Normal temperature preserving liquid for equine animal embryo, and preparation method and application thereof
A technology for preservation and embryos at room temperature, applied in the field of animal embryo transfer, can solve the problems of high price, difficult drug sources, complicated preparation steps, etc., and achieves the effects of convenient material collection, promotion and application, and simple preparation.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] A solution for storing equine animal embryos at room temperature includes 5000ml of sodium lactate mixture as the main solution, and each 1000ml of sodium lactate mixture contains 500,000 units of penicillin, 500,000 units of streptomycin and 10% by volume of calf serum.
[0022] Weigh 27g of sodium lactate, 16g of sodium chloride, 23g of potassium chloride, 5.5g of calcium chloride and 1.6g of magnesium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 6 hours, and set aside; weigh 0.4g of astragalus polysaccharide Put it into a sterile beaker, add it to the spare mixed solution into the sterile beaker, stir to fully dissolve the astragalus polysaccharide, and obtain a sodium lactate mixed solution; before storing the embryos at room temperature, add penicillin, Streptomycin and calf serum can be used to obtain embryo room temperature preservation solution.
[0023] Use the embryo room temperature preservation solution of this embodiment...
Embodiment 2
[0025] A normal temperature preservation solution for equine animal embryos, including 5000ml of sodium lactate mixed solution as the main solution, and every 1000ml of sodium lactate Ringer injection contains 1 million units of penicillin, 1.2 million units of streptomycin and 3% inactivated horse serum by volume.
[0026] Weigh 10.5g of sodium lactate, 6g of sodium chloride, 13.5g of potassium chloride and 9g of calcium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 3 hours, and set aside; weigh 1.4g of astragalus polysaccharide into Add it to the sterile beaker, add it to the spare mixed solution, stir to fully dissolve the astragalus polysaccharide, and get the sodium lactate mixed solution; before storing the embryos at room temperature, add penicillin and streptomycin to the sodium lactate mixed solution according to the component content. And inactivated horse serum, the embryo room temperature preservation solution can be obtained.
...
Embodiment 3
[0029] A normal temperature preservation solution for equine animal embryos, including 4000ml of sodium lactate mixture as the main solution, wherein every 1000ml of sodium lactate mixture contains 800,000 units of penicillin, 800,000 units of streptomycin and 5% inactivated horse serum by volume; Wherein, the content of each component in the sodium lactate mixed solution is: every 1000ml sodium lactate mixed solution contains 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride.
[0030] Weigh 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride, add 4000ml of distilled water to mix, and place in a constant temperature box at 37°C for 1 hour to obtain a sodium lactate mixture for use; store the embryos at room temperature Before, add penicillin, streptomycin and inactivated horse serum to the sodium lactate mixture according to the component content to obtain embryo room tem...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com