Method for synthesizing pyrroloquinoline quinone through lactobacillus fermentation
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A technology for pyrroloquinoline quinone and lactic acid bacteria fermentation, applied in the field of microbial fermentation
Active Publication Date: 2014-04-23
ZHEJIANG UNIV
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Problems solved by technology
Unfortunately, none of the currently discovered or recombinantly constructed bacteria with the ability to synthesize PQQ are food-grade safe microorganisms. Therefore,
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Embodiment 1
[0057] Such as figure 1 Shown, the present invention synthesizes the method for pyrroloquinoline quinone by lactic acid bacteria fermentation, comprises the following steps:
[0058] (1) Lactobacillus plantarum PQQ-1 was purchased from Jiaxing Yuanke Biotechnology Co., Ltd. with the product number YK-S-003.
[0059] (2) Each culture medium was prepared according to the following components, among which, the yeast extract and peptone were both Sigma products, and the barley flour was a product of Jinmaiyuan Mairenchang, Dafeng City, Jiangsu Province; other reagents were of analytical grade; After completion, all were sterilized at 121°C for 15 minutes; after preparation, all were sterilized at 121°C for 15 minutes;
[0060] (a): 1L of MRS solid plate culture medium includes the following components by weight:
[0061]
[0062]
[0063] Adjust the pH to 6.5±0.2 with sulfuric acid.
[0064] (b): Agar subculture medium (A) is calculated in 1L, including the following comp...
Embodiment 2
[0090] Such as figure 1 Shown, the present invention synthesizes the method for pyrroloquinoline quinone by lactic acid bacteria fermentation, comprises the following steps:
[0091] (1) Each culture medium was prepared according to the following components. After the preparation was completed, they were all sterilized at 121°C for 15 minutes. The yeast extract and peptone were all Sigma products, and the barley flour was Jinmaiyuan wheat kernels from Dafeng City, Jiangsu Province. Chang products; other reagents are of analytical grade; after the preparation is completed, all are sterilized at 121°C for 15 minutes;
[0092] (a): Agar subculture medium (A) is calculated in 1L, including the following components by weight:
[0093]
[0094] And the agar subculture medium was adjusted to pH 6.8 with acid-base buffer.
[0095] (b): Activation medium (B) is calculated in 1L, including the following components by weight:
[0096]
[0097] And the activated medium was adjust...
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Abstract
The invention discloses a method for synthesizing pyrroloquinoline quinone through lactobacillus fermentation. The method comprises the following steps: activating the lactobacillus plantarum PQQ-1 which is stored in an agar subculture medium through an activation culture medium, and then carrying out stationary culture of the lactobacillus plantarum PQQ-1 at a temperature ranging from 20 to 40 DEG C for 16-30h to from primary seeds; further carrying out enlarging stationary culture of the primary seeds at a temperature ranging from 20 to 40 DEG C through a primary seed culture medium for 16-30h to form secondary seeds; and further carrying out enlarging stationary culture of the secondary seeds at a temperature ranging from 20 to 40 DEG C through a secondary seed culture medium for 42-54h and then starting the fermentation stage, and carrying out anaerobic culture in a production fermentation culture medium at a temperature ranging from 20 to 40 DEG C for 54-66h to obtain the PQQ. The used lactobacillus is a food-grade safe microorganism; and the PQQ is obtained through fermentation, and is high in yield and bright in application prospect.
Description
technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for synthesizing pyrroloquinoline quinone by fermentation of lactic acid bacteria. Background technique [0002] Pyrroloquinoline quinone (PQQ for short) is the third prosthetic group found in bacterial dehydrogenase after flavin nucleotide and nicotinamide nucleotide, and its chemical name is 4,5-dihydro -4,5-dioxide-1-hydropyrrole (2,3f)quinone-2,7,9-tricarboxylic acid, also known as Methaxatin. Under natural conditions, only a few bacteria such as Methylovorus, Deinococcus radiodurans, Gluconobacter oxydans, and Klebsiella pneumoniae can synthesize it (Xiong et al, 2011; Magnusson et al, 2007; Khairnar et al, 2003; Felder et al, 2000). [0003] Although only a few bacteria can synthesize PQQ, trace amounts of PQQ are found in various plants, animals and humans. Japanese scientists determined the PQQ content in 26 common foods and found that the PQQ content in 1...
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