Three-dimensional stress cell culture device capable of applying dynamic load

A three-dimensional force and dynamic load technology, applied in the field of cell culture, can solve the problems of cell communication in vivo and other problems, and achieve the effects of low manufacturing cost, easy operation, and small size

Active Publication Date: 2014-04-30
苏州智能制造研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the existing inventions and devices at present, the cells are mostly in the culture medium, which is quite different from the three-dimensional stress environment in which the cells in the human b

Method used

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  • Three-dimensional stress cell culture device capable of applying dynamic load
  • Three-dimensional stress cell culture device capable of applying dynamic load
  • Three-dimensional stress cell culture device capable of applying dynamic load

Examples

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specific example

[0066] The rod-shaped biological structure mixed with chondrocytes is stretched by the cell culture device of the present invention to realize the cultivation of chondrocytes.

[0067] Step 1: Mix the chondrocytes into the agarose solution, pour the uniformly mixed solution into a cylindrical mold, and form an active agarose gel bar containing chondrocytes by refrigerating at 4°C.

[0068] Step 2. Turn on the ultraviolet lamp. After a period of time, unlock the lock, pull out the clamping device inside the cell culture device, clamp the above-mentioned calcium alginate gel rod structure mixed with chondrocytes into the clamping device, and then put it into the clamping device. Put it inside the cylinder, lock the lock, connect the culture solution inlet port on the right side of the cell culture device, and connect the liquid outlet port on the left side.

[0069] Step 3. On the LCD screen, set the tensile force curve for the rod-shaped structure, the stretching frequency, the...

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Abstract

The invention discloses a three-dimensional stress cell culture device capable of applying a dynamic load. The device comprises a shell with a liquid outlet and a liquid inlet, a clamping device which is arranged in the shell and used for clamping a biological structure, a driving motor for providing a driving force to the clamping device, a heating device for heating a culture solution in the shell, a temperature sensor for detecting the temperature of the culture solution and outputting temperature signals, a tension sensor for detecting the tension of the biological structure and outputting tension signals, and a controller for receiving the temperature and tension signals and outputting control signals to the driving motor and the heating device according to the received signal information. The device disclosed by the invention is simple in structure, low in manufacturing cost and easy to operate. The device can carry out a force-controllable reciprocating stretching operation on a biological structure according to a set program to simulate an in vivo environment so as to culture cells through operations on a liquid crystal display screen after the biological structure mixed with cells is arranged in the cell culture device.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a three-dimensional force culture device for cells capable of applying dynamic loads. Background technique [0002] With the development of society, more and more people are engaged in the field of life science, which promotes the rapid development of life science. The organs, tissues, cells and biomacromolecules of the body undergo corresponding changes in shape and function under the action of mechanics, which is the body’s response process to mechanical stimuli, and the body’s self-adaptation to a certain range of mechanical stimuli plays a role in maintaining normal physiological functions. important role. Cells are composed of biological macromolecules and are the basic units of life activities. Studying the effect of force on cells is the basis for revealing the biomechanical properties of organs and tissues, and it is also the starting point for further research on ...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36
CPCC12M23/48C12M35/04C12M37/00C12M41/00C12M41/12C12M41/24C12M41/44
Inventor 贺永邵惠锋傅建中朱伟杰赖金涛
Owner 苏州智能制造研究院有限公司
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