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Method for extraction and composition analysis of peony stamen grease

A technology of peony and stamens, which is applied in the field of extraction and component analysis of plant oils, oil extraction and component analysis in peony stamens, and can solve the problems of oil component analysis and pharmacological effects in peony stamens that have not been reported in literature and patent reports. Achieve high economic and social benefits, less solvent consumption, and good environmental benefits

Inactive Publication Date: 2014-04-23
QINGDAO AGRI UNIV
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Problems solved by technology

[0005] At present, there are no literature and patent reports on the extraction, component analysis and pharmacological effects of the oil in peony stamens at home and abroad.

Method used

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  • Method for extraction and composition analysis of peony stamen grease

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Embodiment 1

[0016] 1) Extraction of oil from peony stamens: select the peony variety Fengdan, collect unloose powdered peony stamens, and dry at 45°C until constant weight. Crush the dried stamens and pass through a 20-100 mesh sieve for later use. Weigh 3 g of sample, wrap it tightly with filter paper and put it into a Soxhlet extractor, add 150 mL of ether solution, heat in a water bath for 10 hours, and keep the temperature at 45-55 °C. After the extraction is completed, the extract is distilled off under reduced pressure to remove the solvent to obtain the peony stamen oil. Weigh the quality of the oil in the stamens of peony, and the calculation shows that the content of oil in the stamens of peony reaches more than 8%.

[0017] 2) Methylation of peony stamen oil: take 1 mL of peony stamen oil, add 10 mL of methanol and 1 mL of concentrated sulfuric acid solution, stir and heat under reflux for 10 min until the solution is clear and transparent. The components after the methyl este...

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Abstract

The invention relates to a method for extraction and composition analysis of peony stamen grease. The method comprises the steps: collecting peony stamens, drying, then crushing, placing into a Soxhlet extractor, adding a proper mount of a small-polar solvent in a round-bottom flask at the bottom of the soxhlet extractor, and carrying out water-bath heating for repeated extraction; after finishing extraction, carrying out reduced pressure distillation of the extracted liquid to remove the solvent, and thus obtaining the peony stamen grease; carrying out methyl esterification of the obtained peony stamen grease, and analyzing chemical components and composition by GC-MS. Analysis indicates that the main components of the peony stamen grease comprise linolenic acid, palmitic acid, 8,11-octadecadienoic acid and multiple unsaturated fatty acids, and the nutrient components have extremely important value to human body health; and the peony stamen grease can be widely applied in the fields of foodstuffs, health products, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of vegetable oil extraction and component analysis, in particular to the technical field of oil extraction and component analysis in peony stamens. Background technique [0002] Peony (scientific name: Paeonia suffruticosa Andr.): Angiosperms, perennial deciduous shrubs, flower colors are red, pink, yellow, white, green, purple, etc.; stamens are mostly stamens. [0003] Domestic peony production is mainly concentrated in Heze and Luoyang. The planting area of ​​peonies in Heze has exceeded 100,000 mu, with more than 1,000 varieties. [0004] Vegetable oils are closely related to human life, among which the health care and medicinal value is a very important aspect. Among the fatty acids in vegetable oils, unsaturated fatty acids (such as oleic acid, linoleic acid, and linolenic acid) are necessary for the human body and play an important role in reducing blood cholesterol levels, preventing cardiovascul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04C11C3/04G01N30/08
Inventor 郑国生史美丽周远明马传利于曙光
Owner QINGDAO AGRI UNIV
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