound fertilizer for each mou of land is 50-100g, and thus, the microbial compound fertilizer is one of low-consumption microbial compound fertilizers at home and abroad at present.

Description

Technical field: [0001] The invention relates to fertilizers in agricultural technology, and relates to a preparation method of a novel microbial compound fertilizer. Background technique: [0002] Most of the existing biological fertilizers are single biological fertilizers. Such as: nitrogen-fixing bacterial fertilizer, which is mainly composed of bacteria and fungi with nitrogen-fixing effect; another example: phosphorus bacteria, whose function is to decompose organic phosphorus in the soil, making it easy to be absorbed by plants; another example: bacteria, Its function is to decompose potassium compounds in the soil and make them into available potassium that can be absorbed and utilized by plants. These fertilizers can play their own roles when used alone, and partially meet the needs of plants for the three elements of nitrogen, phosphorus, and potassium. They do not produce the soil compaction phenomenon caused by long-term use of chemical fertilizers. But its sh...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 邵素英孔日祥
Owner 石家庄先农达生物化工有限公司
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