Normal temperature preserving liquid for equine animal embryo, and preparation method and application thereof

A technology for preservation and embryos at room temperature, applied in the field of animal embryo transfer, can solve the problems of high price, difficult drug sources, complicated preparation steps, etc., and achieves the effects of convenient material collection, promotion and application, and simple preparation.

Inactive Publication Date: 2014-04-30
QINGDAO DERUI JUNFA BIOLOGY TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of difficult sources of medicines for traditional embryo room temperature preservation solutions, high prices, cumbersome preparation steps, and the need for immediate use and p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A solution for storing equine animal embryos at room temperature includes 5000ml of sodium lactate mixture as the main solution, and each 1000ml of sodium lactate mixture contains 500,000 units of penicillin, 500,000 units of streptomycin and 10% by volume of calf serum.

[0022] Weigh 27g of sodium lactate, 16g of sodium chloride, 23g of potassium chloride, 5.5g of calcium chloride and 1.6g of magnesium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 6 hours, and set aside; weigh 0.4g of astragalus polysaccharide Put it into a sterile beaker, add it to the spare mixed solution into the sterile beaker, stir to fully dissolve the astragalus polysaccharide, and obtain a sodium lactate mixed solution; before storing the embryos at room temperature, add penicillin, Streptomycin and calf serum can be used to obtain embryo room temperature preservation solution.

[0023] Use the embryo room temperature preservation solution of this embodiment...

Embodiment 2

[0025] A normal temperature preservation solution for equine animal embryos, including 5000ml of sodium lactate mixed solution as the main solution, and every 1000ml of sodium lactate Ringer injection contains 1 million units of penicillin, 1.2 million units of streptomycin and 3% inactivated horse serum by volume.

[0026] Weigh 10.5g of sodium lactate, 6g of sodium chloride, 13.5g of potassium chloride and 9g of calcium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 3 hours, and set aside; weigh 1.4g of astragalus polysaccharide into Add it to the sterile beaker, add it to the spare mixed solution, stir to fully dissolve the astragalus polysaccharide, and get the sodium lactate mixed solution; before storing the embryos at room temperature, add penicillin and streptomycin to the sodium lactate mixed solution according to the component content. And inactivated horse serum, the embryo room temperature preservation solution can be obtained.

...

Embodiment 3

[0029] A normal temperature preservation solution for equine animal embryos, including 4000ml of sodium lactate mixture as the main solution, wherein every 1000ml of sodium lactate mixture contains 800,000 units of penicillin, 800,000 units of streptomycin and 5% inactivated horse serum by volume; Wherein, the content of each component in the sodium lactate mixed solution is: every 1000ml sodium lactate mixed solution contains 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride.

[0030] Weigh 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride, add 4000ml of distilled water to mix, and place in a constant temperature box at 37°C for 1 hour to obtain a sodium lactate mixture for use; store the embryos at room temperature Before, add penicillin, streptomycin and inactivated horse serum to the sodium lactate mixture according to the component content to obtain embryo room tem...

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PUM

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Abstract

The invention discloses a normal temperature preserving liquid for equine animal embryos, and a preparation method and application thereof. The normal temperature preserving liquid includes a sodium lactate mixed solution, penicillin, streptomycin, and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum. The preparation method includes the steps of: placing the sodium lactate mixed solution in a thermostat at 37 DEG C for 0.5-6 h for standby; and adding penicillin, streptomycin and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum into the sodium lactate mixed solution in accordance with the component contents, so as to obtain the normal temperature preserving liquid for embryos. The preserving liquid is suitable for non-surgical method for embryo transplantation of horses, cattle and donkeys. The invention has the beneficial effects of convenience for material acquisition, low price, simple preparation and convenience for usage, is very suitable for scale production of non-surgical method for embryo transplantation of equine animals, and is favorable for popularization and application of embryo transplantation technology.

Description

Technical field [0001] The present invention is the field of animal embryo transplantation, especially involving a horseman animal room temperature preservation solution and its preparation methods and applications. Background technique [0002] Embryo transplantation, also known as fertilized egg transplantation, commonly known as artificial tires or abdominal pregnant, refers to the transplantation of female animals into other female animals with the same type of physiological state, which continues to develop into a new individual technology.Basic procedures for embryo transplantation include superpatients and fertilization of supply, estrus treatment, embryo, and transplantation during the same period of receptor. [0003] The embryo room temperature preservation liquid is a protective nutritional liquid for embryo when the embryo room temperature is preserved.At present, there are rarely reports on the cooled temperature preservation of embryos at home and abroad.Especially ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 荣美洁孙腾飞邢金凤
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
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